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Coauthors
Cynthia Monaco Department of Medicine, Washington University School of Medicine, USA
1 protocol

Douglas S. Kwon
  • Ragon Institute of MGH, MIT, and Harvard, USA
Contributions
  • 1 Author merit

Education

Ph.D in Immunology and Microbiology, School of Medicine, New York University, USA, 2004

Current position

Assistant Professor, Harvard Medical School
Associate Member of Infectious Disease Program, Broad Institute
Assistant Physician, Division of Infectious Diseases, Massachusetts General Hospital
Associate Member, Mucosal Immunology Program, Centre for the Aids Programme of Research in South Africa (CAPRISA)
Faculty Member, Microbial Sciences Initiative, Harvard Medical School
Faculty Member, Immunology Department, Harvard Medical School
Curriculum design consultant, Kaplan, Inc.
Co-founder, Day Zero Diagnostics, Inc.

Publications (since 2014)

  1. Gosmann, C., Anahtar, M. N., Handley, S. A., Farcasanu, M., Abu-Ali, G., Bowman, B. A., Padavattan, N., Desai, C., Droit, L., Moodley, A., Dong, M., Chen, Y., Ismail, N., Ndung'u, T., Ghebremichael, M. S., Wesemann, D. R., Mitchell, C., Dong, K. L., Huttenhower, C., Walker, B. D., Virgin, H. W. and Kwon, D. S. (2017). Lactobacillus-Deficient Cervicovaginal Bacterial Communities Are Associated with Increased HIV Acquisition in Young South African Women. Immunity 46(1): 29-37.
  2. Gootenberg, D. B., Paer, J. M., Luevano, J. M. and Kwon, D. S. (2017). HIV-associated changes in the enteric microbial community: potential role in loss of homeostasis and development of systemic inflammation. Curr Opin Infect Dis 30(1): 31-43.
  3. Anahtar, M. N., Bowman, B. A. and Kwon, D. S. (2016). Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization. J Vis Exp(110).
  4. Monaco, C. L., Gootenberg, D. B., Zhao, G., Handley, S. A., Ghebremichael, M. S., Lim, E. S., Lankowski, A., Baldridge, M. T., Wilen, C. B., Flagg, M., Norman, J. M., Keller, B. C., Luevano, J. M., Wang, D., Boum, Y., Martin, J. N., Hunt, P. W., Bangsberg, D. R., Siedner, M. J., Kwon, D. S. and Virgin, H. W. (2016). Altered Virome and Bacterial Microbiome in Human Immunodeficiency Virus-Associated Acquired Immunodeficiency Syndrome. Cell Host Microbe 19(3): 311-322.
  5. Gandhi, R. T., Kwon, D. S., Macklin, E. A., Shopis, J. R., McLean, A. P., McBrine, N., Flynn, T., Peter, L., Sbrolla, A., Kaufmann, D. E., Porichis, F., Walker, B. D., Bhardwaj, N., Barouch, D. H. and Kavanagh, D. G. (2016). Immunization of HIV-1-Infected Persons With Autologous Dendritic Cells Transfected With mRNA Encoding HIV-1 Gag and Nef: Results of a Randomized, Placebo-Controlled Clinical Trial. J Acquir Immune Defic Syndr 71(3): 246-253.
  6. Riou, C., Strickland, N., Soares, A. P., Corleis, B., Kwon, D. S., Wherry, E. J., Wilkinson, R. J. and Burgers, W. A. (2016). HIV Skews the Lineage-Defining Transcriptional Profile of Mycobacterium tuberculosis-Specific CD4+ T Cells. J Immunol 196(7): 3006-3018.
  7. Siedner, M. J., Kim, J. H., Nakku, R. S., Hemphill, L., Triant, V. A., Haberer, J. E., Martin, J. N., Boum, Y., 2nd, Kwon, D. S., Tsai, A. C., Hunt, P. W., Okello, S. and Bangsberg, D. R. (2016). HIV infection and arterial stiffness among older-adults taking antiretroviral therapy in rural Uganda. AIDS 30(4): 667-670.
  8. Sips, M., Krykbaeva, M., Diefenbach, T. J., Ghebremichael, M., Bowman, B. A., Dugast, A. S., Boesch, A. W., Streeck, H., Kwon, D. S., Ackerman, M. E., Suscovich, T. J., Brouckaert, P., Schacker, T. W. and Alter, G. (2016). Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies. Mucosal Immunol 9(6): 1584-1595.
  9. Siedner, M. J., Kim, J. H., Nakku, R. S., Bibangambah, P., Hemphill, L., Triant, V. A., Haberer, J. E., Martin, J. N., Mocello, A. R., Boum, Y., 2nd, Kwon, D. S., Tracy, R. P., Burdo, T., Huang, Y., Cao, H., Okello, S., Bangsberg, D. R. and Hunt, P. W. (2016). Persistent Immune Activation and Carotid Atherosclerosis in HIV-Infected Ugandans Receiving Antiretroviral Therapy. J Infect Dis 213(3): 370-378.
  10. Byrne, E. H., Anahtar, M. N., Cohen, K. E., Moodley, A., Padavattan, N., Ismail, N., Bowman, B. A., Olson, G. S., Mabhula, A., Leslie, A., Ndung'u, T., Walker, B. D., Ghebremichael, M. S., Dong, K. L. and Kwon, D. S. (2016). Association between injectable progestin-only contraceptives and HIV acquisition and HIV target cell frequency in the female genital tract in South African women: a prospective cohort study. Lancet Infect Dis 16(4): 441-448.
  11. Gandhi, R. T., Kwon, D. S., Macklin, E. A., Shopis, J. R., McLean, A. P., McBrine, N., Flynn, T., Peter, L., Sbrolla, A., Kaufmann, D. E., Porichis, F., Walker, B. D., Bhardwaj, N., Barouch, D. H. and Kavanagh, D. G. (2016). Immunization of HIV-1-Infected Persons With Autologous Dendritic Cells Transfected With mRNA Encoding HIV-1 Gag and Nef: Results of a Randomized, Placebo-Controlled Clinical Trial. J Acquir Immune Defic Syndr 71(3): 246-253.
  12. Siedner, M. J., Kim, J. H., Nakku, R. S., Bibangambah, P., Hemphill, L., Triant, V. A., Haberer, J. E., Martin, J. N., Mocello, A. R., Boum, Y., 2nd, Kwon, D. S., Tracy, R. P., Burdo, T., Huang, Y., Cao, H., Okello, S., Bangsberg, D. R. and Hunt, P. W. (2016). Persistent Immune Activation and Carotid Atherosclerosis in HIV-Infected Ugandans Receiving Antiretroviral Therapy. J Infect Dis 213(3): 370-378.
  13. Gandhi, R. T., Kwon, D. S., Macklin, E. A., Shopis, J. R., McLean, A. P., McBrine, N., Flynn, T., Peter, L., Sbrolla, A., Kaufmann, D. E., Porichis, F., Walker, B. D., Bhardwaj, N., Barouch, D. H. and Kavanagh, D. G. (2016). Immunization of HIV-1-Infected Persons With Autologous Dendritic Cells Transfected With mRNA Encoding HIV-1 Gag and Nef: Results of a Randomized, Placebo-Controlled Clinical Trial. J Acquir Immune Defic Syndr 71(3): 246-253.
  14. Siedner, M. J., Kim, J. H., Nakku, R. S., Bibangambah, P., Hemphill, L., Triant, V. A., Haberer, J. E., Martin, J. N., Mocello, A. R., Boum, Y., 2nd, Kwon, D. S., Tracy, R. P., Burdo, T., Huang, Y., Cao, H., Okello, S., Bangsberg, D. R. and Hunt, P. W. (2016). Persistent Immune Activation and Carotid Atherosclerosis in HIV-Infected Ugandans Receiving Antiretroviral Therapy. J Infect Dis 213(3): 370-378.
  15. Adoro, S., Cubillos-Ruiz, J. R., Chen, X., Deruaz, M., Vrbanac, V. D., Song, M., Park, S., Murooka, T. T., Dudek, T. E., Luster, A. D., Tager, A. M., Streeck, H., Bowman, B., Walker, B. D., Kwon, D. S., Lazarevic, V. and Glimcher, L. H. (2015). IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun 6: 7562.
  16. Anahtar, M. N., Byrne, E. H., Doherty, K. E., Bowman, B. A., Yamamoto, H. S., Soumillon, M., Padavattan, N., Ismail, N., Moodley, A., Sabatini, M. E., Ghebremichael, M. S., Nusbaum, C., Huttenhower, C., Virgin, H. W., Ndung'u, T., Dong, K. L., Walker, B. D., Fichorova, R. N. and Kwon, D. S. (2015). Cervicovaginal bacteria are a major modulator of host inflammatory responses in the female genital tract. Immunity 42(5): 965-976.
  17. Sips, M., Krykbaeva, M., Bowman, B., Diefenbach, T., Kwon, D., Brouckaert, P. and Alter, G. (2014). Targeting Mucosal Fc-Fc Receptor Interactions as Vaccine Strategy against Mucosal HIV-transmission. AIDS Res Hum Retroviruses 30(S1): A63-A63.
  18. Porichis, F., Hart, M. G., Zupkosky, J., Barblu, L., Kwon, D. S., McMullen, A., Brennan, T., Ahmed, R., Freeman, G. J., Kavanagh, D. G. and Kaufmann, D. E. (2014). Differential impact of PD-1 and/or interleukin-10 blockade on HIV-1-specific CD4 T cell and antigen-presenting cell functions. J Virol 88(5): 2508-2518.
  19. Ogunniyi, A. O., Thomas, B. A., Politano, T. J., Varadarajan, N., Landais, E., Poignard, P., Walker, B. D., Kwon, D. S. and Love, J. C. (2014). Profiling human antibody responses by integrated single-cell analysis. Vaccine 32(24): 2866-2873.
  20. Ladinsky, M. S., Kieffer, C., Olson, G., Deruaz, M., Vrbanac, V., Tager, A. M., Kwon, D. S. and Bjorkman, P. J. (2014). Electron tomography of HIV-1 infection in gut-associated lymphoid tissue. PLoS Pathog 10(1): e1003899.
  21. Doherty, K. E., Anahtar, M., Ghebremichael, M., Thogabekale, C., Padavattan, N., Dong, K., Walker, B. D., Ndung'u, T. and Kwon, D. S. (2014). Effects of Intra-vaginal Drying Agents on Mucosal Immune Activation and Risk for HIV Acquisition in South African Women. AIDS Res Hum Retroviruses 30(S1): A230-A231.
  22. Corleis, B., Lisanti, A., Korner, C., Amero, M. A., Rosenberg, E. S., Allen, T. M., Altfeld, M. and Kwon, D. S. (2014) Protective Role of TLR3 Induced Human Beta Defensins during Acute HIV-1 Infection. AIDS Res Hum Retroviruses 30(S1): A124-A125.
  23. Byrne, E. H., Anahtar, M., Doherty, K., Olson, G., Bowman, B., Padavattan, N., Ndhlovu, Z., Ghebremichael, M., Walker, B., Ndung'u, T., Dong, K. and Kwon, D. S. (2014) Injectable Contraceptive Use Correlates with Increased HIV Target Cells at the Cervix in Young South African Women. AIDS Res Hum Retroviruses 30(S1): A54-A55.
  24. Angin, M., Klarenbeek, P. L., King, M., Sharma, S. M., Moodley, E. S., Rezai, A., Piechocka-Trocha, A., Toth, I., Chan, A. T., Goulder, P. J., Ndung'u, T., Kwon, D. S. and Addo, M. M. (2014). Regulatory T cells expanded from HIV-1-infected individuals maintain phenotype, TCR repertoire and suppressive capacity. PLoS One 9(2): e86920.
1 Protocol published
Next-generation Sequencing of the DNA Virome from Fecal Samples
Authors:  Cynthia L. Monaco and Douglas S. Kwon, date: 03/05/2017, view: 1400, Q&A: 0
Herein we describe a detailed protocol for DNA virome analysis of low input human stool samples (Monaco et al., 2016). This protocol is divided into four main steps: 1) stool samples are pulverized to evenly distribute microbial matter; 2) ...