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Coauthors
Hilary Nicholson Harvard Medical School, USA
1 protocol

Benjamin Housden Harvard Medical School, USA
1 protocol

Norbert Perrimon
  • Harvard Medical School, USA
  • 1 Author merit

Education

Ph.D in Developmental Genetics, University of Paris, France, 1983

Current position

Adjunct Professor & FlyBase PI, Molecular and Cellular Biology Department, Harvard University, USA

Publications (since 2016)

  1. Kim, K., Hung, R. J. and Perrimon, N. (2017). miR-263a Regulates ENaC to Maintain Osmotic and Intestinal Stem Cell Homeostasis in Drosophila. Dev Cell 40(1): 23-36.
  2. Hu, Y., Comjean, A., Roesel, C., Vinayagam, A., Flockhart, I., Zirin, J., Perkins, L., Perrimon, N. and Mohr, S. E. (2017). FlyRNAi.org-the database of the Drosophila RNAi screening center and transgenic RNAi project: 2017 update. Nucleic Acids Res 45(D1): D672-D678.
  3. Housden, B. E., Muhar, M., Gemberling, M., Gersbach, C. A., Stainier, D. Y., Seydoux, G., Mohr, S. E., Zuber, J. and Perrimon, N. (2017). Loss-of-function genetic tools for animal models: cross-species and cross-platform differences. Nat Rev Genet 18(1): 24-40.
  4. Droujinine, I. A. and Perrimon, N. (2016). Interorgan Communication Pathways in Physiology: Focus on Drosophila. Annu Rev Genet 50: 539-570.
  5. Vinayagam, A., Kulkarni, M. M., Sopko, R., Sun, X., Hu, Y., Nand, A., Villalta, C., Moghimi, A., Yang, X., Mohr, S. E., Hong, P., Asara, J. M. and Perrimon, N. (2016). An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling. Cell Rep 16(11): 3062-3074.
  6. Housden, B. E. and Perrimon, N. (2016). Cas9-Mediated Genome Engineering in Drosophila melanogaster. Cold Spring Harb Protoc 2016(9): pdb top086843.
  7. Housden, B. E. and Perrimon, N. (2016). Detection of Indel Mutations in Drosophila by High-Resolution Melt Analysis (HRMA). Cold Spring Harb Protoc 2016(9): pdb prot090795.
  8. Housden, B. E. and Perrimon, N. (2016). Design and Generation of Donor Constructs for Genome Engineering in Drosophila. Cold Spring Harb Protoc 2016(9): pdb prot090787.
  9. Housden, B. E., Hu, Y. and Perrimon, N. (2016). Design and Generation of Drosophila Single Guide RNA Expression Constructs. Cold Spring Harb Protoc 2016(9): pdb prot090779.
  10. Wang, H., Becuwe, M., Housden, B. E., Chitraju, C., Porras, A. J., Graham, M. M., Liu, X. N., Thiam, A. R., Savage, D. B., Agarwal, A. K., Garg, A., Olarte, M. J., Lin, Q., Frohlich, F., Hannibal-Bach, H. K., Upadhyayula, S., Perrimon, N., Kirchhausen, T., Ejsing, C. S., Walther, T. C. and Farese, R. V. (2016). Seipin is required for converting nascent to mature lipid droplets. Elife 5.
  11. Ammeux, N., Housden, B. E., Georgiadis, A., Hu, Y. and Perrimon, N. (2016). Mapping signaling pathway cross-talk in Drosophila cells. Proc Natl Acad Sci U S A 113(35): 9940-9945.
  12. Petsakou, A. and Perrimon, N. (2016). "ISN't Thirst Sweet?" Says the Fly. Cell 166(4): 796-797.
  13. Fagegaltier, D., Falciatori, I., Czech, B., Castel, S., Perrimon, N., Simcox, A. and Hannon, G. J. (2016). Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway. Genes Dev 30(14): 1623-1635.
  14. Parkhitko, A. A., Binari, R., Zhang, N., Asara, J. M., Demontis, F. and Perrimon, N. (2016). Tissue-specific down-regulation of S-adenosyl-homocysteine via suppression of dAhcyL1/dAhcyL2 extends health span and life span in Drosophila. Genes Dev 30(12): 1409-1422.
  15. Housden, B. E. and Perrimon, N. (2016). Comparing CRISPR and RNAi-based screening technologies. Nat Biotechnol 34(6): 621-623.
  16. Mohr, S. E., Hu, Y., Ewen-Campen, B., Housden, B. E., Viswanatha, R. and Perrimon, N. (2016). CRISPR guide RNA design for research applications. FEBS J 283(17): 3232-3238.
  17. Hunter, G. L., Hadjivasiliou, Z., Bonin, H., He, L., Perrimon, N., Charras, G. and Baum, B. (2016). Coordinated control of Notch/Delta signalling and cell cycle progression drives lateral inhibition-mediated tissue patterning. Development 143(13): 2305-2310.
  18. De Lella Ezcurra, A. L., Bertolin, A. P., Kim, K., Katz, M. J., Gandara, L., Misra, T., Luschnig, S., Perrimon, N., Melani, M. and Wappner, P. (2016). miR-190 Enhances HIF-Dependent Responses to Hypoxia in Drosophila by Inhibiting the Prolyl-4-hydroxylase Fatiga. PLoS Genet 12(5): e1006073.
  19. Harris, K. P., Zhang, Y. V., Piccioli, Z. D., Perrimon, N. and Littleton, J. T. (2016). The postsynaptic t-SNARE Syntaxin 4 controls traffic of Neuroligin 1 and Synaptotagmin 4 to regulate retrograde signaling. Elife 5.
  20. Chavez, A., Tuttle, M., Pruitt, B. W., Ewen-Campen, B., Chari, R., Ter-Ovanesyan, D., Haque, S. J., Cecchi, R. J., Kowal, E. J., Buchthal, J., Housden, B. E., Perrimon, N., Collins, J. J. and Church, G. (2016). Comparison of Cas9 activators in multiple species. Nat Methods 13(7): 563-567.
  21. Vinayagam, A., Gibson, T. E., Lee, H. J., Yilmazel, B., Roesel, C., Hu, Y., Kwon, Y., Sharma, A., Liu, Y. Y., Perrimon, N. and Barabasi, A. L. (2016). Controllability analysis of the directed human protein interaction network identifies disease genes and drug targets. Proc Natl Acad Sci U S A 113(18): 4976-4981.
  22. Doupe, D. P. and Perrimon, N. (2016). Toward a Systems Understanding of Signaling Pathway Function. Curr Top Dev Biol 117: 221-236.
  23. Perrimon, N., Bonini, N. M. and Dhillon, P. (2016). Fruit flies on the front line: the translational impact of Drosophila. Dis Model Mech 9(3): 229-231.
  24. Ma, M., Zhao, H., Zhao, H., Binari, R., Perrimon, N. and Li, Z. (2016). Wildtype adult stem cells, unlike tumor cells, are resistant to cellular damages in Drosophila. Dev Biol 411(2): 207-216.
  25. Breitkopf, S. B., Yang, X., Begley, M. J., Kulkarni, M., Chiu, Y. H., Turke, A. B., Lauriol, J., Yuan, M., Qi, J., Engelman, J. A., Hong, P., Kontaridis, M. I., Cantley, L. C., Perrimon, N. and Asara, J. M. (2016). A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2. Sci Rep 6: 20471.
1 Protocol published
Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells
Authors:  Benjamin E. Housden, Hilary E. Nicholson and Norbert Perrimon, date: 02/05/2017, view: 2205, Q&A: 0
A synthetic lethal interaction is a type of genetic interaction where the disruption of either of two genes individually has little effect but their combined disruption is lethal. Knowledge of synthetic lethal interactions can allow for elucidation ...