Norbert Perrimon Harvard Medical School, USA
1 protocol

Hilary Nicholson Harvard Medical School, USA
1 protocol

Benjamin E. Housden
  • Harvard Medical School, USA
  • 1 Author merit


Ph.D in Developmental Biology, University of Cambridge, UK, 2010

Current position

Postdoctoral Fellow, Perrimon lab, Harvard Medical School, Boston, USA
Research Fellow, University of Exeter, UK

Publications (since 2014)

  1. Housden, B. E., Muhar, M., Gemberling, M., Gersbach, C. A., Stainier, D. Y., Seydoux, G., Mohr, S. E., Zuber, J. and Perrimon, N. (2017). Loss-of-function genetic tools for animal models: cross-species and cross-platform differences. Nat Rev Genet 18(1): 24-40.
  2. Housden, B. E. and Perrimon, N. (2016). Cas9-Mediated Genome Engineering in Drosophila melanogaster. Cold Spring Harb Protoc 2016(9): pdb top086843.
  3. Housden, B. E. and Perrimon, N. (2016). Detection of Indel Mutations in Drosophila by High-Resolution Melt Analysis (HRMA). Cold Spring Harb Protoc 2016(9): pdb prot090795.
  4. Housden, B. E. and Perrimon, N. (2016). Design and Generation of Donor Constructs for Genome Engineering in Drosophila. Cold Spring Harb Protoc 2016(9): pdb prot090787.
  5. Housden, B. E., Hu, Y. and Perrimon, N. (2016). Design and Generation of Drosophila Single Guide RNA Expression Constructs. Cold Spring Harb Protoc 2016(9): pdb prot090779.
  6. Wang, H., Becuwe, M., Housden, B. E., Chitraju, C., Porras, A. J., Graham, M. M., Liu, X. N., Thiam, A. R., Savage, D. B., Agarwal, A. K., Garg, A., Olarte, M. J., Lin, Q., Frohlich, F., Hannibal-Bach, H. K., Upadhyayula, S., Perrimon, N., Kirchhausen, T., Ejsing, C. S., Walther, T. C. and Farese, R. V. (2016). Seipin is required for converting nascent to mature lipid droplets. Elife 5.
  7. Ammeux, N., Housden, B. E., Georgiadis, A., Hu, Y. and Perrimon, N. (2016). Mapping signaling pathway cross-talk in Drosophila cells. Proc Natl Acad Sci U S A 113(35): 9940-9945.
  8. Housden, B. E. and Perrimon, N. (2016). Comparing CRISPR and RNAi-based screening technologies. Nat Biotechnol 34(6): 621-623.
  9. Mohr, S. E., Hu, Y., Ewen-Campen, B., Housden, B. E., Viswanatha, R. and Perrimon, N. (2016). CRISPR guide RNA design for research applications. FEBS J 283(17): 3232-3238.
  10. Chavez, A., Tuttle, M., Pruitt, B. W., Ewen-Campen, B., Chari, R., Ter-Ovanesyan, D., Haque, S. J., Cecchi, R. J., Kowal, E. J., Buchthal, J., Housden, B. E., Perrimon, N., Collins, J. J. and Church, G. (2016). Comparison of Cas9 activators in multiple species. Nat Methods 13(7): 563-567.
  11. Zacharioudaki, E., Housden, B. E., Garinis, G., Stojnic, R., Delidakis, C. and Bray, S. J. (2016). Genes implicated in stem cell identity and temporal programme are directly targeted by Notch in neuroblast tumours. Development 143(2): 219-231.
  12. Housden, B. E., Valvezan, A. J., Kelley, C., Sopko, R., Hu, Y., Roesel, C., Lin, S., Buckner, M., Tao, R., Yilmazel, B., Mohr, S. E., Manning, B. D. and Perrimon, N. (2015). Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi. Sci Signal 8(393): rs9.
  13. Lin, S., Ewen-Campen, B., Ni, X., Housden, B. E. and Perrimon, N. (2015). In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila. Genetics 201(2): 433-442.
  14. Chavez, A., Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., E, P. R. I., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn, N., Housden, B. E., Perrimon, N., Weiss, R., Aach, J., Collins, J. J. and Church, G. M. (2015). Highly efficient Cas9-mediated transcriptional programming. Nat Methods 12(4): 326-328.
  15. Housden, B. E., Lin, S. and Perrimon, N. (2014). Cas9-based genome editing in Drosophila. Methods Enzymol 546: 415-439.
  16. Housden, B. E. and Perrimon, N. (2014). Spatial and temporal organization of signaling pathways. Trends Biochem Sci 39(10): 457-464.
  17. Li, J., Housden, B. E. and Bray, S. J. (2014). Notch signaling assays in Drosophila cultured cell lines. Methods Mol Biol 1187: 131-141.
  18. Housden, B. E., Li, J. and Bray, S. J. (2014). Visualizing Notch signaling in vivo in Drosophila tissues. Methods Mol Biol 1187: 101-113.
  19. Simon, R., Aparicio, R., Housden, B. E., Bray, S. and Busturia, A. (2014). Drosophila p53 controls Notch expression and balances apoptosis and proliferation. Apoptosis 19(10): 1430-1443.
  20. Mohr, S. E., Hu, Y., Kim, K., Housden, B. E. and Perrimon, N. (2014). Resources for functional genomics studies in Drosophila melanogaster. Genetics 197(1): 1-18.
  21. Housden, B. E., Terriente-Felix, A. and Bray, S. J. (2014). Context-dependent enhancer selection confers alternate modes of notch regulation on argos. Mol Cell Biol 34(4): 664-672.
1 Protocol published
Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells
Authors:  Benjamin E. Housden, Hilary E. Nicholson and Norbert Perrimon, date: 02/05/2017, view: 2040, Q&A: 0
A synthetic lethal interaction is a type of genetic interaction where the disruption of either of two genes individually has little effect but their combined disruption is lethal. Knowledge of synthetic lethal interactions can allow for elucidation ...