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Coauthors
Kirankumar Mysore Plant Biology Division, the Samuel Roberts Noble Foundation, USA
3 protocols

Yuki Ichinose Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life Science, Okayama University, Japan
1 protocol

Takako Ishiga Faculty of Life and Environmental Sciences, University of Tsukuba, Japan
1 protocol

Yasuhiro Ishiga
  • Faculty of Life and Environmental Sciences, University of Tsukuba, Japan
  • 1 Author merit

Education

Ph.D. in Agriculture, Okayama University, Okayama, Japan, 2005

Current position

Assistant Professor, University of Tsukuba, Tsukuba, Japan

Publications

  1. Ishiga, Y., Ishiga, T., Ikeda, Y., Matsuura, T. and Mysore, K. S. (2016). NADPH-dependent thioredoxin reductase C plays a role in nonhost disease resistance against Pseudomonas syringae pathogens by regulating chloroplast-generated reactive oxygen species. PeerJ 4: e1938.
  2. Toyoda, K., Yao, S., Takagi, M., Uchioki, M., Miki, M., Tanaka, K., Suzuki, T., Amano, M., Kiba, A., Kato, T., Takahashi, H., Ishiga, Y., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y. and Shiraishi, T. (2016). Plant cell wall as a site for molecular contacts in fungal pathogenesis. Physiological and Molecular Plant Pathology 95: 44-49.
  3. Takagi, H., Ishiga, Y., Watanabe, S., Konishi, T., Egusa, M., Akiyoshi, N., Matsuura, T., Mori, I., Hirayama, T., Kaminaka, H., Shimada, H. and Sakamoto, A. (2016). Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner. Journal of Experimental Botany 678: 2519-32.
  4. Ishiga, Y. and Ichinose, Y. (2016). Pseudomonas syringae pv. tomato OxyR Is Required for Virulence in Tomato and Arabidopsis. Mol Plant Microbe Interact 29(2): 119-131.
  5. Ishiga, Y., Uppalapati, S. R., Gill, U. S., Huhman, D., Tang, Y. and Mysore, K. S. (2015). Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust. Scientific Reports 12(5): 13061.
  6. Lee, S., Rojas, C. M., Ishiga, Y., Pandey, S. and Mysore, K. S. (2013). Arabidopsis heterotrimeric G-proteins play a critical role in host and nonhost resistance against Pseudomonas syringae pathogens. PLoS One 8(12): e82445.
  7. Ishiga, Y., Upplapapti, S. and Mysore, K. S. (2013). Expression analysis reveals a role for hydrophobic or epicuticular wax signals in pre-penetration structure formation of Phakopsora pachyrhizi. Plant Signal Behav 8(11): e26959.
  8. Ishiga, Y., Ishiga, T., Uppalapati, S. R. and Mysore, K. S. (2013). Jasmonate ZIM-domain (JAZ) protein regulates host and nonhost pathogen-induced cell death in tomato and Nicotiana benthamiana. PLoS One 8(9): e75728.
  9. Ramegowda, V., Senthil-Kumar, M., Ishiga, Y., Kaundal, A., Udayakumar, M. and Mysore, K. S. (2013). Drought stress acclimation imparts tolerance to Sclerotinia sclerotiorum and Pseudomonas syringae in Nicotiana benthamiana. Int J Mol Sci 14(5): 9497-9513.
  10. Lee, S., Ishiga, Y., Clermont, K. and Mysore, K. S. (2013). Coronatine inhibits stomatal closure and delays hypersensitive response cell death induced by nonhost bacterial pathogens. PeerJ 1: e34.
  11. Uppalapati, S. R., Serba, D. D., Ishiga, Y., Szabo, L. J., Mittal, S., Bhandari, H. S., Bouton, J. H., Mysore, K. S. and Saha, M. C. (2013). Characterization of the rust fungus, Puccinia emaculata, and evaluation of genetic variability for rust resistance in switchgrass populations. BioEnergy Research 6: 458-468.
  12. Uppalapati, S. R., Ishiga, Y., Doraiswamy, V., Bedair, M., Mittal, S., Chen, J., Nakashima, J., Tang, Y., Tadege, M., Ratet, P., Chen, R., Schultheiss, H. and Mysore, K. S. (2012). Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens. Plant Cell 24(1): 353-370.
  13. Ishiga, Y., Ishiga, T., Wangdi, T., Mysore, K. S. and Uppalapati, S. R. (2012). NTRC and chloroplast-generated reactive oxygen species regulate Pseudomonas syringae pv. tomato disease development in tomato and Arabidopsis. Mol Plant Microbe Interact 25(3): 294-306.
  14. Ishiga, Y., Ishiga, T., Uppalapati, S. R. and Mysore, K. S. (2011). Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions. Plant Methods 7: 32.
  15. Ishiga, Y., Uppalapati, S. R., Ishiga, T. and Mysore, K. S. (2011). Involvement of SGT1 in COR-mediated signal transduction pathway leading to disease symptom development. Plant Signal Behav 6(7): 1072-1073.
  16. Uppalapati, S. R., Ishiga, Y., Ryu, C. M., Ishiga, T., Wang, K., Noel, L. D., Parker, J. E. and Mysore, K. S. (2011). SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis. New Phytol 189(1): 83-93.
  17. Ishiga, Y., Uppalapati, S. R., Ishiga, T. and Bender, C. L. (2010). Exogenous coronatine, but not coronafacic acid or methyl jasmonate, restores the disease phenotype of a coronatine defective mutant of Pseudomonas syringae pv. tomato on tomato seedlings. J Gen Plant Pathol 76: 188-195.
  18. Yakushiji, S., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2009). Bacterial DNA activates immunity in Arabidopsis thaliana. J Gen Plant Pathol 75: 227-234.
  19. Ishiga, Y., Uppalapati, S. R., Ishiga, T., Elavarthi, S., Martin, B. and Bender, C. L. (2009). Involvement of coronatine-inducible reactive oxygen species in bacterial speck disease of tomato. Plant Signal Behav 4(3): 237-239.
  20. Ishiga, Y., Uppalapati, S. R., Ishiga, T., Elavarthi, S., Martin, B. and Bender, C. L. (2009). The phytotoxin coronatine induces light-dependent reactive oxygen species in tomato seedlings. New Phytol 181(1): 147-160.
  21. Uppalapati, S. R., Ishiga, Y., Wangdi, T., Urbanczyk-Wochniak, E., Ishiga, T., Mysore, K. S. and Bender, C. L. (2008). Pathogenicity of Pseudomonas syringae pv. tomato on tomato seedlings: phenotypic and gene expression analyses of the virulence function of coronatine. Mol Plant Microbe Interact 21(4): 383-395.
  22. Higashi, K., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2008). Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana. Mol Genet Genomics 279(3): 303-312.
  23. Uppalapati, S. R., Ishiga, Y., Wangdi, T., Kunkel, B. N., Anand, A., Mysore, K. S. and Bender, C. L. (2007). The phytotoxin coronatine contributes to pathogen fitness and is required for suppression of salicylic acid accumulation in tomato inoculated with Pseudomonas syringae pv. tomato DC3000. Mol Plant Microbe Interact 20(8): 955-965.
  24. Naito, K., Ishiga, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2007). N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana. J Gen Plant Pathol 73: 281-285.
  25. Ishiga, Y., Takeuchi, K., Taguchi, F., Inagaki, Y., Toyoda, K. and Ichinose, Y. (2005). Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (∆fliC) and flagellin-glycosylation (∆orf1). J Gen Plant Pathol 71: 302-307.
  26. Uppalapati, S. R., Toyoda, K., Ishiga, Y., Ichinose, Y. and Shiraishi, T. (2004). Differential regulation of MBP kinases by a glycoprotein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea. Physiol Mol Plant Pathol 64: 17-25.
  27. Matsui, H., Nakamura, G., Ishiga, Y., Toshima, H., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2004). Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products. Mol Genet Genomics 271(1): 1-10.
  28. Ichinose, Y., Taguchi, F., Takeuchi, K., Marutani, M., Ishiga, Y., Inagaki, Y., Toyoda, K. and Shiraishi, T. (2004). Bacterial flagellins as elicitors of the defense response. In: Tsuyumu, S., Leach, J. E., Shiraishi, T. and Wolpert, T. (eds). Genomic and Genetic Analysis of Plant Parasitism and Defense p.83-91.
  29. Ishiga, Y., Matsui, H., Nakamura, G., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2004). Compatible-specific expression of 12-oxophytodienoic acid 10,11-reductase gene in pea. In: Tsuyumu, S., Leach, J. E., Shiraishi, T. and Wolpert, T. (eds). Genomic and Genetic Analysis of Plant Parasitism and Defense p. 287.
  30. Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2003). Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens. J Gen Plant Pathol 69: 351-357.
  31. Ishiga, Y., Funato, A., Tachiki, T., Toyoda, K., Shiraishi, T., Yamada, T. and Ichinose, Y. (2002). Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen. Plant Cell Physiol 43(10): 1210-1220.
  32. Ichinose, Y., Hisayasu, Y., Sanematsu, S., Ishiga, Y., Seki, H., Toyoda, K., Shiraishi, T. and Yamada, T. (2001). Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J. Plant Sci 160(5): 997-1006.
1 Protocol published
Pseudomonas syringae Flood-inoculation Method in Arabidopsis
Authors:  Yasuhiro Ishiga, Takako Ishiga, Yuki Ichinose and Kirankumar S. Mysore, date: 01/20/2017, view: 1857, Q&A: 0
Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we ...