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Coauthors
Raghuvir Tomar Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, India
1 protocol

Sakshi Chauhan Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, India
1 protocol

Gajendra Kumar Azad
  • Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, India
Contributions
  • 1 Author merit

Education

Ph.D in Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal(IISERB), India, 2014

Current Position

Postdoctoral Research fellow in Department of Genetics, The Hebrew University of Jerusalem, Israel

Publications

  1. Sariki, S. K., Sahu, P. K., Golla, U., Singh, V., Azad, G. K. and Tomar, R. S. (2016). Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae. FEBS J 283(22): 4056-4083.
  2. Azad, G. K. and Tomar, R. S. (2016). The multifunctional transcription factor Rap1: a regulator of yeast physiology. Front Biosci (Landmark Ed) 21: 918-930.
  3. Azad, G. K. and Tomar, R. S. (2016). Partial purification of histone H3 proteolytic activity from the budding yeast Saccharomyces cerevisiae. Yeast 33(6): 217-226.
  4. Singh, V., Azad, G. K., Sariki, S. K. and Tomar, R. S. (2015). Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p. FEBS Lett 589(20 Pt B): 3165-3174.
  5. Azad, G. K., Singh, V., Baranwal, S., Thakare, M. J. and Tomar, R. S. (2015). The transcription factor Rap1p is required for tolerance to cell-wall perturbing agents and for cell-wall maintenance in Saccharomyces cerevisiae. FEBS Lett 589(1): 59-67.
  6. Azad, G. K. and Tomar, R. S. (2015). Epigenetics of Curcumin: A gifted dietary therapeutics compound. J Carcinog Mutagen 6: 206.
  7. Azad, G. K. and Tomar, R. S. (2014). Proteolytic clipping of histone tails: the emerging role of histone proteases in regulation of various biological processes. Mol Biol Rep 41(5): 2717-2730.
  8. Azad, G. K. and Tomar, R. S. (2014). Ebselen, a promising antioxidant drug: mechanisms of action and targets of biological pathways. Mol Biol Rep 41(8): 4865-4879.
  9. Azad, G. K., Singh, V., Thakare, M. J., Baranwal, S. and Tomar, R. S. (2014). Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae. BMC Microbiol 14: 317-327.
  10. Baranwal, S., Azad, G. K., Singh, V. and Tomar, R. S. (2014). Signaling of chloroquine-induced stress in the yeast Saccharomyces cerevisiae requires the Hog1 and Slt2 mitogen-activated protein kinase pathways. Antimicrob Agents Chemother 58(9): 5552-5566.
  11. Azad, G. K., Singh, V. and Tomar, R. S. (2014). Assessment of the biological pathways targeted by isocyanate using N-succinimidyl N-methylcarbamate in budding yeast Saccharomyces cerevisiae. PLoS One 9(3): e92993.
  12. Azad, G. K., Singh, V., Mandal, P., Singh, P., Golla, U., Baranwal, S., Chauhan, S. and Tomar, R. S. (2014). Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target. FEBS Open Bio 4: 77-89.
  13. Singh, V., Azad, G. K., Mandal, P., Reddy, M. A. and Tomar, R. S. (2014). Anti-cancer drug KP1019 modulates epigenetics and induces DNA damage response in Saccharomyces cerevisiae. FEBS Lett 588(6): 1044-1052.
  14. Singh, V., Azad, G. K., Reddy, M. A., Baranwal, S. and Tomar, R. S. (2014). Anti-cancer drug KP1019 induces Hog1 phosphorylation and protein ubiquitylation in Saccharomyces cerevisiae. Eur J Pharmacol 736: 77-85.
  15. Balkrishna, S. J., Kumar, S., Azad, G. K., Bhakuni, B. S., Panini, P., Ahalawat, N., Tomar, R. S., Detty, M. R. and Kumar, S. (2014). An ebselen like catalyst with enhanced GPx activity via a selenol intermediate. Org Biomol Chem 12(8): 1215-1219.
  16. Mandal, P., Verma, N., Azad, G. K., Singh, V., Golla, U. and Tomar, R. S. (2014). Epigenetics: Role of histone proteases in cellular functions and diseases. In: Maulik, N. and Karagiannis, T. (Eds.). Molecular Mechanisms and Physiology of Disease: Implications for Epigenetics and Health, Springer.
  17. Golla, U., Singh, V., Azad, G. K., Singh, P., Verma, N., Mandal, P., Chauhan, S. and Tomar, R. S. (2013). Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae. PLoS One 8(5): e64798.
  18. Azad, G. K., Singh, V., Golla, U. and Tomar, R. S. (2013). Depletion of cellular iron by curcumin leads to alteration in histone acetylation and degradation of Sml1p in Saccharomyces cerevisiae. PLoS One 8(3): e59003.
  19. Mandal, P., Azad, G. K. and Tomar, R. S. (2012). Identification of a novel histone H3 specific protease activity in nuclei of chicken liver. Biochem Biophys Res Commun 421(2): 261-267.
  20. Azad, G. K., Balkrishna, S. J., Sathish, N., Kumar, S. and Tomar, R. S. (2012). Multifunctional Ebselen drug functions through the activation of DNA damage response and alterations in nuclear proteins. Biochem Pharmacol 83(2): 296-303.
1 Protocol published
In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease
Authors:  Sakshi Chauhan, Gajendra Kumar Azad and Raghuvir Singh Tomar, date: 01/05/2017, view: 2078, Q&A: 0
Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and ...