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Coauthors
Ilona Kammerl Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
1 protocol

Hermen Overkleeft Department of Bio-organic Synthesis, Leiden University, The Netherlands
1 protocol

Bogdan Florea Department of Bio-organic Synthesis, Leiden University, The Netherlands
1 protocol

Oliver Vosyka Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
1 protocol

Silke Meiners
  • Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
  • 1 Author merit

Education

Ph.D in Max-Delbrück-Center for Molecular Medicine (MDC), Berlin, Germany

Current Position

Research Group Leader, Comprehensive Pneumology Center, Helmholtz Zentrum München German Research Center of Environmental Health, München, Germany

Publications (since 2010)

  1. Meiners, S. and Hilgendorff, A. (2016). Early injury of the neonatal lung contributes to premature lung aging: a hypothesis. Mol Cell Pediatr 3(1): 24.
  2. Kammerl, I. E. and Meiners, S. (2016). Proteasome function shapes innate and adaptive immune responses. Am J Physiol Lung Cell Mol Physiol 311(2): L328-336.
  3. Mossina, A., Lukas, C., Merl-Pham, J., Uhl, F. E., Mutze, K., Schamberger, A., Staab-Weijnitz, C., Jia, J., Yildirim, A. Ö., Königshoff, M., Hauck, S. M., Eickelberg, O. and Meiners, S. (2016). Cigarette smoke alters the secretome of lung epithelial cells. Proteomics.
  4. Wuttke, S., Zimpel, A., Bein, T., Braig, S., Stoiber, K., Vollmar, A., Muller, D., Haastert-Talini, K., Schaeske, J., Stiesch, M., Zahn, G., Mohmeyer, A., Behrens, P., Eickelberg, O., Bolukbas, D. A. and Meiners, S. (2016). Validating Metal-Organic Framework Nanoparticles for Their Nanosafety in Diverse Biomedical Applications. Adv Healthc Mater.
  5. Welk, V., Coux, O., Kleene, V., Abeza, C., Trümbach, D., Eickelberg, O. and Meiners, S. (2016). Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem 291(25): 13147-13159.
  6. van Rijt, S. H., Bölükbas, D. A., Argyo, C., Wipplinger, K., Naureen, M., Datz, S., Eickelberg, O., Meiners, S., Bein, T., Schmid, O. and Stoeger, T. (2016). Applicability of avidin protein coated mesoporous silica nanoparticles as drug carriers in the lung. Nanoscale 8(15): 8058-8069.
  7. Chen, S., Kammerl, I. E., Vosyka, O., Baumann, T., Yu, Y., Wu, Y., Irmler, M., Overkleeft, H. S., Beckers, J., Eickelberg, O., Meiners, S. and Stoeger, T. (2016). Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages. Cell Death Differ 23(6): 1026-1037.
  8. Kammerl, I. E., Dann, A., Mossina, A., Brech, D., Lukas, C., Vosyka, O., Nathan, P., Conlon, T. M., Wagner, D. E., Overkleeft, H. S., Prasse, A., Rosas, I. O., Straub, T., Krauss-Etschmann, S., Konigshoff, M., Preissler, G., Winter, H., Lindner, M., Hatz, R., Behr, J., Heinzelmann, K., Yildirim, A. O., Noessner, E., Eickelberg, O. and Meiners, S. (2016). Impairment of Immunoproteasome Function by Cigarette Smoke and in Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med 193(11): 1230-1241.
  9. Conlon, T. M., Bartel, J., Ballweg, K., Gunter, S., Prehn, C., Krumsiek, J., Meiners, S., Theis, F. J., Adamski, J., Eickelberg, O. and Yildirim, A. O. (2016). Metabolomics screening identifies reduced L-carnitine to be associated with progressive emphysema. Clin Sci (Lond) 130(4): 273-287.
  10. Caniard, A., Ballweg, K., Lukas, C., Yildirim, A. O., Eickelberg, O. and Meiners, S. (2015). Proteasome function is not impaired in healthy aging of the lung. Aging (Albany NY) 7(10): 776-792.
  11. Semren, N., Habel-Ungewitter, N. C., Fernandez, I. E., Konigshoff, M., Eickelberg, O., Stoger, T. and Meiners, S. (2015). Validation of the 2nd Generation Proteasome Inhibitor Oprozomib for Local Therapy of Pulmonary Fibrosis. PLoS One 10(9): e0136188.
  12. Oehrle, B., Burgstaller, G., Irmler, M., Dehmel, S., Grun, J., Hwang, T., Krauss-Etschmann, S., Beckers, J., Meiners, S. and Eickelberg, O. (2015). Validated prediction of pro-invasive growth factors using a transcriptome-wide invasion signature derived from a complex 3D invasion assay. Sci Rep 5: 12673.
  13. Semren, N., Welk, V., Korfei, M., Keller, I. E., Fernandez, I. E., Adler, H., Gunther, A., Eickelberg, O. and Meiners, S. (2015). Regulation of 26S Proteasome Activity in Pulmonary Fibrosis. Am J Respir Crit Care Med 192(9): 1089-1101.
  14. Keller, I. E., Vosyka, O., Takenaka, S., Kloss, A., Dahlmann, B., Willems, L. I., Verdoes, M., Overkleeft, H. S., Marcos, E., Adnot, S., Hauck, S. M., Ruppert, C., Gunther, A., Herold, S., Ohno, S., Adler, H., Eickelberg, O. and Meiners, S. (2015). Regulation of immunoproteasome function in the lung. Sci Rep 5: 10230.
  15. Uhl, F. E., Vierkotten, S., Wagner, D. E., Burgstaller, G., Costa, R., Koch, I., Lindner, M., Meiners, S., Eickelberg, O. and Konigshoff, M. (2015). Preclinical validation and imaging of Wnt-induced repair in human 3D lung tissue cultures. Eur Respir J 46(4): 1150-1166.
  16. van Rijt, S. H., Bolukbas, D. A., Argyo, C., Datz, S., Lindner, M., Eickelberg, O., Konigshoff, M., Bein, T. and Meiners, S. (2015). Protease-mediated release of chemotherapeutics from mesoporous silica nanoparticles to ex vivo human and mouse lung tumors. ACS Nano 9(3): 2377-2389.
  17. Bolukbas, D. A. and Meiners, S. (2015). Lung cancer nanomedicine: potentials and pitfalls. Nanomedicine (Lond) 10(21): 3203-3212.
  18. Meiners, S., Eickelberg, O. and Konigshoff, M. (2015). Hallmarks of the ageing lung. Eur Respir J 45(3): 807-827.
  19. Thannickal, V. J., Murthy, M., Balch, W. E., Chandel, N. S., Meiners, S., Eickelberg, O., Selman, M., Pardo, A., White, E. S., Levy, B. D., Busse, P. J., Tuder, R. M., Antony, V. B., Sznajder, J. I. and Budinger, G. R. (2015). Blue journal conference. Aging and susceptibility to lung disease. Am J Respir Crit Care Med 191(3): 261-269.
  20. Meiners, S. and Ballweg, K. (2014). Proteostasis in pediatric pulmonary pathology. Mol Cell Pediatr 1(1): 11.
  21. Ballweg, K., Mutze, K., Konigshoff, M., Eickelberg, O. and Meiners, S. (2014). Cigarette smoke extract affects mitochondrial function in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 307(11): L895-907.
  22. Meiners, S. and Greene, C. M. (2014). Protein quality control in lung disease: it's all about cloud networking. Eur Respir J 44(4): 846-849.
  23. Schamberger, A. C., Mise, N., Meiners, S. and Eickelberg, O. (2014). Epigenetic mechanisms in COPD: implications for pathogenesis and drug discovery. Expert Opin Drug Discov 9(6): 609-628.
  24. van Rijt, S. H., Bein, T. and Meiners, S. (2014). Medical nanoparticles for next generation drug delivery to the lungs. Eur Respir J 44(3): 765-774.
  25. Meiners, S., Keller, I. E., Semren, N. and Caniard, A. (2014). Regulation of the proteasome: evaluating the lung proteasome as a new therapeutic target. Antioxid Redox Signal 21(17): 2364-2382.
  26. Lenz, A. G., Stoeger, T., Cei, D., Schmidmeir, M., Semren, N., Burgstaller, G., Lentner, B., Eickelberg, O., Meiners, S. and Schmid, O. (2014). Efficient bioactive delivery of aerosolized drugs to human pulmonary epithelial cells cultured in air-liquid interface conditions. Am J Respir Cell Mol Biol 51(4): 526-535.
  27. Schamberger, A. C., Mise, N., Jia, J., Genoyer, E., Yildirim, A. O., Meiners, S. and Eickelberg, O. (2014). Cigarette smoke-induced disruption of bronchial epithelial tight junctions is prevented by transforming growth factor-beta. Am J Respir Cell Mol Biol 50(6): 1040-1052.
  28. Meiners, S. (2014). Protein Quality Control Coming of Age. In: Rojas, M., Meiners, S. and Le Saux, C. J. (eds). Molecular Aspects of Aging: Understanding Lung Aging, John Wiley & Sons.
  29. Korfei, M., von der Beck, D., Henneke, I., Markart, P., Ruppert, C., Mahavadi, P., Ghanim, B., Klepetko, W., Fink, L., Meiners, S., Kramer, O. H., Seeger, W., Vancheri, C. and Guenther, A. (2013). Comparative proteome analysis of lung tissue from patients with idiopathic pulmonary fibrosis (IPF), non-specific interstitial pneumonia (NSIP) and organ donors. J Proteomics 85: 109-128.
  30. van Rijt, S. H., Keller, I. E., John, G., Kohse, K., Yildirim, A. O., Eickelberg, O. and Meiners, S. (2012). Acute cigarette smoke exposure impairs proteasome function in the lung. Am J Physiol Lung Cell Mol Physiol 303(9): L814-823.
  31. Meiners, S. and Eickelberg, O. (2012). Next-generation personalized drug discovery: the tripeptide GHK hits center stage in chronic obstructive pulmonary disease. Genome Med 4(9): 70.
  32. Meiners, S. and Eickelberg, O. (2012). What shall we do with the damaged proteins in lung disease? Ask the proteasome! Eur Respir J 40(5): 1260-1268.
  33. Bieler, S., Hammer, E., Gesell-Salazar, M., Volker, U., Stangl, K. and Meiners, S. (2012). Low dose proteasome inhibition affects alternative splicing. J Proteome Res 11(8): 3947-3954.
  34. Dreger, H., Westphal, K., Wilck, N., Baumann, G., Stangl, V., Stangl, K. and Meiners, S. (2010). Protection of vascular cells from oxidative stress by proteasome inhibition depends on Nrf2. Cardiovasc Res 85(2): 395-403.
  35. Kloss, A., Meiners, S., Ludwig, A. and Dahlmann, B. (2010). Multiple cardiac proteasome subtypes differ in their susceptibility to proteasome inhibitors. Cardiovasc Res 85(2): 367-375.
1 Protocol published
Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits
Activity-based probes (ABP) are small organic molecules that irreversibly bind to the active center of a specific enzyme family and may be coupled to a fluorophore or an affinity tag (Li et al., 2013). Here, we describe a method to ...