He Huang Donald Danforth Plant Science Center, USA
1 protocol

Dmitri Anton Nusinow
  • Donald Danforth Plant Science Center, USA
  • 1 Author merit


Ph.D in Biochemistry and Molecular Biology, University of California, San Francisco, 2006

Current Position

Assistant Member and Principal Investigator, Donald Danforth Plant Science Center


  1. Huang, H. and Nusinow, D. A. (2016). Into the Evening: Complex Interactions in the Arabidopsis Circadian Clock. Trends Genet 32(10): 674-686.
  2. Mutka, A. M., Fentress, S. J., Sher, J. W., Berry, J. C., Pretz, C., Nusinow, D. A. and Bart, R. (2016). Quantitative, Image-Based Phenotyping Methods Provide Insight into Spatial and Temporal Dimensions of Plant Disease. Plant Physiol 172(2): 650-660.
  3. Huang, H., Alvarez, S. and Nusinow, D. A. (2016). Data on the identification of protein interactors with the Evening Complex and PCH1 in Arabidopsis using tandem affinity purification and mass spectrometry (TAP-MS). Data Brief 8: 56-60.
  4. Huang, H., Yoo, C. Y., Bindbeutel, R., Goldsworthy, J., Tielking, A., Alvarez, S., Naldrett, M. J., Evans, B. S., Chen, M. and Nusinow, D. A. (2016). PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. Elife 5: e13292.
  5. Kaiserli, E., Paldi, K., O'Donnell, L., Batalov, O., Pedmale, U. V., Nusinow, D. A., Kay, S. A. and Chory, J. (2015). Integration of Light and Photoperiodic Signaling in Transcriptional Nuclear Foci. Dev Cell 35(3): 311-321.
  6. Huang, H., Alvarez, S., Bindbeutel, R., Shen, Z., Naldrett, M. J., Evans, B. S., Briggs, S. P., Hicks, L. M., Kay, S. A. and Nusinow, D. A. (2016). Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry. Mol Cell Proteomics 15(1): 201-217.
  7. Waadt, R., Manalansan, B., Rauniyar, N., Munemasa, S., Booker, M. A., Brandt, B., Waadt, C., Nusinow, D. A., Kay, S. A., Kunz, H. H., Schumacher, K., DeLong, A., Yates, J. R., 3rd and Schroeder, J. I. (2015). Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses. Plant Physiol 169(1): 760-779.
  8. Chow, B. Y., Helfer, A., Nusinow, D. A. and Kay, S. A. (2012). ELF3 recruitment to the PRR9 promoter requires other Evening Complex members in the Arabidopsis circadian clock. Plant Signal Behav 7(2): 170-173.
  9. Nusinow, D. A., Helfer, A., Hamilton, E. E., King, J. J., Imaizumi, T., Schultz, T. F., Farre, E. M. and Kay, S. A. (2011). The ELF4-ELF3-LUX complex links the circadian clock to diurnal control of hypocotyl growth. Nature 475(7356): 398-402.
  10. Helfer, A., Nusinow, D. A., Chow, B. Y., Gehrke, A. R., Bulyk, M. L. and Kay, S. A. (2011). LUX ARRHYTHMO encodes a nighttime repressor of circadian gene expression in the Arabidopsis core clock. Curr Biol 21(2): 126-133.
  11. Zhang, E. E., Liu, Y., Dentin, R., Pongsawakul, P. Y., Liu, A. C., Hirota, T., Nusinow, D. A., Sun, X., Landais, S., Kodama, Y., Brenner, D. A., Montminy, M. and Kay, S. A. (2010). Cryptochrome mediates circadian regulation of cAMP signaling and hepatic gluconeogenesis. Nat Med 16(10): 1152-1156.
  12. Chang, E. Y., Ferreira, H., Somers, J., Nusinow, D. A., Owen-Hughes, T. and Narlikar, G. J. (2008). MacroH2A allows ATP-dependent chromatin remodeling by SWI/SNF and ACF complexes but specifically reduces recruitment of SWI/SNF. Biochemistry 47(51): 13726-13732.
  13. Sawa, M., Nusinow, D. A., Kay, S. A. and Imaizumi, T. (2007). FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis. Science 318(5848): 261-265.
  14. Nusinow, D. A., Sharp, J. A., Morris, A., Salas, S., Plath, K. and Panning, B. (2007). The histone domain of macroH2A1 contains several dispersed elements that are each sufficient to direct enrichment on the inactive X chromosome. J Mol Biol 371(1): 11-18.
  15. Nusinow, D. A., Hernandez-Munoz, I., Fazzio, T. G., Shah, G. M., Kraus, W. L. and Panning, B. (2007). Poly(ADP-ribose) polymerase 1 is inhibited by a histone H2A variant, MacroH2A, and contributes to silencing of the inactive X chromosome. J Biol Chem 282(17): 12851-12859.
  16. Chu, F., Nusinow, D. A., Chalkley, R. J., Plath, K., Panning, B. and Burlingame, A. L. (2006). Mapping post-translational modifications of the histone variant MacroH2A1 using tandem mass spectrometry. Mol Cell Proteomics 5(1): 194-203.
  17. de la Cruz, C. C., Fang, J., Plath, K., Worringer, K. A., Nusinow, D. A., Zhang, Y. and Panning, B. (2005). Developmental regulation of Suz 12 localization. Chromosoma 114(3): 183-192.
  18. Hernandez-Munoz, I., Lund, A. H., van der Stoop, P., Boutsma, E., Muijrers, I., Verhoeven, E., Nusinow, D. A., Panning, B., Marahrens, Y. and van Lohuizen, M. (2005). Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase. Proc Natl Acad Sci U S A 102(21): 7635-7640.
  19. Nusinow, D. A. and Panning, B. (2005). Recognition and modification of seX chromosomes. Curr Opin Genet Dev 15(2): 206-213.
  20. Plath, K., Mlynarczyk-Evans, S., Nusinow, D. A. and Panning, B. (2002). Xist RNA and the mechanism of X chromosome inactivation. Annu Rev Genet 36: 233-278.
  21. Choi, W. S., Yan, M., Nusinow, D. and Gralla, J. D. (2002). In vitro transcription and start site selection in Schizosaccharomyces pombe. J Mol Biol 319(5): 1005-1013.
1 Protocol published
Tandem Purification of His6-3x FLAG Tagged Proteins for Mass Spectrometry from Arabidopsis
Authors:  He Huang and Dmitri Anton Nusinow, date: 12/05/2016, view: 2406, Q&A: 0
Tandem affinity purification is a powerful method to identify protein complexes that function in association with a known gene of interest. This protocol describes a methodology to capture proteins tagged with His6-3x FLAG explicitly for ...