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Coauthors
Yonglun Luo Department of Biomedicine, Aarhus University, Denmark
1 protocol

Anders Nielsen Department of Biomedicine, Aarhus University, Denmark
1 protocol

Kristopher Jensen Department of Biomedicine, Aarhus University, Denmark
1 protocol

Johan Vad-Nielsen Department of Biomedicine, Aarhus University, Denmark
1 protocol

Lin Lin
  • Department of Biomedicine, Aarhus University, Denmark
Contributions
  • 1 Author merit

Education

Ph.D in Medicine, Department of Biomedicine, Aarhus University, Denmark, 2011

Current Position

Post doc in Department of Biomedicine, Aarhus University, Denmark

Publications

  1. Lin, L., Vad-Nielsen, J. and Luo, Y. (2016). CRISPR-mediated multiplexed genetic manipulation. Oncotarget. [Epub ahead of print]
  2. Zhou, Y., Liu, Y., Hussmann, D., Brogger, P., Al-Saaidi, R. A., Tan, S., Lin, L., Petersen, T. S., Zhou, G. Q., Bross, P., Aagaard, L., Klein, T., Ronn, S. G., Pedersen, H. D., Bolund, L., Nielsen, A. L., Sorensen, C. B. and Luo, Y. (2016). Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system. Cell Mol Life Sci 73(13): 2543-2563.
  3. Vad-Nielsen, J., Lin, L., Bolund, L., Nielsen, A. L. and Luo, Y. (2016). Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes. Cell Mol Life Sci 73(22): 4315-4325.
  4. Liu, T., Dou, H., Xiang, X., Li, L., Li, Y., Lin, L., Pang, X., Zhang, Y., Chen, Y., Luan, J., Xu, Y., Yang, Z., Yang, W., Liu, H., Li, F., Wang, H., Yang, H., Bolund, L., Vajta, G. and Du, Y. (2015). Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos. Cell Reprogram 17(6): 463-471.
  5. Lin, L., Bolund, L. and Luo, Y. (2016). Towards Personalized Regenerative Cell Therapy: Mesenchymal Stem Cells Derived from Human Induced Pluripotent Stem Cells. Curr Stem Cell Res Ther 11(2): 122-130.
  6. Lin, L., Yuan, J., Sander, B. and Golas, M. M. (2015). In Vitro Differentiation of Human Neural Progenitor Cells Into Striatal GABAergic Neurons. Stem Cells Transl Med 4(7): 775-788.
  7. Luo, Y., Lin, L., Golas, M., Sørensen, C. and Bolund, L. (2015). Targeted Porcine Genome Engineering with TALENs. In: Li, X. Q., Donnelly, D. J. and Jensen, T. G. (eds). Somatic Genome Manipulation: Advances, Methods and Applications.
  8. Lin, L., Luo, Y., Sorensen, P., Praetorius, H., Vajta, G., Callesen, H., Pribenszky, C., Bolund, L. and Kristensen, T. N. (2014). Effects of high hydrostatic pressure on genomic expression profiling of porcine parthenogenetic activated and cloned embryos. Reprod Fertil Dev 26(3): 469-484.
  9. Luo, Y., Lin, L., Bolund, L. and Sorensen, C. B. (2014). Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning. Biotechniques 56(5): 263-268.
  10. Zhang, P., Liu, P., Dou, H., Chen, L., Chen, L., Lin, L., Tan, P., Vajta, G., Gao, J., Du, Y. and Ma, R. Z. (2013). Handmade cloned transgenic sheep rich in omega-3 Fatty acids. PLoS One 8(2): e55941.
  11. Fan, N., Chen, J., Shang, Z., Dou, H., Ji, G., Zou, Q., Wu, L., He, L., Wang, F., Liu, K., Liu, N., Han, J., Zhou, Q., Pan, D., Yang, D., Zhao, B., Ouyang, Z., Liu, Z., Zhao, Y., Lin, L., Zhong, C., Wang, Q., Wang, S., Xu, Y., Luan, J., Liang, Y., Yang, Z., Li, J., Lu, C., Vajta, G., Li, Z., Ouyang, H., Wang, H., Wang, Y., Yang, Y., Liu, Z., Wei, H., Luan, Z., Esteban, M. A., Deng, H., Yang, H., Pei, D., Li, N., Pei, G., Liu, L., Du, Y., Xiao, L. and Lai, L. (2013). Piglets cloned from induced pluripotent stem cells. Cell Res 23(1): 162-166.
  12. Liu, Y., Ostrup, O., Li, J., Vajta, G., Lin, L., Kragh, P. M., Purup, S., Hyttel, P. and Callesen, H. (2012). Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin. Zygote 20(1): 61-66.
  13. Luo, Y., Lin, L., Bolund, L., Jensen, T. G. and Sorensen, C. B. (2012). Genetically modified pigs for biomedical research. J Inherit Metab Dis 35(4): 695-713.
  14. Pribenszky, C., Lin, L., Du, Y., Losonczi, E., Dinnyes, A. and Vajta, G. (2012). Controlled stress improves oocyte performance--cell preconditioning in assisted reproduction. Reprod Domest Anim 47 Suppl 4: 197-206.
  15. Gao, F., Li, S., Lin, L., Li, J., Luo, Y., Zhang, X., Nielsen, A. L. and Bolund, L. (2011). DNA methylation in peripheral blood cells of pigs cloned by somatic cell nuclear transfer. Cell Reprogram 13(4): 307-314.
  16. Gao, F., Luo, Y., Li, S., Li, J., Lin, L., Nielsen, A. L., Sorensen, C. B., Vajta, G., Wang, J., Zhang, X., Du, Y., Yang, H. and Bolund, L. (2011). Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls. PLoS One 6(10): e25901.
  17. Jakobsen, J. E., Li, J., Kragh, P. M., Moldt, B., Lin, L., Liu, Y., Schmidt, M., Winther, K. D., Schyth, B. D., Holm, I. E., Vajta, G., Bolund, L., Callesen, H., Jorgensen, A. L., Nielsen, A. L. and Mikkelsen, J. G. (2011). Pig transgenesis by Sleeping Beauty DNA transposition. Transgenic Res 20(3): 533-545.
  18. Schmidt, M., Winter, K. D., Dantzer, V., Li, J., Kragh, P. M., Du, Y., Lin, L., Liu, Y., Vajta, G., Sangild, P. T., Callesen, H. and Agerholm, J. S. (2011). Maternal endometrial oedema may increase perinatal mortality of cloned and transgenic piglets. Reprod Fertil Dev 23(5): 645-653.
  19. Lin, L., Yu, Y., Zhang, X., Yang, H., Bolund, L., Callesen, H. and Vajta, G. (2011). Aseptic minimum volume vitrification technique for porcine parthenogenetically activated blastocyst. Cryo Letters 32(1): 62-68.
  20. Luo, Y., Li, J., Liu, Y., Lin, L., Du, Y., Li, S., Yang, H., Vajta, G., Callesen, H., Bolund, L. and Sorensen, C. B. (2011). High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer. Transgenic Res 20(5): 975-988.
  21. Pribenszky, C., Vajta, G., Molnar, M., Du, Y., Lin, L., Bolund, L. and Yovich, J. (2010). Stress for stress tolerance? A fundamentally new approach in mammalian embryology. Biol Reprod 83(5): 690-697.
  22. Schmidt, M., Kragh, P. M., Li, J., Du, Y., Lin, L., Liu, Y., Bogh, I. B., Winther, K. D., Vajta, G. and Callesen, H. (2010). Pregnancies and piglets from large white sow recipients after two transfer methods of cloned and transgenic embryos of different pig breeds. Theriogenology 74(7): 1233-1240.
  23. Lin, L., Pribenszky, C., Molnar, M., Kragh, P. M., Du, Y., Zhang, X., Yang, H., Bolund, L., Callesen, H., Machaty, Z. and Vajta, G. (2010). High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activation. Cell Reprogram 12(4): 475-480.
  24. Su, Y., Lin, L., Tian, G., Chen, C., Liu, T., Xu, X., Qi, X., Zhang, X. and Yang, H. (2009). Preparing a re-sequencing DNA library of 2 cancer candidate genes using the ligation-by-amplification protocol by two PCR reactions. Sci China C Life Sci 52(5): 483-491.
  25. Kragh, P. M., Nielsen, A. L., Li, J., Du, Y., Lin, L., Schmidt, M., Bogh, I. B., Holm, I. E., Jakobsen, J. E., Johansen, M. G., Purup, S., Bolund, L., Vajta, G. and Jorgensen, A. L. (2009). Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw. Transgenic Res 18(4): 545-558.
  26. Lin, L., Du, Y., Liu, Y., Kragh, P. M., Li, J., Purup, S., Kuwayama, M., Zhang, X., Yang, H., Bolund, L. and Vajta, G. (2009). Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes. Reprod Biomed Online 18(3): 360-366.
  27. Lin, L., Kragh, P. M., Purup, S., Kuwayama, M., Du, Y., Zhang, X., Yang, H., Bolund, L., Callesen, H. and Vajta, G. (2009). Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes. Reprod Fertil Dev 21(2): 338-344.
  28. Du, Y., Lin, L., Schmidt, M., Bogh, I. B., Kragh, P. M., Sorensen, C. B., Li, J., Purup, S., Pribenszky, C., Molnar, M., Kuwayama, M., Zhang, X., Yang, H., Bolund, L. and Vajta, G. (2008). High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning Stem Cells 10(3): 325-330.
  29. Li, J., Svarcova, O., Villemoes, K., Kragh, P. M., Schmidt, M., Bogh, I. B., Zhang, Y., Du, Y., Lin, L., Purup, S., Xue, Q., Bolund, L., Yang, H., Maddox-Hyttel, P. and Vajta, G. (2008). High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. Theriogenology 70(5): 800-808.
  30. Liu, Y., Du, Y., Lin, L., Li, J., Kragh, P. M., Kuwayama, M., Bolund, L., Yang, H. and Vajta, G. (2008). Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes. Cryo Letters 29(4): 315-320.
1 Protocol published
A Golden Gate-based Protocol for Assembly of Multiplexed gRNA Expression Arrays for CRISPR/Cas9
The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) has become the most broadly used and powerful tool for genome editing. Many applications of CRISPR-Cas9 require the delivery of multiple small guide ...