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Coauthors
Roop Mallik Department of Biological Sciences, Tata Institute of Fundamental Research, India
1 protocol

Divya Pathak Department of Biological Sciences, Tata Institute of Fundamental Research, India
1 protocol

Paulomi Sanghavi Department of Biological Sciences, Tata Institute of Fundamental Research, India
1 protocol

Ashwin D’Souza Department of Biological Sciences, Tata Institute of Fundamental Research, India
1 protocol

Ashim Rai
  • Department of Biological Sciences, Tata Institute of Fundamental Research, India
Contributions
  • 1 Author merit

Education

Ph.D in Biology, Tata Institute of Fundamental Research (TIFR), Mumbai, 2016

Current Position

Postdoctoral fellow in Prof. Sivaraj Sivaramakrishnan’s lab at the University of Minnesota

Publications

  1. Rai, A., Pathak, D., Thakur, S., Singh, S., Dubey, A. K. and Mallik, R. (2016). Dynein Clusters into Lipid Microdomains on Phagosomes to Drive Rapid Transport toward Lysosomes. Cell 164(4): 722-734.
  2. Chanduri, M., Rai, A., Malla, A. B., Wu, M., Fiedler, D., Mallik, R. and Bhandari, R. (2016). Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 473(19): 3031-3047.
  3. Barak, P., Rai, A., Dubey, A. K., Rai, P. and Mallik, R. (2014). Reconstitution of microtubule-dependent organelle transport. Methods Enzymol 540: 231-248.
  4. Rai, A. K., Rai, A., Ramaiya, A. J., Jha, R. and Mallik, R. (2013). Molecular adaptations allow dynein to generate large collective forces inside cells. Cell 152(1-2): 172-182.
  5. Barak, P., Rai, A., Rai, P. and Mallik, R. (2013). Quantitative optical trapping on single organelles in cell extract. Nat Methods 10(1): 68-70.
  6. Mallik, R., Rai, A. K., Barak, P., Rai, A. and Kunwar, A. (2013). Teamwork in microtubule motors. Trends Cell Biol 23(11): 575-582.
1 Protocol published
Isolation of Latex Bead Phagosomes from Dictyostelium for in vitro Functional Assays
Authors:  Ashwin D’Souza, Paulomi Sanghavi, Ashim Rai, Divya Pathak and Roop Mallik, date: 12/05/2016, view: 1935, Q&A: 0
We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven ...