Murugappan Sathappa Broad Institute
1 protocol

Nathan N. Alder
  • Department of Molecular and Cell Biology, University of Connecticut, USA
  • 1 Author merit


Ph.D. in University of California, Davis

Current Position

Associate professor, Department of Molecular and Cell Biology, University of Connecticut

Publications (since 2005)

  1. Sathappa, M. and Alder, N. N. (2016). The ionization properties of cardiolipin and its variants in model bilayers. Biochim Biophys Acta 1858(6): 1362-1372.

  2. Lee, K. K., Imaizumi, N., Chamberland, S. R., Alder, N. N. and Boelsterli, U. A. (2015). Targeting mitochondria with methylene blue protects mice against acetaminophen-induced liver injury. Hepatology 61(1): 326-336.
  3. Malhotra, K. and Alder, N. N. (2014). Advances in the use of nanoscale bilayers to study membrane protein structure and function. Biotechnol Genet Eng Rev 30(1-2): 79-93.
  4. Hwang, M. S., Schwall, C. T., Pazarentzos, E., Datler, C., Alder, N. N. and Grimm, S. (2014). Mitochondrial Ca(2+) influx targets cardiolipin to disintegrate respiratory chain complex II for cell death induction. Cell Death Differ 21(11): 1733-1745.
  5. Baile, M. G., Sathappa, M., Lu, Y. W., Pryce, E., Whited, K., McCaffery, J. M., Han, X., Alder, N. N. and Claypool, S. M. (2014). Unremodeled and remodeled cardiolipin are functionally indistinguishable in yeast. J Biol Chem 289(3): 1768-1778.
  6. Schwall, C. T. and Alder, N. N. (2013). Site-specific fluorescent probe labeling of mitochondrial membrane proteins. Methods Mol Biol 1033: 103-120.
  7. Lee, K. K., Fujimoto, K., Zhang, C., Schwall, C. T., Alder, N. N., Pinkert, C. A., Krueger, W., Rasmussen, T. and Boelsterli, U. A. (2013). Isoniazid-induced cell death is precipitated by underlying mitochondrial complex I dysfunction in mouse hepatocytes. Free Radic Biol Med 65: 584-594.
  8. Malhotra, K., Sathappa, M., Landin, J. S., Johnson, A. E. and Alder, N. N. (2013). Structural changes in the mitochondrial Tim23 channel are coupled to the proton-motive force. Nat Struct Mol Biol 20(8): 965-972.
  9. Long, A. R., O'Brien, C. C., Malhotra, K., Schwall, C. T., Albert, A. D., Watts, A. and Alder, N. N. (2013). A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs. BMC Biotechnol 13: 41.
  10. Long, A. R., O'Brien, C. C. and Alder, N. N. (2012). The cell-free integration of a polytopic mitochondrial membrane protein into liposomes occurs cotranslationally and in a lipid-dependent manner. PLoS One 7(9): e46332.
  11. Schwall, C. T., Greenwood, V. L. and Alder, N. N. (2012). The stability and activity of respiratory Complex II is cardiolipin-dependent. Biochim Biophys Acta 1817(9): 1588-1596.
  12. Ranaghan, M. J., Schwall, C. T., Alder, N. N. and Birge, R. R. (2011). Green proteorhodopsin reconstituted into nanoscale phospholipid bilayers (nanodiscs) as photoactive monomers. J Am Chem Soc 133(45): 18318-18327.
  13. Jha, S., Patil, S. M., Gibson, J., Nelson, C. E., Alder, N. N. and Alexandrescu, A. T. (2011). Mechanism of amylin fibrillization enhancement by heparin. J Biol Chem 286(26): 22894-22904.
  14. Alder, N. N., Jensen, R. E. and Johnson, A. E. (2008). Fluorescence mapping of mitochondrial TIM23 complex reveals a water-facing, substrate-interacting helix surface. Cell 134(3): 439-450.
  15. Alder, N. N., Sutherland, J., Buhring, A. I., Jensen, R. E. and Johnson, A. E. (2008). Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits. Mol Biol Cell 19(1): 159-170.
  16. Davis, A. J., Alder, N. N., Jensen, R. E. and Johnson, A. E. (2007). The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import. Mol Biol Cell 18(2): 475-486.
  17. James, J. J., Alder, N. N., Muhling, K. H., Lauchli, A. E., Shackel, K. A., Donovan, L. A. and Richards, J. H. (2006). High apoplastic solute concentrations in leaves alter water relations of the halophytic shrub, Sarcobatus vermiculatus. J Exp Bot 57(1): 139-147.
  18. Alder, N. N., Shen, Y., Brodsky, J. L., Hendershot, L. M. and Johnson, A. E. (2005). The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum. J Cell Biol 168(3): 389-399.
1 Protocol published
Ionization Properties of Phospholipids Determined by Zeta Potential Measurements
Authors:  Murugappan Sathappa and Nathan N. Alder, date: 11/20/2016, view: 2086, Q&A: 0
Biological membranes are vital for diverse cellular functions such as maintaining cell and organelle structure, selective permeability, active transport, and signaling. The surface charge of the membrane bilayer plays a critical role in these myriad ...