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Coauthors
Cammie Lesser Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, USA
1 protocol

Analise Z Reeves
  • Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, USA
  • 1 Author merit

Education

Ph.D. in Department of Microbiology and Molecular Genetics, Emory University, Atlanta, GA, 2010

Current position

Postdoctoral Fellow, Massachusetts General Hospital, Harvard Medical School

Publications

  1. Du, J., Reeves, A. Z., Klein, J. A., Twedt, D. J., Knodler, L. A. and Lesser, C. F. (2016). The type III secretion system apparatus determines the intracellular niche of bacterial pathogens. Proc Natl Acad Sci U S A 113(17): 4794-4799.

  2. Reeves, A. Z., Spears, W. E., Du, J., Tan, K. Y., Wagers, A. J. and Lesser, C. F. (2015). Engineering Escherichia coli into a protein delivery system for mammalian cells. ACS Synth Biol 4(5): 644-654.

  3. Reeves, A. Z., Costa, S. and Lesser, C. F. (2016). Molecular approaches to identifying type 3 secreted effectors, a Shigella case study. In: Picking, W. D. and Picking, W. L. (eds). Shigella: Molecular and Cellular Biology, Haverhill, UK, Caister Academic Press.

  4. Reeves, A. Z., Campbell, P. J., Willby, M. J. and Posey, J. E. (2015). Disparities in capreomycin resistance levels associated with the rrs A1401G mutation in clinical isolates of Mycobacterium tuberculosis. Antimicrob Agents Chemother 59(1): 444-449.

  5. Baldwin, P. R., Reeves, A. Z., Powell, K. R., Napier, R. J., Swimm, A. I., Sun, A., Giesler, K., Bommarius, B., Shinnick, T. M., Snyder, J. P., Liotta, D. C. and Kalman, D. (2015). Monocarbonyl analogs of curcumin inhibit growth of antibiotic sensitive and resistant strains of Mycobacterium tuberculosis. Eur J Med Chem 92: 693-699.

  6. Reeves, A. Z., Campbell, P. J., Sultana, R., Malik, S., Murray, M., Plikaytis, B. B., Shinnick, T. M. and Posey, J. E. (2013). Aminoglycoside cross-resistance in Mycobacterium tuberculosis due to mutations in the 5' untranslated region of whiB7. Antimicrob Agents Chemother 57(4): 1857-1865.

  7. Napier, R. J., Rafi, W., Cheruvu, M., Powell, K. R., Zaunbrecher, M. A., Bornmann, W., Salgame, P., Shinnick, T. M. and Kalman, D. (2011). Imatinib-sensitive tyrosine kinases regulate mycobacterial pathogenesis and represent therapeutic targets against tuberculosis. Cell Host Microbe 10(5): 475-485.

  8. Zaunbrecher, M. A., Sikes, R. D., Jr., Metchock, B., Shinnick, T. M. and Posey, J. E. (2009). Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 106(47): 20004-20009.

  9. Mann, E., Zaunbrecher, M. A., Hitz, K. and Churchward, G. (2008). The 52nd Annual Wind River Conference On Prokaryotic Biology--2008. J Bacteriol 190(24): 7871-7875.

1 Protocol published
Transfer of Large Contiguous DNA Fragments onto a Low Copy Plasmid or into the Bacterial Chromosome
Authors:  Analise Z Reeves and Cammie F Lesser, date: 11/20/2016, view: 2488, Q&A: 0
Bacterial pathogenicity islands and other contiguous operons can be difficult to clone using conventional methods due to their large size. Here we describe a robust 3-step method to transfer large defined fragments of DNA from virulence plasmids or ...