Toshihiro Kawasaki Genetic Strains Research Center, National Institute of Genetics, and Department of Genetics, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), Japan
1 protocol

Noriyoshi Sakai
  • Genetic Strains Research Center, National Institute of Genetics, and Department of Genetics, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), Japan
  • 1 Author merit


Ph.D in Biology, Kanazawa University, 1989.

Current position

Associate professor in Genetic Strains Research Center, National Institute of Genetics, and School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Japan.


  1. Kawasaki, T., Siegfried, K. R. and Sakai, N. (2016). Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Development 143(4): 566-574.

  2. Higaki, S., Shimada, M., Koyama, Y., Fujioka, Y., Sakai, N. and Takada, T. (2015). Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883). In Vitro Cell Dev Biol Anim 51(8): 763-768.

  3. Saito, K., Sakai, C., Kawasaki, T. and Sakai, N. (2014). Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Dev Dyn 243(11): 1448-1456.

  4. Nishimura, T., Herpin, A., Kimura, T., Hara, I., Kawasaki, T., Nakamura, S., Yamamoto, Y., Saito, T. L., Yoshimura, J., Morishita, S., Tsukahara, T., Kobayashi, S., Naruse, K., Shigenobu, S., Sakai, N., Schartl, M. and Tanaka, M. (2014). Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation. Development 141(17): 3363-3369.

  5. Shinya, M., Kobayashi, K., Masuda, A., Tokumoto, M., Ozaki, Y., Saito, K., Kawasaki, T., Sado, Y. and Sakai, N. (2013). Properties of gene knockdown system by vector-based siRNA in zebrafish. Dev Growth Differ 55(9): 755-765.

  6. Higaki, S., Koyama, Y., Shirai, E., Yokota, T., Fujioka, Y., Sakai, N. and Takada, T. (2013). Establishment of testicular and ovarian cell lines from Honmoroko (Gnathopogon caerulescens). Fish Physiol Biochem 39(3): 701-711.

  7. Higaki, S., Koyama, Y., Shimada, M., Ono, Y., Tooyama, I., Fujioka, Y., Sakai, N., Ikeuchi, T. and Takada, T. (2013). Response to fish specific reproductive hormones and endocrine disrupting chemicals of a Sertoli cell line expressing endogenous receptors from an endemic cyprinid Gnathopogon caerulescens. Gen Comp Endocrinol 191: 65-73.

  8. Kawasaki, T., Saito, K., Sakai, C., Shinya, M. and Sakai, N. (2012). Production of zebrafish offspring from cultured spermatogonial stem cells. Genes Cells 17(4): 316-325.

  9. Shinya, M. and Sakai, N. (2011). Generation of highly homogeneous strains of zebrafish through full sib-pair mating. G3 (Bethesda) 1(5): 377-386.

  10. Saito, K., Siegfried, K. R., Nusslein-Volhard, C. and Sakai, N. (2011). Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants. Dev Dyn 240(7): 1779-1792.

  11. Ozaki, Y., Saito, K., Shinya, M., Kawasaki, T. and Sakai, N. (2011). Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. Gene Expr Patterns 11(5-6): 309-315.

  12. Torihara, H., Uechi, T., Chakraborty, A., Shinya, M., Sakai, N. and Kenmochi, N. (2011). Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia. Br J Haematol 152(5): 648-654.

  13. Kawasaki, T., Saito, K., Shinya, M., Olsen, L. C. and Sakai, N. (2010). Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in Zebrafish (Danio rerio). Biol Reprod 83(4): 533-539.

  14. Kawasaki, T., Saito, K., Mitsui, K., Ikawa, M., Yamashita, M., Taniguchi, Y., Takeda, S., Mitani, K. and Sakai, N. (2009). Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. Zebrafish 6(3): 253-258.

  15. Hashiguchi, M., Shinya, M., Tokumoto, M. and Sakai, N. (2008). Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines. Dev Biol 321(2): 387-396.

  16. Kimura, T., Shimada, A., Sakai, N., Mitani, H., Naruse, K., Takeda, H., Inoko, H., Tamiya, G. and Shinya, M. (2007). Genetic analysis of craniofacial traits in the medaka. Genetics 177(4): 2379-2388.

  17. Sakai, N. (2006). In vitro male germ cell cultures of zebrafish. Methods 39(3): 239-245.

1 Protocol published
Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 1541, Q&A: 0
Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These ...