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Coauthors
Venu Vandavasi Biology and Soft Matter Division, Oak Ridge National Laboratory, USA
1 protocol

Hugh O’ Neill
  • Biology and Soft Matter Division, Oak Ridge National Laboratory, USA
  • 1 Author merit

Education

Ph.D in Department of Biochemistry, University College Dublin, 1997

Current position

Senior Staff scientist, Institutional Biosafety Committee, ORNL

Publications

  1. Vandavasi, V. G., Putnam, D. K., Zhang, Q., Petridis, L., Heller, W. T., Nixon, B. T., Haigler, C. H., Kalluri, U., Coates, L., Langan, P., Smith, J. C., Meiler, J. and O'Neill, H. (2016). A Structural Study of CESA1 Catalytic Domain of Arabidopsis Cellulose Synthesis Complex: Evidence for CESA Trimers. Plant Physiol 170(1): 123-135.

  2. Stingaciu, L. R., O'Neill, H., Liberton, M., Urban, V. S., Pakrasi, H. B. and Ohl, M. (2016). Revealing the Dynamics of Thylakoid Membranes in Living Cyanobacterial Cells. Sci Rep 6: 19627.

  3. Rai, D. K., Sharma, V. K., Anunciado, D., O'Neill, H., Mamontov, E., Urban, V., Heller, W. T. and Qian, S. (2016). Neutron Scattering Studies of the Interplay of Amyloid beta Peptide(1-40) and An Anionic Lipid 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol. Sci Rep 6: 30983.

  4. Nixon, B. T., Mansouri, K., Singh, A., Du, J., Davis, J. K., Lee, J. G., Slabaugh, E., Vandavasi, V. G., O'Neill, H., Roberts, E. M., Roberts, A. W., Yingling, Y. G. and Haigler, C. H. (2016). Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex. Sci Rep 6: 28696.

  5. Nixon, B. T., Mansouri, K., Singh, A., Du, J., Davis, J. K., Lee, J. G., Slabaugh, E., Vandavasi, V. G., O'Neill, H., Roberts, E. M., Roberts, A. W., Yingling, Y. G. and Haigler, C. H. (2016). Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex. Sci Rep 6: 28696.

  6. O'Neill, H., Shah, R., Evans, B. R., He, J., Pingali, S. V., Chundawat, S. P., Jones, A. D., Langan, P., Davison, B. H. and Urban, V. (2015). Production of bacterial cellulose with controlled deuterium-hydrogen substitution for neutron scattering studies. Methods Enzymol 565: 123-146.

  7. Jiang, J., Zhang, H., Lu, X., Lu, Y., Cuneo, M. J., O'Neill, H. M., Urban, V., Lo, C. S. and Blankenship, R. E. (2015). Oligomerization state and pigment binding strength of the peridinin-Chl a-protein. FEBS Lett 589(19 Pt B): 2713-2719.

  8. Anunciado, D., Rai, D. K., Qian, S., Urban, V. and O'Neill, H. (2015). Small-angle neutron scattering reveals the assembly of alpha-synuclein in lipid membranes. Biochim Biophys Acta 1854(12): 1881-1889.

  9. Sharma, V. K., Mamontov, E., Anunciado, D. B., O'Neill, H. and Urban, V. (2015). Nanoscopic dynamics of phospholipid in unilamellar vesicles: effect of gel to fluid phase transition. J Phys Chem B 119(12): 4460-4470.

  10. Hayes, D. G., Gomez del Rio, J. A., Ye, R., Urban, V. S., Pingali, S. V. and O'Neill, H. M. (2015). Effect of protein incorporation on the nanostructure of the bicontinuous microemulsion phase of Winsor-III systems: a small-angle neutron scattering study. Langmuir 31(6): 1901-1910.

  11. Evans, B. R., Bali, G., Foston, M., Ragauskas, A. J., O'Neill, H. M., Shah, R., McGaughey, J., Reeves, D., Rempe, C. S. and Davison, B. H. (2015). Production of deuterated switchgrass by hydroponic cultivation. Planta 242(1): 215-222.

  12. Petridis, L., O'Neill, H. M., Johnsen, M., Fan, B., Schulz, R., Mamontov, E., Maranas, J., Langan, P. and Smith, J. C. (2014). Hydration control of the mechanical and dynamical properties of cellulose. Biomacromolecules 15(11): 4152-4159.

1 Protocol published
Heterologous Expression and Purification of Catalytic Domain of CESA1 from Arabidopsis thaliana
Authors:  Venu Gopal Vandavasi and Hugh O’ Neill, date: 10/20/2016, view: 1830, Q&A: 0
Heterologous expression of plant cellulose synthase (CESA) and its purification has remained a challenge for decades impeding detailed biophysical, biochemical and structural characterization of this key enzyme. An in-depth knowledge of structure ...