Rachel Caspi Laboratory of Immunology, National Eye Institute, NIH, USA
1 protocol

Reiko Horai Laboratory of Immunology, National Eye Institute, NIH, USA
1 protocol

Phyllis Silver Laboratory of Immunology, National Eye Institute, NIH, USA
1 protocol

Patricia Dillenburg-Pilla Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil
1 protocol

Carlos R. Zárate-Bladés
  • Laboratory of Immunoregulation, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianopolis, Brazil
  • 1 Author merit


Ph.D in University of São Paulo, Program of Basic and Applied Immunology. Ribeirão Preto, São Paulo, Brazil, 2006

Current position

Assistant Professor, Dept of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianopolis, SC, Brazil


  1. Zarate-Blades, C. R., Horai, R. and Caspi, R. R. (2016). Regulation of Autoimmunity by the Microbiome. DNA Cell Biol.

  2. Auchtung, T. A., Holder, M. E., Gesell, J. R., Ajami, N. J., Duarte, R. T., Itoh, K., Caspi, R. R., Petrosino, J. F., Horai, R. and Zarate-Blades, C. R. (2016). Complete Genome Sequence of Turicibacter sp. Strain H121, Isolated from the Feces of a Contaminated Germ-Free Mouse. Genome Announc 4(2).

  3. Horai, R., Zarate-Blades, C. R., Dillenburg-Pilla, P., Chen, J., Kielczewski, J. L., Silver, P. B., Jittayasothorn, Y., Chan, C. C., Yamane, H., Honda, K. and Caspi, R. R. (2015). Microbiota-Dependent Activation of an Autoreactive T Cell Receptor Provokes Autoimmunity in an Immunologically Privileged Site. Immunity 43(2): 343-353.

  4. Rodrigues, R. F., Zarate-Blades, C. R., Rios, W. M., Soares, L. S., Souza, P. R., Brandao, I. T., Masson, A. P., Arnoldi, F. G., Ramos, S. G., Letourneur, F., Jacques, S., Cagnard, N., Chiocchia, G. and Silva, C. L. (2015). Synergy of chemotherapy and immunotherapy revealed by a genome-scale analysis of murine tuberculosis. J Antimicrob Chemother 70(6): 1774-1783.

  5. Dillenburg-Pilla, P., Patel, V., Mikelis, C. M., Zarate-Blades, C. R., Doci, C. L., Amornphimoltham, P., Wang, Z., Martin, D., Leelahavanichkul, K., Dorsam, R. T., Masedunskas, A., Weigert, R., Molinolo, A. A. and Gutkind, J. S. (2015). SDF-1/CXCL12 induces directional cell migration and spontaneous metastasis via a CXCR4/Galphai/mTORC1 axis. FASEB J 29(3): 1056-1068.

  6. Doimo, N. T., Zarate-Blades, C. R., Rodrigues, R. F., Tefe-Silva, C., Trotte, M. N., Souza, P. R., Soares, L. S., Rios, W. M., Floriano, E. M., Brandao, I. T., Masson, A. P., Coelho, V., Ramos, S. G. and Silva, C. L. (2014). Immunotherapy of tuberculosis with Mycobacterium leprae Hsp65 as a DNA vaccine triggers cross-reactive antibodies against mammalian Hsp60 but not pathological autoimmunity. Hum Vaccin Immunother 10(5): 1238-1243.

  7. Zarate-Blades, C. R., Rodrigues, R. F., Souza, P. R., Rios, W. M., Soares, L. S., Rosada, R. S., Brandao, I. T., Masson, A. P., Floriano, E. M., Ramos, S. G. and Silva, C. L. (2013). Evaluation of the overall IFN-gamma and IL-17 pro-inflammatory responses after DNA therapy of tuberculosis. Hum Vaccin Immunother 9(5): 1093-1103.

  8. Zhou, R., Horai, R., Silver, P. B., Mattapallil, M. J., Zarate-Blades, C. R., Chong, W. P., Chen, J., Rigden, R. C., Villasmil, R. and Caspi, R. R. (2012). The living eye "disarms" uncommitted autoreactive T cells by converting them to Foxp3(+) regulatory cells following local antigen recognition. J Immunol 188(4): 1742-1750.

  9. Zarate-Blades, C. R., Silva, C. L. and Passos, G. A. (2011). The impact of transcriptomics on the fight against tuberculosis: focus on biomarkers, BCG vaccination, and immunotherapy. Clin Dev Immunol 2011: 192630.

  10. dos Santos, S. A., Zarate-Blades, C. R., de Sa Galetti, F. C., Brandao, I. T., Masson, A. P., Soares, E. G., Araujo, A. P. and Silva, C. L. (2010). A subunit vaccine based on biodegradable microspheres carrying rHsp65 protein and KLK protects BALB/c mice against tuberculosis infection. Hum Vaccin 6(12): 1047-1053.

  11. Zorzenon dos Santos, R. M., Amador, A., de Souza, W. V., de Albuquerque, M. F., Ponce Dawson, S., Ruffino-Netto, A., Zarate-Blades, C. R. and Silva, C. L. (2010). A dynamic analysis of tuberculosis dissemination to improve control and surveillance. PLoS One 5(11): e14140.

  12. Rosada, R. S., Moreira, A. P., Frantz, F. G., Puri, R. K., Rahman, A., Standiford, T. J., Zarate-Blades, C. R., Silva, C. L. and Hogaboam, C. M. (2010). Therapeutic efficacy of Cintredekin Besudotox (IL13-PE38QQR) in murine lung fibrosis is unaffected by immunity to Pseudomonas aeruginosa exotoxin A. PLoS One 5(1): e8721.

  13. Lima, D. S., Zárate-Bladés, C. R., Souza, P. R. M., Trombone, A. P., Santos, R. R. Jr., Brandão, I. T., Masson, A. P., Bonato, V. L. D., Coelho-Castelo, A. A. M., Sartori, A., Vendramini, M., Soares, E. G., Silva, C. L. and Coelho, V. (2009). No evidence of pathological autoimmunity following Mycobacterium leprae heat-shock protein 65-DNA vaccination in mice. European Journal of Inflammation. 2009; 7(2):77-85

  14. Zarate-Blades, C. R., Bonato, V. L., da Silveira, E. L., Oliveira e Paula, M., Junta, C. M., Sandrin-Garcia, P., Fachin, A. L., Mello, S. S., Cardoso, R. S., Galetti, F. C., Coelho-Castelo, A. A., Ramos, S. G., Donadi, E. A., Sakamoto-Hojo, E. T., Passos, G. A. and Silva, C. L. (2009). Comprehensive gene expression profiling in lungs of mice infected with Mycobacterium tuberculosis following DNAhsp65 immunotherapy. J Gene Med 11(1): 66-78.

  15. Marques, M. M., Junta, C. M., Zarate-Blades, C. R., Sakamoto-Hojo, E. T., Donadi, E. A. and Passos, G. A. (2009). Transcriptional response of peripheral lymphocytes to early fibrosarcoma: a model system for cancer detection based on hybridization signatures. Exp Biol Med (Maywood) 234(7): 802-812.

  16. Victora, G. D., Socorro-Silva, A., Volsi, E. C., Abdallah, K., Lima, F. D., Smith, R. B., Moyses, R. A., Zarate-Blades, C. R., Michaluart, P., Silva, C. L., Kalil, J. and Coelho, V. (2009). Immune response to vaccination with DNA-Hsp65 in a phase I clinical trial with head and neck cancer patients. Cancer Gene Ther 16(7): 598-608.

  17. Ribeiro, A. M., Bocca, A. L., Amaral, A. C., Faccioli, L. H., Galetti, F. C., Zarate-Blades, C. R., Figueiredo, F., Silva, C. L. and Felipe, M. S. (2009). DNAhsp65 vaccination induces protection in mice against Paracoccidioides brasiliensis infection. Vaccine 27(4): 606-613.

  18. Silva, C. L., Bonato, V. L., dos Santos-Junior, R. R., Zarate-Blades, C. R. and Sartori, A. (2009). Recent advances in DNA vaccines for autoimmune diseases. Expert Rev Vaccines 8(2): 239-252.

  19. Souza, P. R., Zarate-Blades, C. R., Hori, J. I., Ramos, S. G., Lima, D. S., Schneider, T., Rosada, R. S., Torre, L. G., Santana, M. H., Brandao, I. T., Masson, A. P., Coelho-Castelo, A. A., Bonato, V. L., Galetti, F. C., Goncalves, E. D., Botte, D. A., Machado, J. B. and Silva, C. L. (2008). Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis. Expert Opin Biol Ther 8(9): 1255-1264.

  20. Rosada, R. S., de la Torre, L. G., Frantz, F. G., Trombone, A. P., Zarate-Blades, C. R., Fonseca, D. M., Souza, P. R., Brandao, I. T., Masson, A. P., Soares, E. G., Ramos, S. G., Faccioli, L. H., Silva, C. L., Santana, M. H. and Coelho-Castelo, A. A. (2008). Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes. BMC Immunol 9: 38.

  21. Michaluart, P., Abdallah, K. A., Lima, F. D., Smith, R., Moyses, R. A., Coelho, V., Victora, G. D., Socorro-Silva, A., Volsi, E. C., Zarate-Blades, C. R., Ferraz, A. R., Barreto, A. K., Chammas, M. C., Gomes, R., Gebrim, E., Arakawa-Sugueno, L., Fernandes, K. P., Lotufo, P. A., Cardoso, M. R., Kalil, J. and Silva, C. L. (2008). Phase I trial of DNA-hsp65 immunotherapy for advanced squamous cell carcinoma of the head and neck. Cancer Gene Ther 15(10): 676-684.

  22. de Paula, L., Silva, C. L., Carlos, D., Matias-Peres, C., Sorgi, C. A., Soares, E. G., Souza, P. R., Blades, C. R., Galleti, F. C., Bonato, V. L., Goncalves, E. D., Silva, E. V. and Faccioli, L. H. (2007). Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs. Genet Vaccines Ther 5: 2.

  23. Zarate-Blades, C. R., Blades, N., Nascimento, M. S., da Silveira, J. F. and Umezawa, E. S. (2007). Diagnostic performance of tests based on Trypanosoma cruzi excreted-secreted antigens in an endemic area for Chagas' disease in Bolivia. Diagn Microbiol Infect Dis 57(2): 229-232.

  24. Cardoso, R. S., Junta, C. M., Macedo, C., Magalhaes, D. A., Silveira, E. L., Paula, M. O., Marques, M. M., Mello, S. S., Zarate-Blades, C. R., Nguyen, C., Houlgatte, R., Donadi, E. A., Sakamoto-Hojo, E. T. and Passos, G. A. (2006). Hybridization signatures of gamma-irradiated murine fetal thymus organ culture (FTOC) reveal modulation of genes associated with T-cell receptor V(D)J recombination and DNA repair. Mol Immunol 43(5): 464-472.

1 Protocol published
Preparation of Protein-containing Extracts from Microbiota-rich Intestinal Contents
The contribution of microbiota in regulating multiple physiological and pathological host responses has been studied intensively in recent years. Evidence suggests that commensal microbiota can directly modulate different populations of cells of the ...