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Coauthors
Kyosuke Nagata Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Japan
1 protocol
Coreviewers
Arsalan Daudi University of California
153 protocols

Stéphane Romero Ecole Polytechnique
1 protocol

Reviewer
Kenji Sugiyama
  • University of Tsukuba
Research focus
  • Biochemistry
  • Influenza virus
    biochemistry
  • 1 Author merit

Lab information

Nagata Lab

1 Protocol published
Purification and Identification of Novel Host-derived Factors for Influenza Virus Replication from Human Nuclear Extracts
Authors:  Kenji Sugiyama and Kyosuke Nagata, date: 09/20/2016, view: 2506, Q&A: 0
Recently, we identified two host cell-derived proteins as novel stimulatory factors of influenza virus RNA replication process, termed “Influenza virus REplication Factor-2 (IREF-2)”, from human nuclear extracts (NEs) by employing biochemical ...
1 Protocol reviewed
Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor
Authors:  Rajan Lamichhane, Jeffrey J. Liu, Raymond F. Pauszek III and David P. Millar, date: 06/20/2017, view: 1242, Q&A: 0
Activation of G protein-coupled receptors (GPCRs) by agonist ligands is mediated by a transition from an inactive to active receptor conformation. We describe a novel single-molecule assay that monitors activation-linked conformational transitions ...
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