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Coauthors
Juan Santiago Department of Mechanical Engineering, Stanford University, USA
1 protocol

Kentaro Kuriyama Department of Mechanical Engineering, Stanford University, USA
1 protocol

Hirofumi Shintaku
  • Department of Mechanical Engineering, Stanford University, USA
Contributions
  • 1 Author merit

Education

Ph.D., Mechanical Engineering, Kyoto University, 2006

Current position

Assistant Professor, Department of Micro Engineering, Graduate School of Engineering, Kyoto University

Publications (since 2014)

  1. Kuriyama, K.*, Shintaku, H.* and Santiago, J. G. (2015). Isotachophoresis for fractionation and recovery of cytoplasmic RNA and nucleus from single cells. Electrophoresis 36(14): 1658-1662.[*Equal contributors]
  2. Han, C. M., Katilius, E. and Santiago, J. G. (2014). Increasing hybridization rate and sensitivity of DNA microarrays using isotachophoresis. Lab Chip 14(16): 2958-2967.
  3. Shintaku, H., Nishikii, H., Marshall, L. A., Kotera, H. and Santiago, J. G. (2014). On-chip separation and analysis of RNA and DNA from single cells. Anal Chem 86(4): 1953-1957.
  4. Shintaku, H.,Kazumi, H., Hiroshi, F., Takeshi, N., Jun, M., Satoyuki, K. (2014). Measurement of Local Electric Field in Microdevices for Low-Voltage Electroporation of Adherent Cells. Microsystem Technologies, Volume 20, Issue 2, pp 303-313.
1 Protocol published
Protocol for Microfluidic System to Automate the Preparation and Fractionation of the Nucleic Acids in the Cytoplasm Versus Nuclei of Single Cells
Authors:  Kentaro Kuriyama, Hirofumi Shintaku and Juan G. Santiago, date: 06/20/2016, view: 1669, Q&A: 0
This protocol describes the extraction, fractionation, and recovery of cytoplasmic nucleic acids (e.g., cytoplasmic RNA) versus nucleic acids in the cell nucleus (including genomic DNA, gDNA) from single cells with a microfluidic system. ...