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Coauthors
Juan Santiago Department of Mechanical Engineering, Stanford University, USA
1 protocol

Hirofumi Shintaku Department of Mechanical Engineering, Stanford University, USA
1 protocol

Kentaro Kuriyama
  • Department of Mechanical Engineering, Stanford University, USA
Contributions
  • 1 Author merit

Education

M.E., Mechanical Engineering, Tokyo Institute of Technology, Japan, 2006

Current position

Mechanical Engineer, Manufacturing Engineering Division and Medical Business Unit, Life Science Business Division, Sony Corporation, Japan

Publications

  1. Kuriyama, K., Shintaku, H. and Santiago, J. G. (2015). Isotachophoresis for fractionation and recovery of cytoplasmic RNA and nucleus from single cells. Electrophoresis 36(14): 1658-1662.
1 Protocol published
Protocol for Microfluidic System to Automate the Preparation and Fractionation of the Nucleic Acids in the Cytoplasm Versus Nuclei of Single Cells
Authors:  Kentaro Kuriyama, Hirofumi Shintaku and Juan G. Santiago, date: 06/20/2016, view: 1988, Q&A: 0
This protocol describes the extraction, fractionation, and recovery of cytoplasmic nucleic acids (e.g., cytoplasmic RNA) versus nucleic acids in the cell nucleus (including genomic DNA, gDNA) from single cells with a microfluidic system. ...