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Kayleigh Dodd Institute of Cancer and Genetics, Cardiff University, UK
1 protocol

Andrew Robert Tee
  • Institute of Cancer and Genetics, Cardiff University, UK
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  • 1 Author merit
1 Protocol published
In vitro mTORC1 Kinase Assay for Mammalian Cells Protocol
Authors:  Kayleigh Margaret Dodd and Andrew Robert Tee, date: 06/05/2016, view: 2269, Q&A: 0
Historically, mechanistic target of rapamycin (mTOR) was purified from mammalian cells using mild nonionic detergents such as NP-40 and or Triton-X100 that resulted in dissociation of core regulatory components essential for its native kinase ...