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Coauthors
Amy Kiger Division of Biological Sciences, University of California, USA
2 protocols
Coreviewers
Chunjing Qu Baylor Genetics Laboratories/Baylor College of Medicine, USA
2 protocols

HongLok Lung Department of Clinical Oncology and Center for Cancer Research, The University of Hong Kong, Hong Kong SAR
56 protocols

Jihyun Kim Bio-Protocol
5 protocols

Steve Jean
  • Département d'anatomie et de biologie cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Canada
Research focus
  • Cell biology
Contributions
  • 2 Author merit
  • 2 Reviewer merit

Education

Ph.D. in Molecular and Cell Biology, Laval University, Québec, Canada, 2008

Current position

Assistant Professor, Department of Anatomy and Cell Biology, Université de Sherbrooke, Sherbrooke, Canada

Publications

  1. Jean, S., Cox, S., Nassari, S. and Kiger, A. A. (2015). Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion. EMBO Rep 16(3): 297-311.
  2. Jean, S. and Kiger, A. A. (2014). Classes of phosphoinositide 3-kinases at a glance. J Cell Sci 127(Pt 5): 923-928.
  3. Jean, S. and Kiger, A. A. (2012). Coordination between RAB GTPase and phosphoinositide regulation and functions. Nat Rev Mol Cell Biol 13(7): 463-470.
  4. Jean, S., Cox, S., Schmidt, E. J., Robinson, F. L. and Kiger, A. (2012). Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. Mol Biol Cell 23(14): 2723-2740.
  5. Jean, S., Tremblay, M. G., Herdman, C., Guillou, F. and Moss, T. (2012). The endocytic adapter E-Syt2 recruits the p21 GTPase activated kinase PAK1 to mediate actin dynamics and FGF signalling. Biol Open 1(8): 731-738.
  6. Jean, S., Mikryukov, A., Tremblay, M. G., Baril, J., Guillou, F., Bellenfant, S. and Moss, T. (2010). Extended-synaptotagmin-2 mediates FGF receptor endocytosis and ERK activation in vivo. Dev Cell 19(3): 426-439.
  7. Velichkova, M., Juan, J., Kadandale, P., Jean, S., Ribeiro, I., Raman, V., Stefan, C. and Kiger, A. A. (2010). Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions. J Cell Biol 190(3): 407-425.
  8. Jean, S. and Moss, T. (2008). A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus. Differentiation 76(4): 431-441.
  9. Bisson, N., Islam, N., Poitras, L., Jean, S., Bresnick, A. and Moss, T. (2003). The catalytic domain of xPAK1 is sufficient to induce myosin II dependent in vivo cell fragmentation independently of other apoptotic events. Dev Biol 263(2): 264-281.
  10. Poitras, L., Jean, S., Islam, N. and Moss, T. (2003). PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase. FEBS Lett 543(1-3): 129-135.
2 Protocols published
VAMP8-3xHA Uptake Assay in HeLa Cells
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 2056, Q&A: 0
Transmembrane proteins are rarely exclusively localized to a specific vesicle or an organelle. Most transmembrane proteins undergo complicated trafficking routes. Thus, transmembrane proteins are under constant flux, and at steady state, found on a ...
RAB21 Activity Assay Using GST-fused APPL1
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 2022, Q&A: 0
The Rab family of small GTPases are essential regulators of membrane trafficking events. As with other small GTPase families, Rab GTPases cycle between an inactive GDP- bound state and an active GTP-bound state. Guanine nucleotide exchange factors ...
2 Protocols reviewed
Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells
Authors:  Benjamin E. Housden, Hilary E. Nicholson and Norbert Perrimon, date: 02/05/2017, view: 1766, Q&A: 0
A synthetic lethal interaction is a type of genetic interaction where the disruption of either of two genes individually has little effect but their combined disruption is lethal. Knowledge of synthetic lethal interactions can allow for elucidation ...
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Lentiviral shRNA Screen to Identify Epithelial Integrity Regulating Genes in MCF10A 3D Culture
Authors:  Elsa Marques and Juha Klefström, date: 12/05/2016, view: 1854, Q&A: 0
MCF10A 3D culture system provides a reductionist model of glandular mammary epithelium which is widely used to study development of glandular architecture, the role of cell polarity and epithelial integrity in control of epithelial cell functions, ...
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