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Coauthors
Scott Lowe Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, USA
2 protocols

Sarah Ackerman Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, USA
1 protocol

Kevin O’Rourke Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, USA
2 protocols

Lukas E Dow
  • Department of Medicine, Weill Cornell Medical College, USA
Contributions
  • 2 Author merit
2 Protocols published
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Authors:  Kevin P. O’Rourke, Sarah Ackerman, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 5914, Q&A: 3
In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and ...
Immunofluorescent Staining of Mouse Intestinal Stem Cells
Authors:  Kevin P. O’Rourke, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 6263, Q&A: 0
Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including ...