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Coauthors
Wolf Frommer Department of Plant Biology, Carnegie Science, USA
2 protocols
Coreviewers
Neelanjan Bose University of California, San Francisco
16 protocols

Ria Sircar Stanford University
2 protocols

Reviewer
Cheng-Hsun Ho
  • Assistant professor, Agricultural Biotechnology Research Center of Academia Sinica
Research focus
  • Plant science
  • Nitrogen, transporter, and FRET biosensor
Contributions
  • 1 Author merit
  • 2 Reviewer merit

Education

PhD

Lab information

Cheng-Hsun Ho's Lab

Publications

Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho & Wolf B. Frommer(2017) Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.Nature Communications 8, 431.
Ho C.H. & Frommer W.B. (2016) Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Activity Sensors in Yeast Cultures. Bio-protocol, vol 6, iss 3
Ho C.H. & Frommer W.B. (2014) Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporter. eLife 3, e01917.
Jones A., Lalonde S., Ho C.H., Xu M., Wang R.S., Xuan Y., You C.H., Sardi M.I., Parsa S.A., Smith-Valle E., Su T.Y., Frazer K., Pilot G., Pratelli R., Grossmann G., Acharya B.R., Hu H.C., Engineer C., Villiers F., Takeda K., Zhao SU., Dong Q.F., Assmann S.M, Chen J., Kwak J.M., Schroeder J.I., Albert R., Rhee S.Y., & Frommer W.B. (2014) Border control – a membrane-linked interactome of Arabidopsis. Science. 344(6185):711-6
De Michele R., Ast C., Loqué D., Ho C.H., Andrade S.L.A., Grossmann G., Gehne S., Kumke M.U. & Frommer W.B. (2013) Fluorescent sensors reporting the activity of ammonium transceptors in live cells. eLife 2, e00800.
Jones A., Grossmann G., Danielson, J.A.H., Sosso D., Chen L.Q., Ho C.H. & Frommer W.B. (2013) In vivo biochemistry: Applications for small molecule biosensors in plant biology. Curr. Opin. Plant Biol. 16: 389-95.
Tsay Y.F., Ho C.H., Chen H.Y. & Lin S.H. (2011) Integration of Nitrogen and Potassium Signaling. Annu Rev Plant Biol. 62:207-26.
Ho C.H. & Tsay Y.F. (2010) Nitrate, ammonium, and potassium sensing and signaling. Curr Opin Plant Biol. 13(5): 604-10.
Ho C.H., Lin S.H., Hu H.C. & Tsay Y.F. (2009) CHL1 functions as a nitrate sensor in plants. Cell. 138(6): 1184-94.
Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H. & Hsu P.K. (2007) Nitrate transporters and peptide transporters. FEBS Lett. 581(12): 2290-300.

1 Protocol published
Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Activity Sensors in Yeast Cultures
Authors:  Cheng-Hsun Ho and Wolf B. Frommer, date: 02/05/2016, view: 2757, Q&A: 0
This protocol describes the methods used to engineer and deploy genetically encoded fluorescence activity reporters for nitrate and peptide transporter activity in yeast cells. Fusion of the dual-affinity nitrate transceptor CHL1/AtNRT1.1/AtNPF6.3 ...
2 Protocols reviewed
Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes
Authors:  Shujie Shi and Marcelo D. Carattino, date: 04/20/2017, view: 1282, Q&A: 0
Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by ...
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