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Coauthors
Angela Corona University of Cagliari
1 protocol

Enzo Tramontano
  • Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Italy
Contributions
  • 1 Author merit
1 Protocol published
RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes
Authors:  Angela Corona and Enzo Tramontano, date: 08/20/2015, view: 2855, Q&A: 0
The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA ...