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Coauthors
Andreas Bachmair Dept. of Biochemistry and Cell Biology, Max F. Perutz Laboratories, Vienna BioCenter, University of Vienna, Austria
1 protocol

Konstantin Tomanov
  • Dept. of Biochemistry and Cell Biology, Max F. Perutz Laboratories, Vienna BioCenter, University of Vienna, Austria
Contributions
  • 1 Author merit
1 Protocol published
Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
Authors:  Konstantin Tomanov and Andreas Bachmair, date: 08/05/2015, view: 1843, Q&A: 0
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E. coli) ...