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Coauthors
David Traynor MRC Laboratory of Molecular Biology, UK
1 protocol

Robert Kay MRC Laboratory of Molecular Biology, Cambridge
1 protocol

Reviewer
Jennifer Hirst
  • University of Cambridge
Contributions
  • 1 Author merit

Education

Ph.D. in Cell Biology, University College London, UK, 1994

Current position

Principal Research Associate, Department of Clinical Biochemistry, CIMR, Wellcome Trust/MRC Building, Addenbrooke’s Hospital, Cambridge, UK

Publications (since 2010)

  1. Hardies, K., May, P., Djemie, T., Tarta-Arsene, O., Deconinck, T., Craiu, D., Consortium, A. R. w. g. o. t. E. R., Helbig, I., Suls, A., Balling, R., Weckhuysen, S., De Jonghe, P. and Hirst, J. (2015). Recessive loss-of-function mutations in AP4S1 cause mild fever-sensitive seizures, developmental delay and spastic paraplegia through loss of AP-4 complex assembly. Hum Mol Genet 24(8): 2218-2227.
  2. Zhang, Y., Persson, S., Hirst, J., Robinson, M. S., van Damme, D. and Sanchez-Rodriguez, C. (2015). Change your TPLATE, change your fate: plant CME and beyond. Trends Plant Sci 20(1): 41-48.
  3. Borner, G. H., Hein, M. Y., Hirst, J., Edgar, J. R., Mann, M. and Robinson, M. S. (2014). Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions. Mol Biol Cell 25(20): 3178-3194.
  4. Hirst, J., Schlacht, A., Norcott, J. P., Traynor, D., Bloomfield, G., Antrobus, R., Kay, R. R., Dacks, J. B. and Robinson, M. S. (2014). Characterization of TSET, an ancient and widespread membrane trafficking complex. Elife 3: e02866.
  5. Robinson, M. S. and Hirst, J. (2013). Rapid inactivation of proteins by knocksideways. Curr Protoc Cell Biol 61: 15 20 11-17.
  6. Hirst, J., Borner, G. H., Edgar, J., Hein, M. Y., Mann, M., Buchholz, F., Antrobus, R. and Robinson, M. S. (2013). Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15. Mol Biol Cell 24(16): 2558-2569.
  7. Hirst, J., Irving, C. and Borner, G. H. (2013). Adaptor protein complexes AP-4 and AP-5: new players in endosomal trafficking and progressive spastic paraplegia. Traffic 14(2): 153-164.
  8. Kong, X. F., Bousfiha, A., Rouissi, A., Itan, Y., Abhyankar, A., Bryant, V., Okada, S., Ailal, F., Bustamante, J., Casanova, J. L., Hirst, J. and Boisson-Dupuis, S. (2013). A novel homozygous p.R1105X mutation of the AP4E1 gene in twins with hereditary spastic paraplegia and mycobacterial disease. PLoS One 8(3): e58286.
  9. Pohler, E., Mamai, O., Hirst, J., Zamiri, M., Horn, H., Nomura, T., Irvine, A. D., Moran, B., Wilson, N. J., Smith, F. J., Goh, C. S., Sandilands, A., Cole, C., Barton, G. J., Evans, A. T., Shimizu, H., Akiyama, M., Suehiro, M., Konohana, I., Shboul, M., Teissier, S., Boussofara, L., Denguezli, M., Saad, A., Gribaa, M., Dopping-Hepenstal, P. J., McGrath, J. A., Brown, S. J., Goudie, D. R., Reversade, B., Munro, C. S. and McLean, W. H. (2012). Haploinsufficiency for AAGAB causes clinically heterogeneous forms of punctate palmoplantar keratoderma. Nat Genet 44(11): 1272-1276.
  10. Hirst, J., Borner, G. H., Antrobus, R., Peden, A. A., Hodson, N. A., Sahlender, D. A. and Robinson, M. S. (2012). Distinct and overlapping roles for AP-1 and GGAs revealed by the "knocksideways" system. Curr Biol 22(18): 1711-1716.
  11. Borner, G. H., Antrobus, R., Hirst, J., Bhumbra, G. S., Kozik, P., Jackson, L. P., Sahlender, D. A. and Robinson, M. S. (2012). Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles. J Cell Biol 197(1): 141-160.
  12. Hirst, J., Barlow, L. D., Francisco, G. C., Sahlender, D. A., Seaman, M. N., Dacks, J. B. and Robinson, M. S. (2011). The fifth adaptor protein complex. PLoS Biol 9(10): e1001170.
  13. Hirst, J. and Carmichael, J. (2011). A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis. BMC Cell Biol 12: 22.
1 Protocol published
Dictyostelium Cultivation, Transfection, Microscopy and Fractionation
Authors:  Jennifer Hirst, Robert R Kay and David Traynor, date: 06/05/2015, view: 4688, Q&A: 0
The real time visualisation of fluorescently tagged proteins in live cells using ever more sophisticated microscopes has greatly increased our understanding of the dynamics of key proteins during fundamental physiological processes such as cell ...