Anja Schmidt Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Switzerland
1 protocol

Christian Sailer Swiss Federal Institute for Forrest, Snow and Landscape Research WSL
1 protocol

Ueli Grossniklaus
  • Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Switzerland
  • 1 Author merit


Ph.D. (Dr, phil. II) in Developmental Biology and Genetics, Faculty of Science, Biozentrum, University of Basel, 1993
M.Sc. (Diplom) in Biology II (major: Cell Biology), Faculty of Science, Biozentrum, University of Basel, 1989

Current position

Professor of Plant Developmental Genetics, Institute of Plant Biology, University of Zürich, Switzerland

Publications (since 2010)

  1. Kessler, S. A., Lindner, H., Jones, D. S. and Grossniklaus, U. (2015). Functional analysis of related CrRLK1L receptor-like kinases in pollen tube reception. EMBO Rep 16(1): 107-115.
  2. Schmid, M. W. and Grossniklaus, U. (2015). Rcount: simple and flexible RNA-Seq read counting. Bioinformatics 31(3): 436-437.
  3. Schmidt, A., Schmid, M. W. and Grossniklaus, U. (2015). Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction. Development 142(2): 229-241.
  4. Vogler, H., Felekis, D., Nelson, B. J. and Grossniklaus, U. (2015). Measuring the Mechanical Properties of Plant Cell Walls. Plants 4(2): 167-182.
  5. Singh, A. K., Bashir, T., Sailer, C., Gurumoorthy, V., Ramakrishnan, A. M., Dhanapal, S., Grossniklaus, U. and Baskar, R. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol 168(1): 247-257.
  6. Felekis, D., Vogler, H., Mecja, G., Muntwyler, S., Nestorova, A., Huang, T., Sakar, M. S., Grossniklaus, U. and Nelson, B. J. (2015). Real-time automated characterization of 3D morphology and mechanics of developing plant cells. The International Journal of Robotics Research: 0278364914564231.
  7. Felekis D, Vogler H, Grossniklaus U, Nelson BJ (2015). Microrobotic tools for plant biology. In Emerging Tools for Micro and Nano Manipulation, Wiley-VCH, in press.
  8. Lindner, H., Kessler, S. A., Muller, L. M., Shimosato-Asano, H., Boisson-Dernier, A. and Grossniklaus, U. (2015). TURAN and EVAN mediate pollen tube reception in Arabidopsis Synergids through protein glycosylation. PLoS Biol 13(4): e1002139.
  9. Gossmann, T. I., Schmid, M. W., Grossniklaus, U. and Schmid, K. J. (2014). Selection-driven evolution of sex-biased genes is consistent with sexual selection in Arabidopsis thaliana. Mol Biol Evol 31(3): 574-583.
  10. Sailer, C., Schmid, B., Stöcklin, J. and Grossniklaus, U. (2014). Sexual Hieracium pilosella plants are better inter-specific, while apomictic plants are better intra-specific competitors. Perspectives in Plant Ecology, Evolution and Systematics 16(2): 43-51.
  11. Bashir, T., Sailer, C., Gerber, F., Loganathan, N., Bhoopalan, H., Eichenberger, C., Grossniklaus, U. and Baskar, R. (2014). Hybridization alters spontaneous mutation rates in a parent-of-origin-dependent fashion in Arabidopsis. Plant Physiol 165(1): 424-437.
  12. Ngo, Q. A., Vogler, H., Lituiev, D. S., Nestorova, A. and Grossniklaus, U. (2014). A calcium dialog mediated by the FERONIA signal transduction pathway controls plant sperm delivery. Dev Cell 29(4): 491-500.
  13. Schmidt, A., Schmid, M. W., Klostermeier, U. C., Qi, W., Guthorl, D., Sailer, C., Waller, M., Rosenstiel, P. and Grossniklaus, U. (2014). Apomictic and sexual germline development differ with respect to cell cycle, transcriptional, hormonal and epigenetic regulation. PLoS Genet 10(7): e1004476.
  14. Grob, S., Schmid, M. W. and Grossniklaus, U. (2014). Hi-C analysis in Arabidopsis identifies the KNOT, a structure with similarities to the flamenco locus of Drosophila. Mol Cell 55(5): 678-693.
  15. Felekis, D., Vogler, H., Mecja, G., Muntwyler, S., Sakar, M. S., Grossniklaus, U. and Nelson, B. J. (2014). High-throughput analysis of the morphology and mechanics of tip growing cells using a microrobotic platform. Intelligent Robots and Systems (IROS 2014), 2014 IEEE/RSJ International Conference on, IEEE.
  16. Lituiev, D. S. and Grossniklaus, U. (2014). Patterning of the angiosperm female gametophyte through the prism of theoretical paradigms. Biochem Soc Trans 42(2): 332-339.
  17. Pires, N. D. and Grossniklaus, U. (2014). Different yet similar: evolution of imprinting in flowering plants and mammals. F1000Prime Rep 6: 63.
  18. Grossniklaus, U. and Paro, R. (2014). Transcriptional silencing by polycomb-group proteins. Cold Spring Harb Perspect Biol 6(11): a019331.
  19. Wuest, S. E. and Grossniklaus, U. (2014). Laser-assisted microdissection applied to floral tissues. Methods Mol Biol 1110: 329-344.
  20. Ngo, Q. A., Albrecht, H., Tsuchimatsu, T. and Grossniklaus, U. (2013). The differentially regulated genes TvQR1 and TvPirin of the parasitic plant Triphysaria exhibit distinctive natural allelic diversity. BMC Plant Biol 13: 28.
  21. Vogler, H., Draeger, C., Weber, A., Felekis, D., Eichenberger, C., Routier-Kierzkowska, A. L., Boisson-Dernier, A., Ringli, C., Nelson, B. J., Smith, R. S. and Grossniklaus, U. (2013). The pollen tube: a soft shell with a hard core. Plant J 73(4): 617-627.
  22. Schmidt, A., Wohrmann, H. J., Raissig, M. T., Arand, J., Gheyselinck, J., Gagliardini, V., Heichinger, C., Walter, J. and Grossniklaus, U. (2013). The Polycomb group protein MEDEA and the DNA methyltransferase MET1 interact to repress autonomous endosperm development in Arabidopsis. Plant J 73(5): 776-787.
  23. Lindner, M., Simonini, S., Kooiker, M., Gagliardini, V., Somssich, M., Hohenstatt, M., Simon, R., Grossniklaus, U. and Kater, M. M. (2013). TAF13 interacts with PRC2 members and is essential for Arabidopsis seed development. Dev Biol 379(1): 28-37.
  24. Sedeek, K. E., Qi, W., Schauer, M. A., Gupta, A. K., Poveda, L., Xu, S., Liu, Z. J., Grossniklaus, U., Schiestl, F. P. and Schluter, P. M. (2013). Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids. PLoS One 8(5): e64621.
  25. Raissig, M. T., Gagliardini, V., Jaenisch, J., Grossniklaus, U. and Baroux, C. (2013). Efficient and rapid isolation of early-stage embryos from Arabidopsis thaliana seeds. J Vis Exp(76).
  26. Lituiev, D. S., Krohn, N. G., Muller, B., Jackson, D., Hellriegel, B., Dresselhaus, T. and Grossniklaus, U. (2013). Theoretical and experimental evidence indicates that there is no detectable auxin gradient in the angiosperm female gametophyte. Development 140(22): 4544-4553.
  27. Grob, S., Schmid, M. W., Luedtke, N. W., Wicker, T. and Grossniklaus, U. (2013). Characterization of chromosomal architecture in Arabidopsis by chromosome conformation capture. Genome Biol 14(11): R129.
  28. Boisson-Dernier, A., Lituiev, D. S., Nestorova, A., Franck, C. M., Thirugnanarajah, S. and Grossniklaus, U. (2013). ANXUR receptor-like kinases coordinate cell wall integrity with growth at the pollen tube tip via NADPH oxidases. PLoS Biol 11(11): e1001719.
  29. Raissig, M. T., Bemer, M., Baroux, C. and Grossniklaus, U. (2013). Genomic imprinting in the Arabidopsis embryo is partly regulated by PRC2. PLoS Genet 9(12): e1003862.
  30. Wuest, S. E., Schmid, M. W. and Grossniklaus, U. (2013). Cell-specific expression profiling of rare cell types as exemplified by its impact on our understanding of female gametophyte development. Curr Opin Plant Biol 16(1): 41-49.
  31. Baroux, C., Autran, D., Raissig, M. T., Grimanelli, D. and Grossniklaus, U. (2013). Parental contributions to the transcriptome of early plant embryos. Curr Opin Genet Dev 23(1): 72-74.
  32. Grossniklaus, U., Kelly, W. G., Ferguson-Smith, A. C., Pembrey, M. and Lindquist, S. (2013). Transgenerational epigenetic inheritance: how important is it? Nat Rev Genet 14(3): 228-235.
  33. Barrell, P. and Grossniklaus, U. (2013). Examining female meiocytes of maize by confocal microscopy. Methods Mol Biol 990: 45-52.
  34. Hirsch S, Baumberger R, Grossniklaus U (2013). Epigenetic variation, inheritance, and selection in plant populations. Cold Spring Harb Symp Quant Biol 77, 97-104.
  35. Schmid, M. W., Schmidt, A., Klostermeier, U. C., Barann, M., Rosenstiel, P. and Grossniklaus, U. (2012). A powerful method for transcriptional profiling of specific cell types in eukaryotes: laser-assisted microdissection and RNA sequencing. PLoS One 7(1): e29685.
  36. Petricka, J. J., Schauer, M. A., Megraw, M., Breakfield, N. W., Thompson, J. W., Georgiev, S., Soderblom, E. J., Ohler, U., Moseley, M. A., Grossniklaus, U. and Benfey, P. N. (2012). The protein expression landscape of the Arabidopsis root. Proc Natl Acad Sci U S A 109(18): 6811-6818.
  37. You, W., Tyczewska, A., Spencer, M., Daxinger, L., Schmid, M. W., Grossniklaus, U., Simon, S. A., Meyers, B. C., Matzke, A. J. and Matzke, M. (2012). Atypical DNA methylation of genes encoding cysteine-rich peptides in Arabidopsis thaliana. BMC Plant Biol 12: 51.
  38. Mayank, P., Grossman, J., Wuest, S., Boisson-Dernier, A., Roschitzki, B., Nanni, P., Nuhse, T. and Grossniklaus, U. (2012). Characterization of the phosphoproteome of mature Arabidopsis pollen. Plant J 72(1): 89-101.
  39. Lindner, H., Raissig, M. T., Sailer, C., Shimosato-Asano, H., Bruggmann, R. and Grossniklaus, U. (2012). SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing. Genetics 191(4): 1381-1386.
  40. Marshall, A., Aalen, R. B., Audenaert, D., Beeckman, T., Broadley, M. R., Butenko, M. A., Cano-Delgado, A. I., de Vries, S., Dresselhaus, T., Felix, G., Graham, N. S., Foulkes, J., Granier, C., Greb, T., Grossniklaus, U., Hammond, J. P., Heidstra, R., Hodgman, C., Hothorn, M., Inze, D., Ostergaard, L., Russinova, E., Simon, R., Skirycz, A., Stahl, Y., Zipfel, C. and De Smet, I. (2012). Tackling drought stress: receptor-like kinases present new approaches. Plant Cell 24(6): 2262-2278.
  41. Wohrmann, H. J., Gagliardini, V., Raissig, M. T., Wehrle, W., Arand, J., Schmidt, A., Tierling, S., Page, D. R., Schob, H., Walter, J. and Grossniklaus, U. (2012). Identification of a DNA methylation-independent imprinting control region at the Arabidopsis MEDEA locus. Genes Dev 26(16): 1837-1850.
  42. Leshem, Y., Johnson, C., Wuest, S. E., Song, X., Ngo, Q. A., Grossniklaus, U. and Sundaresan, V. (2012). Molecular characterization of the glauce mutant: a central cell-specific function is required for double fertilization in Arabidopsis. Plant Cell 24(8): 3264-3277.
  43. Xu, S., Schluter, P. M., Grossniklaus, U. and Schiestl, F. P. (2012). The genetic basis of pollinator adaptation in a sexually deceptive orchid. PLoS Genet 8(8): e1002889.
  44. Kritsas, K., Wuest, S. E., Hupalo, D., Kern, A. D., Wicker, T. and Grossniklaus, U. (2012). Computational analysis and characterization of UCE-like elements (ULEs) in plant genomes. Genome Res 22(12): 2455-2466.
  45. Zust, T., Heichinger, C., Grossniklaus, U., Harrington, R., Kliebenstein, D. J. and Turnbull, L. A. (2012). Natural enemies drive geographic variation in plant defenses. Science 338(6103): 116-119.
  46. Ngo, Q. A., Baroux, C., Guthorl, D., Mozerov, P., Collinge, M. A., Sundaresan, V. and Grossniklaus, U. (2012). The Armadillo repeat gene ZAK IXIK promotes Arabidopsis early embryo and endosperm development through a distinctive gametophytic maternal effect. Plant Cell 24(10): 4026-4043.
  47. Iwano, M., Ngo, Q. A., Entani, T., Shiba, H., Nagai, T., Miyawaki, A., Isogai, A., Grossniklaus, U. and Takayama, S. (2012). Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen tube with synergid cells. Development 139(22): 4202-4209.
  48. Sprunck, S., Rademacher, S., Vogler, F., Gheyselinck, J., Grossniklaus, U. and Dresselhaus, T. (2012). Egg cell-secreted EC1 triggers sperm cell activation during double fertilization. Science 338(6110): 1093-1097.
  49. Jones, A. M., Aebersold, R., Ahrens, C. H., Apweiler, R., Baerenfaller, K., Baker, M., Bendixen, E., Briggs, S., Brownridge, P., Brunner, E., Daube, M., Deutsch, E. W., Grossniklaus, U., Heazlewood, J., Hengartner, M. O., Hermjakob, H., Jovanovic, M., Lawless, C., Lochnit, G., Martens, L., Ravnsborg, C., Schrimpf, S. P., Shim, Y. H., Subasic, D., Tholey, A., Wijk, K., Mering, C., Weiss, M. and Zheng, X. (2012). The HUPO initiative on Model Organism Proteomes, iMOP. Proteomics 12(3): 340-345.
  50. Schmidt, A., Schmid, M. W. and Grossniklaus, U. (2012). Analysis of plant germline development by high-throughput RNA profiling: technical advances and new insights. Plant J 70(1): 18-29.
  51. Lindner, H., Muller, L. M., Boisson-Dernier, A. and Grossniklaus, U. (2012). CrRLK1L receptor-like kinases: not just another brick in the wall. Curr Opin Plant Biol 15(6): 659-669.
  52. Pires, N. D. and Grossniklaus, U. (2012). How to fine-tune an epigenetic switch. Dev Cell 23(3): 453-454.
  53. Bemer, M. and Grossniklaus, U. (2012). Dynamic regulation of Polycomb group activity during plant development. Curr Opin Plant Biol 15(5): 523-529.
  54. Hirsch, S., Baumberger, R. and Grossniklaus, U. (2012). Epigenetic variation, inheritance, and selection in plant populations. Cold Spring Harb Symp Quant Biol 77: 97-104.
  55. Dumbliauskas, E., Lechner, E., Jaciubek, M., Berr, A., Pazhouhandeh, M., Alioua, M., Cognat, V., Brukhin, V., Koncz, C., Grossniklaus, U., Molinier, J. and Genschik, P. (2011). The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting. EMBO J 30(4): 731-743.
  56. Brukhin, V. B., Jaciubek, M., Bolanos Carpio, A., Kuzmina, V. and Grossniklaus, U. (2011). Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen. Int J Dev Biol 55(1): 73-84.
  57. Felekis, D., Muntwyler, S., Vogler, H., Beyeler, F., Grossniklaus, U. and Nelson, B. (2011). Quantifying growth mechanics of living, growing plant cells in situ using microrobotics. Micro & Nano Letters 6(5): 311-316.
  58. Koszegi, D., Johnston, A. J., Rutten, T., Czihal, A., Altschmied, L., Kumlehn, J., Wust, S. E., Kirioukhova, O., Gheyselinck, J., Grossniklaus, U. and Baumlein, H. (2011). Members of the RKD transcription factor family induce an egg cell-like gene expression program. Plant J 67(2): 280-291.
  59. Schluter, P. M., Xu, S., Gagliardini, V., Whittle, E., Shanklin, J., Grossniklaus, U. and Schiestl, F. P. (2011). Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids. Proc Natl Acad Sci U S A 108(14): 5696-5701.
  60. Autran, D., Baroux, C., Raissig, M. T., Lenormand, T., Wittig, M., Grob, S., Steimer, A., Barann, M., Klostermeier, U. C., Leblanc, O., Vielle-Calzada, J. P., Rosenstiel, P., Grimanelli, D. and Grossniklaus, U. (2011). Maternal epigenetic pathways control parental contributions to Arabidopsis early embryogenesis. Cell 145(5): 707-719.
  61. Kirioukhova, O., Johnston, A. J., Kleen, D., Kagi, C., Baskar, R., Moore, J. M., Baumlein, H., Gross-Hardt, R. and Grossniklaus, U. (2011). Female gametophytic cell specification and seed development require the function of the putative Arabidopsis INCENP ortholog WYRD. Development 138(16): 3409-3420.
  62. McKeown, P. C., Laouielle-Duprat, S., Prins, P., Wolff, P., Schmid, M. W., Donoghue, M. T., Fort, A., Duszynska, D., Comte, A., Lao, N. T., Wennblom, T. J., Smant, G., Kohler, C., Grossniklaus, U. and Spillane, C. (2011). Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds. BMC Plant Biol 11: 113.
  63. Schmidt, A., Wuest, S. E., Vijverberg, K., Baroux, C., Kleen, D. and Grossniklaus, U. (2011). Transcriptome analysis of the Arabidopsis megaspore mother cell uncovers the importance of RNA helicases for plant germline development. PLoS Biol 9(9): e1001155.
  64. Boisson-Dernier, A., Kessler, S. A. and Grossniklaus, U. (2011). The walls have ears: the role of plant CrRLK1Ls in sensing and transducing extracellular signals. J Exp Bot 62(5): 1581-1591.
  65. Raissig, M. T., Baroux, C. and Grossniklaus, U. (2011). Regulation and flexibility of genomic imprinting during seed development. Plant Cell 23(1): 16-26.
  66. Baroux, C., Raissig, M. T. and Grossniklaus, U. (2011). Epigenetic regulation and reprogramming during gamete formation in plants. Curr Opin Genet Dev 21(2): 124-133.
  67. Paszkowski, J. and Grossniklaus, U. (2011). Selected aspects of transgenerational epigenetic inheritance and resetting in plants. Curr Opin Plant Biol 14(2): 195-203.
  68. Grossniklaus, U. (2011). Plant germline development: a tale of cross-talk, signaling, and cellular interactions. Sex Plant Reprod 24(2): 91-95.
  69. Kessler, S. A. and Grossniklaus, U. (2011). She's the boss: signaling in pollen tube reception. Curr Opin Plant Biol 14(5): 622-627.
  70. Aquea, F., Johnston, A. J., Canon, P., Grossniklaus, U. and Arce-Johnson, P. (2010). TRAUCO, a Trithorax-group gene homologue, is required for early embryogenesis in Arabidopsis thaliana. J Exp Bot 61(4): 1215-1224.
  71. Pillot, M., Baroux, C., Vazquez, M. A., Autran, D., Leblanc, O., Vielle-Calzada, J. P., Grossniklaus, U. and Grimanelli, D. (2010). Embryo and endosperm inherit distinct chromatin and transcriptional states from the female gametes in Arabidopsis. Plant Cell 22(2): 307-320.
  72. Wuest, S. E., Vijverberg, K., Schmidt, A., Weiss, M., Gheyselinck, J., Lohr, M., Wellmer, F., Rahnenfuhrer, J., von Mering, C. and Grossniklaus, U. (2010). Arabidopsis female gametophyte gene expression map reveals similarities between plant and animal gametes. Curr Biol 20(6): 506-512.
  73. Matias-Hernandez, L., Battaglia, R., Galbiati, F., Rubes, M., Eichenberger, C., Grossniklaus, U., Kater, M. M. and Colombo, L. (2010). VERDANDI is a direct target of the MADS domain ovule identity complex and affects embryo sac differentiation in Arabidopsis. Plant Cell 22(6): 1702-1715.
  74. Johnston, A. J., Kirioukhova, O., Barrell, P. J., Rutten, T., Moore, J. M., Baskar, R., Grossniklaus, U. and Gruissem, W. (2010). Dosage-sensitive function of retinoblastoma related and convergent epigenetic control are required during the Arabidopsis life cycle. PLoS Genet 6(6): e1000988.
  75. Fakheran, S., Paul-Victor, C., Heichinger, C., Schmid, B., Grossniklaus, U. and Turnbull, L. A. (2010). Adaptation and extinction in experimentally fragmented landscapes. Proc Natl Acad Sci U S A 107(44): 19120-19125.
  76. Keinath, N. F., Kierszniowska, S., Lorek, J., Bourdais, G., Kessler, S. A., Shimosato-Asano, H., Grossniklaus, U., Schulze, W. X., Robatzek, S. and Panstruga, R. (2010). PAMP (pathogen-associated molecular pattern)-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity. J Biol Chem 285(50): 39140-39149.
  77. Kessler, S. A., Shimosato-Asano, H., Keinath, N. F., Wuest, S. E., Ingram, G., Panstruga, R. and Grossniklaus, U. (2010). Conserved molecular components for pollen tube reception and fungal invasion. Science 330(6006): 968-971.
  78. Muller, B. and Grossniklaus, U. (2010). Model organisms--A historical perspective. J Proteomics 73(11): 2054-2063.
  79. Pien, S. and Grossniklaus, U. (2010). Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis. Methods Mol Biol 631: 209-220.
1 Protocol published
Determination of the Developmental Origin of Seeds Containing Endosperm Using Flow Cytometric Analysis
Authors:  Christian Sailer, Anja Schmidt and Ueli Grossniklaus, date: 06/05/2015, view: 2459, Q&A: 0
Seeds derived from a diploid, sexual plant typically contain a 2n embryo (n+n) and 3n endosperm, a ratio characteristic for most flowering plants. However, this ratio is altered in apomictic species, which reproduce asexually through seeds (Koltunow ...