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Coauthors
Yoshikazu Kiriiwa Agriculture Department, Shizuoka University, Japan
1 protocol

Chikako Fukazawa Agriculture Department, Shizuoka University, Japan
1 protocol

Harumi Enoki Agriculture Department, Shizuoka University, Japan
1 protocol

Fumiyoshi Myouga RIKEN Center for Sustainable Resource Science, Japan
2 protocols

Reiko Motohashi
  • Agriculture Department, Shizuoka University, Japan
Contributions
  • 3 Author merit

Education

Ph.D. in Agriculture, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1997

Current position

Professor in the Agriculture Department, Shizuoka University, Shizuoka, Japan

Publication

  1. Asai, T., Choi, J. H, Ikka, T., Fushimi, K., Abe, N., Tanaka, H., Yamakawa, Y., Kobori, H., Kiriiwa, Y., Motohashi, R., Deo, V. P., Asakawa, T., Kan, T., Morita, A., Kawagishi, H. (2015). Effect of 2-azahypoxanthine (AHX) produced by the fairy-ring-forming fungus on the growth and the grain yield of rice. JARQ 49(1),45-49.
  2. Satou, M., Enoki, H., Oikawa, A., Ohta, D., Saito, K., Hachiya, T., Sakakibara, H., Kusano, M., Fukushima, A., Saito, K., Kobayashi, M., Nagata, N., Myouga, F., Shinozaki, K. and Motohashi, R. (2014). Integrated analysis of transcriptome and metabolome of Arabidopsis albino or pale green mutants with disrupted nuclear-encoded chloroplast proteins. Plant Mol Biol 85(4-5): 411-428.
  3. Choi, J. H., Ohnishi, T., Yamakawa, Y., Takeda, S., Sekiguchi, S., Maruyama, W., Yamashita, K., Suzuki, T., Morita, A., Ikka, T., Motohashi, R., Kiriiwa, Y., Tobina, H., Asai, T., Tokuyama, S., Hirai, H., Yasuda, N., Noguchi, K., Asakawa, T., Sugiyama, S., Kan, T. and Kawagishi, H. (2014). The source of "fairy rings": 2-azahypoxanthine and its metabolite found in a novel purine metabolic pathway in plants. Angew Chem Int Ed Engl 53(6): 1552-1555.
  4. Ma, G., Zhang, L., Matsuta, A., Matsutani, K., Yamawaki, K., Yahata, M., Wahyudi, A., Motohashi, R. and Kato, M. (2013). Enzymatic formation of beta-citraurin from beta-cryptoxanthin and Zeaxanthin by carotenoid cleavage dioxygenase4 in the flavedo of citrus fruit. Plant Physiol 163(2): 682-695.
  5. Homma, M. K.#, Motohashi, R.# and Ohtsubo, H. (2013). Maximizing the potential of scientists in Japan: promoting equal participation for women scientists through leadership development. Genes Cells 18(7): 529-532.(#Equal contribution)
  6. Homma, M. K.#, Motohashi, R.# and Ohtsubo, H. (2013). Japan's lagging gender equality. Science 340(6131): 428-430.(#Equal contribution)
  7. Motohashi, R., Rodiger, A., Agne, B., Baerenfaller, K. and Baginsky, S. (2012). Common and specific protein accumulation patterns in different albino/pale-green mutants reveals regulon organization at the proteome level. Plant Physiol 160(4): 2189-2201.
  8. Choi, J. H., Abe, N., Tanaka, H., Fushimi, K., Nishina, Y., Morita, A., Kiriiwa, Y., Motohashi, R., Hashizume, D., Koshino, H. and Kawagishi, H. (2010). Plant-growth regulator, imidazole-4-carboxamide, produced by the fairy ring forming fungus Lepista sordida. J Agric Food Chem 58(18): 9956-9959.
  9. Choi, J. H., Fushimi, K., Abe, N., Tanaka, H., Maeda, S., Morita, A., Hara, M., Motohashi, R., Matsunaga, J., Eguchi, Y., Ishigaki, N., Hashizume, D., Koshino, H. and Kawagishi, H. (2010). Disclosure of the "fairy" of fairy-ring-forming fungus Lepista sordida. Chembiochem 11(10): 1373-1377. 
  10. Tanaka, R., Rothbart, M., Oka, S., Takabayashi, A., Takahashi, K., Shibata, M., Myouga, F., Motohashi, R., Shinozaki, K., Grimm, B. and Tanaka, A. (2010). LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis. Proc Natl Acad Sci U S A 107(38): 16721-16725.   
  11. Myouga, F., Akiyama, K., Motohashi, R., Kuromori, T., Ito, T., Iizumi, H., Ryusui, R., Sakurai, T. and Shinozaki, K. (2010). The Chloroplast Function Database: a large-scale collection of Arabidopsis Ds/Spm- or T-DNA-tagged homozygous lines for nuclear-encoded chloroplast proteins, and their systematic phenotype analysis. Plant J 61(3): 529-542.
  12. Okuda, K., Hammani, K., Tanz, S. K., Peng, L., Fukao, Y., Myouga, F., Motohashi, R., Shinozaki, K., Small, I. and Shikanai, T. (2010). The pentatricopeptide repeat protein OTP82 is required for RNA editing of plastid ndhB and ndhG transcripts. Plant J 61(2): 339-349.
  13. Okuda, K., Chateigner-Boutin, A. L., Nakamura, T., Delannoy, E., Sugita, M., Myouga, F., Motohashi, R., Shinozaki, K., Small, I. and Shikanai, T. (2009). Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. Plant Cell 21(1): 146-156.
  14.  Myouga, F., Hosoda, C., Umezawa, T., Iizumi, H., Kuromori, T., Motohashi, R., Shono, Y., Nagata, N., Ikeuchi, M. and Shinozaki, K. (2008). A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis. Plant Cell 20(11): 3148-3162.
  15. Shimizu, H., Peng, L., Myouga, F., Motohashi, R., Shinozaki, K. and Shikanai, T. (2008). CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. Plant Cell Physiol 49(5): 835-842.
  16. Okuda, K., Myouga, F., Motohashi, R., Shinozaki, K. and Shikanai, T. (2007). Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing. Proc Natl Acad Sci U S A 104(19): 8178-8183.
  17. Motohashi, R., Yamazaki, T., Myouga, F., Ito, T., Ito, K., Satou, M., Kobayashi, M., Nagata, N., Yoshida, S., Nagashima, A., Tanaka, K., Takahashi, S. and Shinozaki, K. (2007). Chloroplast ribosome release factor 1 (AtcpRF1) is essential for chloroplast development. Plant Mol Biol 64(5): 481-497.
  18. Ichikawa, T., Nakazawa, M., Kawashima, M., Iizumi, H., Kuroda, H., Kondou, Y., Tsuhara, Y., Suzuki, K., Ishikawa, A., Seki, M., Fujita, M., Motohashi, R., Nagata, N., Takagi, T., Shinozaki, K. and Matsui, M. (2006). The FOX hunting system: an alternative gain-of-function gene hunting technique. Plant J 48(6): 974-985.
  19. Myouga, F., Motohashi, R., Kuromori, T., Nagata, N. and Shinozaki, K. (2006). An Arabidopsis chloroplast-targeted Hsp101 homologue, APG6, has an essential role in chloroplast development as well as heat-stress response. Plant J 48(2): 249-260.
  20. Ito, T., Motohashi, R., Kuromori, T., Noutoshi, Y., Seki, M., Kamiya, A., Mizukado, S., Sakurai, T. and Shinozaki, K. (2005). A resource of 5,814 dissociation transposon-tagged and sequence-indexed lines of Arabidopsis transposed from start loci on chromosome 5. Plant Cell Physiol 46(7): 1149-1153.
3 Protocols published
Hydroponic Culture of ‘Micro-Tom’ Tomato
Authors:  Reiko Motohashi, Harumi Enoki, Chikako Fukazawa and Yoshikazu Kiriiwa, date: 10/05/2015, view: 3513, Q&A: 0
We use ‘Micro-Tom’ to study tomato fruit ripening and development mechanisms. ‘Micro-Tom’ is suitable for cultivation and experiments due to its small size of 10 to 20 cm in height and short life cycle of 3 months. There is also an abundance of ...
Chlorophyll Fluorescence Measurements in Arabidopsis Plants Using a Pulse-amplitude-modulated (PAM) Fluorometer
Authors:  Reiko Motohashi and Fumiyoshi Myouga, date: 05/05/2015, view: 5758, Q&A: 0
In this protocol, to analyze PSII activity in photosynthesis, we measure the Fv/Fm (Fv=Fm ± Fo) value (Fo and Fm are the minimum and maximum values of chlorophyll fluorescence of dark-adapted leaves, respectively). Fv/Fm is a reliable marker of ...