Pedro Prudêncio Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Portugal
2 protocols
Jihyun Kim Bio-Protocol
5 protocols

Modesto Redrejo-Rodriguez Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
9 protocols

Leonardo Gaston Guilgur
  • Instituto Gulbenkian de Ciencia, Portugal
Research focus
  • Developmental biology
  • 2 Author merit
  • 6 Reviewer merit


Ph.D. in Molecular Biology and Biotechnology, Universidad de San Martín (UNSAM) Bue-nos Aires, Argentina, 2008

Current position

Post-doctoral fellow at Instituto Gulbenkian de Ciencia, Oeiras, Portugal


  1. Martinho, R. G., Guilgur, L. G. and Prudencio, P. (2015). How gene expression in fast-proliferating cells keeps pace. Bioessays 37(5): 514-524.
  2. Guilgur, L. G., Prudencio, P., Sobral, D., Liszekova, D., Rosa, A. and Martinho, R. G. (2014). Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3: e02181.
  3. Guilgur, L. G., Prudencio, P., Ferreira, T., Pimenta-Marques, A. R. and Martinho, R. G. (2012). Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells. Development 139(3): 503-513.
  4. Blasco, M., Fernandino, J. I., Guilgur, L. G., Strussmann, C. A., Somoza, G. M. and Vizziano-Cantonnet, D. (2010). Molecular characterization of cyp11a1 and cyp11b1 and their gene expression profile in pejerrey (Odontesthes bonariensis) during early gonadal development. Comp Biochem Physiol A Mol Integr Physiol 156(1): 110-118.
  5. Guilgur, L. G., Strussmann, C. A. and Somoza, G. M. (2009). mRNA expression of GnRH variants and receptors in the brain, pituitary and ovaries of pejerrey (Odontesthes bonariensis) in relation to the reproductive status. Fish Physiol Biochem 35(1): 157-166.
  6. Guilgur, L. G., Orti, G., Strobl-Mazzulla, P. H., Fernandino, J. I., Miranda, L. A. and So-moza, G. M. (2007). Characterization of the cDNAs encoding three GnRH forms in the pejerrey fish Odontesthes bonariensis (Atheriniformes) and the evolution of GnRH pre-cursors. J Mol Evol 64(6): 614-627.
  7. Miranda, L. A.; Strüssmann, C. A., Guilgur, L. G., Strobl-Mazzulla, P. H. and Somoza, G. M. (2007). Cloning of FSH-ß, LH-ß and glicoprotein hormone α subunits in pejerrey (Odontesthes bonariensis, Atheriniformes): expression profile and relationship with GnRHs expression and plasma sex steroid levels in male fish. J Fish Biol 71:1-19.
  8. Kah, O., Lethimonier, C., Somoza, G., Guilgur, L. G., Vaillant, C. and Lareyre, J. J. (2007). GnRH and GnRH receptors in metazoa: a historical, comparative, and evolutive perspective. Gen Comp Endocrinol 153(1-3): 346-364.
  9. Guilgur, L. G., Moncaut, N. P., Canario, A. V. and Somoza, G. M. (2006). Evolution of GnRH ligands and receptors in gnathostomata. Comp Biochem Physiol A Mol Integr Physiol 144(3): 272-283.
  10. Fernandino, J., Guilgur, L. and Somoza, G. (2006). Dmrt1 expression analysis during spermatogenesis in pejerrey, Odontesthes bonariensis. Fish Physiology and Biochemistry 32(3): 231-240.
  11. Somoza, G. M., Miranda, L. A., Guilgur, L. G. and Strobl-Mazzulla, P. H. (2006). Charac-terization of the brain-pituitary axis in pejerrey Odontesthes bonariensis. Biocell 30(1): 89-95.
  12. Mongiat, L. A., Fernandez, M. O., Lux-Lantos, V. A., Guilgur, L. G., Somoza, G. M. and Libertun, C. (2006). Experimental data supporting the expression of the highly con-served GnRH-II in the brain and pituitary gland of rats. Regul Pept 136(1-3): 50-57.
  13. Somoza, G. M., Miranda, L. A., Strobl-Mazzulla, P. and Guilgur, L. G. (2002). Gonado-tropin-releasing hormone (GnRH): from fish to mammalian brains. Cell Mol Neurobiol 22(5-6): 589-609.
2 Protocols published
Protein Extraction from Drosophila Embryos and Ovaries
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 5898, Q&A: 0
Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos ...
FLP/FRT Induction of Mitotic Recombination in Drosophila Germline
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 8040, Q&A: 0
The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces ...
6 Protocols reviewed
Gene Expression Analysis of Sorted Cells by RNA-seq in Drosophila Intestine
Authors:  Jun Chen, Jia Li, Huaiwei Huang and Rongwen Xi, date: 12/20/2016, view: 2206, Q&A: 0
RNA sequencing (RNA-seq) has become a popular method for profiling gene expression. Among many applications, one common purpose is to identify differentially expressed genes and pathways in different biological or pathological conditions. This ...
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Fat Turnover Assay in Drosophila
Author:  Subhash D. Katewa, date: 11/05/2016, view: 2368, Q&A: 0
Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of ...
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