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Coauthors
Fujio Kawamura Department of Life Science, College of Science, Rikkyo University, Japan
1 protocol

Shota Suzuki Biotechnology Research Center, The University of Tokyo, Japan
1 protocol

Genki Akanuma
  • Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Japan
Contributions
  • 1 Author merit

Education

Ph.D. in Molecular Genetics, Rikkyo University, Japan, 2007

Current position

Assistant Professor, Department of Life Science, Rikkyo University, Japan

Publications (since 2004)

  1. Akanuma, G., Kobayashi, A., Suzuki, S., Kawamura, F., Shiwa, Y., Watanabe, S., Yoshikawa, H., Hanai, R. and Ishizuka, M. (2014). Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium. J Bacteriol 196(22): 3820-3830.
  2. Suzuki, S., Tanigawa, O., Akanuma, G., Nanamiya, H., Kawamura, F., Tagami, K., Nomura, N., Kawabata, T. and Sekine, Y. (2014). Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2. Microbiology 160(Pt 6): 1040-1053.
  3. Yano, K., Wada, T., Suzuki, S., Tagami, K., Matsumoto, T., Shiwa, Y., Ishige, T., Kawaguchi, Y., Masuda, K., Akanuma, G., Nanamiya, H., Niki, H., Yoshikawa, H. and Kawamura, F. (2013). Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis. Microbiology 159(Pt 11): 2225-2236.
  4. Akanuma, G., Suzuki, S., Yano, K., Nanamiya, H., Natori, Y., Namba, E., Watanabe, K., Tagami, K., Takeda, T., Iizuka, Y., Kobayashi, A., Ishizuka, M., Yoshikawa, H. and Kawamura, F. (2013). Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis. J Gen Appl Microbiol 59(2): 105-117.
  5. Akanuma, G., Ishibashi, H., Miyagawa, T., Yoshizawa, R., Watanabe, S., Shiwa, Y., Yoshikawa, H., Ushio, K. and Ishizuka, M. (2013). EliA facilitates the induction of lipase expression by stearyl alcohol in Ralstonia sp. NT80. FEMS Microbiol Lett 339(1): 48-56.
  6. Akanuma, G., Nanamiya, H., Mouri, Y., Ishizuka, M. and Ohnishi, Y. (2012). Proteomic analysis of the Streptomyces griseus ribosomal fraction. Biosci Biotechnol Biochem 76(12): 2267-2274.
  7. Akanuma, G., Nanamiya, H., Natori, Y., Yano, K., Suzuki, S., Omata, S., Ishizuka, M., Sekine, Y. and Kawamura, F. (2012). Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. J Bacteriol 194(22): 6282-6291.
  8. Akanuma, G., Ueki, M., Ishizuka, M., Ohnishi, Y. and Horinouchi, S. (2011). Control of aerial mycelium formation by the BldK oligopeptide ABC transporter in Streptomyces griseus. FEMS Microbiol Lett 315(1): 54-62.
  9. Nanamiya, H., Sato, M., Masuda, K., Sato, M., Wada, T., Suzuki, S., Natori, Y., Katano, M., Akanuma, G. and Kawamura, F. (2010). Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation. Microbiology 156(Pt 10): 2944-2952.
  10. Akanuma, G., Hara, H., Ohnishi, Y. and Horinouchi, S. (2009). Dynamic changes in the extracellular proteome caused by absence of a pleiotropic regulator AdpA in Streptomyces griseus. Mol Microbiol 73(5): 898-912.
  11. Nakano, C., Ozawa, H., Akanuma, G., Funa, N. and Horinouchi, S. (2009). Biosynthesis of aliphatic polyketides by type III polyketide synthase and methyltransferase in Bacillus subtilis. J Bacteriol 191(15): 4916-4923.
  12. Natori, Y., Nanamiya, H., Akanuma, G., Kosono, S., Kudo, T., Ochi, K. and Kawamura, F. (2007). A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis. Mol Microbiol 63(1): 294-307.
  13. Koga, K., Ikegami, A., Nakasone, K., Murayama, R., Akanuma, G., Natori, Y., Nanamiya, H. and Kawamura, F. (2006). Construction of Bacillus subtilis strains carrying the transcriptional bgaB fusion with the promoter region of each rrn operon and their differential transcription during spore development. J Gen Appl Microbiol 52(2): 119-124.
  14. Akanuma, G., Habu, C., Natori, Y., Murayama, R., Nanamiya, H. and Kawamura, F. (2006). Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region. FEMS Microbiol Lett 258(2): 220-226.
  15. Akanuma, G., Nanamiya, H., Natori, Y., Nomura, N. and Kawamura, F. (2006). Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis. J Bacteriol 188(7): 2715-2720.
  16. Murayama, R., Akanuma, G., Makino, Y., Nanamiya, H. and Kawamura, F. (2004). Spontaneous transformation and its use for genetic mapping in Bacillus subtilis. Biosci Biotechnol Biochem 68(8): 1672-1680.
  17. Nanamiya, H. *, Akanuma, G. *, Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, S. M., Ochi, K. and Kawamura, F. (2004). Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome. Mol Microbiol 52(1): 273-283. (*The first two authors contributed equally to this work)
1 Protocol published
Purification of 70S Ribosomes from Bacillus subtilis
Authors:  Shota Suzuki, Genki Akanuma and Fujio Kawamura, date: 04/05/2015, view: 3515, Q&A: 0
The eubacterial ribosome (70S) is a macromolecular complex that is composed of a small (30S) subunit and a large (50S) subunit. The small subunit comprises the 16S ribosomal RNA (rRNA) and more than 20 ribosomal proteins (r-proteins), whereas the ...