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Coauthors
Helene Roelofs Medical Center, Leiden University, The Netherlands
2 protocols

C. Schrama Medical Center, Leiden University, The Netherlands
2 protocols

Reviewer
Sara M. Melief
  • Leiden University
Research focus
  • Stem cell
  • 2 Author merit
2 Protocols published
Monocyte-MSC Co-cultures
Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 4132, Q&A: 0
To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage ...
PBMC-MSC Co-cultures for Induction of Treg Generation
Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 4067, Q&A: 0
To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were ...