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Coauthors
Cheng-Hsun Ho Agricultural Biotechnology Research Center of Academia Sinica
1 protocol

Roberto De Michele CNR-IGV Institute of Biosciences and Bioresources
1 protocol

Guido Grossmann Universität Heidelberg
1 protocol

Cindy Cooke Carnegie Institution for Science
1 protocol

Wolf B. Frommer
  • Department of Plant Biology, Carnegie Science, USA
Contributions
  • 2 Author merit
2 Protocols published
Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Activity Sensors in Yeast Cultures
Authors:  Cheng-Hsun Ho and Wolf B. Frommer, date: 02/05/2016, view: 2758, Q&A: 0
This protocol describes the methods used to engineer and deploy genetically encoded fluorescence activity reporters for nitrate and peptide transporter activity in yeast cells. Fusion of the dual-affinity nitrate transceptor CHL1/AtNRT1.1/AtNPF6.3 ...
Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors
Authors:  Cindy Ast, Wolf B. Frommer, Guido Grossmann and Roberto De Michele, date: 01/05/2015, view: 3836, Q&A: 0
AmTracs are the first example of “activity sensors”, since they report the activity of ammonium transporters by means of fluorescence readout in vivo (De Michele et al., 2013). AmTracs are based on a single fluorescent protein, a ...