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Coauthors
Wolf Frommer Department of Plant Biology, Carnegie Science, USA
2 protocols

Roberto De Michele CNR-IGV Institute of Biosciences and Bioresources
1 protocol

Guido Grossmann Universität Heidelberg
1 protocol
Coreviewers
Arsalan Daudi University of California
153 protocols

Fanglian He University of Pennsylvania
205 protocols

Feng Li Huazhong Agricultural University
12 protocols

Igor Cesarino University of São Paulo
8 protocols

Reviewer
Cindy Ast Cooke
  • Post-Doc, Carnegie Institution for Science
Research focus
  • Biophysics
  • Fluorescent Proteins, Biosensors, Spectroscopy, Microscopy
  • 1 Author merit

Education

PhD, University of Potsdam

Lab information

Frommer Lab

Publications

Ast, C., Foret, J., Oltrogge, L.M., De Michele, R., Kleist, T.J., Ho, C.-H., and Frommer, W.B. Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun (2017).
Ast, C., De Michele, R., Kumke, M. U. & Frommer, W. B. Single-fluorophore membrane transport activity sensors with dual-emission read-out. eLife 4, (2015).
Ast, C., Frommer, W. B., Grossmann, G. & De Michele, R. Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors. Bio-Protoc. 5, e1372 (2015).
Ast, C. & Draaijer, A. in Low-Oxygen Stress in Plants (eds. Dongen, J. T. van & Licausi, F.) 397–417 (Springer Vienna, 2014).
Haupt, J. et al. ACVR1 p.Q207E causes classic fibrodysplasia ossificans progressiva and is functionally distinct from the engineered constitutively active ACVR1 p.Q207D variant. Hum. Mol. Genet. 23, 5364–5377 (2014).
De Michele, R. et al. Fluorescent sensors reporting the activity of ammonium transceptors in live cells. eLife 2, e00800 (2013).
Wang, Q. et al. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization. Proc. Natl. Acad. Sci. U. S. A. 110, 13204–13209 (2013).
Ast, C., Schmälzlin, E., Löhmannsröben, H.-G. & van Dongen, J. T. Optical oxygen micro- and nanosensors for plant applications. Sensors 12, 7015–7032 (2012).
Hill, D. et al. Size determination of quantum dots with fluorescence correlation spectroscopy. in (eds. Parak, W. J., Yamamoto, K. & Osinski, M.) 790904 (2011). doi:10.1117/12.874660
Shen, Q. et al. The fibrodysplasia ossificans progressiva R206H ACVR1 mutation activates BMP-independent chondrogenesis and zebrafish embryo ventralization. J. Clin. Invest. 119, 3462–3472 (2009).

1 Protocol published
Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors
Authors:  Cindy Ast, Wolf B. Frommer, Guido Grossmann and Roberto De Michele, date: 01/05/2015, view: 3959, Q&A: 0
AmTracs are the first example of “activity sensors”, since they report the activity of ammonium transporters by means of fluorescence readout in vivo (De Michele et al., 2013). AmTracs are based on a single fluorescent protein, a ...
11 Protocols reviewed
Plasma Membrane Preparation from Lilium davidii and Oryza sativa Mature and Germinated Pollen
Authors:  Ning Yang, Bing Han, Lingtong Liu, Hao Yang and Tai Wang, date: 05/20/2017, view: 1313, Q&A: 0
Pollen germination is an excellent process to study cell polarity establishment. During this process, the tip-growing pollen tube will start elongating. The plasma membrane as the selectively permeable barrier that separates the inner and outer cell ...
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Indirect Immunofluorescence Assay in Chlamydomonas reinhardtii
Authors:  Takashi Yamano and Hideya Fukuzawa, date: 07/05/2016, view: 2837, Q&A: 0
Determining the protein localization is essential to elucidate its in vivo function. Fluorescence-tagged proteins are widely used for it, but it is sometimes difficult to express tagged proteins in Chlamydomonas. Alternatively, ...
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