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Coauthors
Frances Separovic School of Chemistry, The University of Melbourne, Australia
2 protocols

Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol
Coreviewers
Arsalan Daudi Department of Plant Pathology, University of California, Davis, CA, USA
137 protocols

Ching Yao Yang New York University
2 protocols

Daniel Kraus 1st Department of Medicine, Würzburg University Hospital
5 protocols

Fernanda Salvato Institute of Biology, State University of Campinas
5 protocols

Marc-Antoine Sani
  • Research Fellow, School of Chemistry, The University of Melbourne, Australia
Research focus
  • Biophysics
  • Structural biology, NMR spectroscopy, lipid-protein interactions
Contributions
  • 2 Author merit
  • 3 Reviewer merit
  • 5 Editor merit

Lab information

Group leader: Frances Separovic

Publications

https://scholar.google.com.au/citations?hl=en&user=Er-RAsQAAAAJ&view_op=list_works&sortby=pubdate

2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 3117, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...
3 Protocols reviewed
Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-γATP
Authors:  Michael J. Munson and Ian G. Ganley, date: 08/20/2016, view: 1952, Q&A: 0
VPS34 is the only class III phosphatidylinositol-3-kinase (PI3K) in mammalian cells and produces the vast majority of cellular phosphatidylinositol-3-phosphate [PI(3)P]. PI(3)P is a key signalling lipid that plays many membrane trafficking roles in ...
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Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology
Authors:  Reto Horst and Kurt Wüthrich, date: 07/20/2015, view: 2014, Q&A: 0
Reconstitution of integral membrane proteins (IMP) in aqueous solutions of detergent micelles has been extensively used in structural biology, using either X-ray crystallography or NMR in solution. Further progress could be achieved by establishing ...
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5 Protocols edited
Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Author:  Marina Verkhovskaya, date: 05/05/2017, view: 707, Q&A: 0
The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by ...
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Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies
Authors:  Megan Sjodt and Robert T. Clubb, date: 04/05/2017, view: 957, Q&A: 0
Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach ...
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