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Coauthors
Frances Separovic The University of Melbourne
2 protocols

Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol
Coreviewers
Alka Mehra Institute of Genomics and Integrative Biology
14 protocols

Arsalan Daudi University of California
137 protocols

Ashish Sethi University of Melbourne
1 protocol

Ching Yao Yang New York University
3 protocols

Editor
Marc-Antoine Sani
  • Research Fellow, University of Melbourne
Research focus
  • Biophysics
  • Structural biology, NMR spectroscopy, lipid-protein interactions, biophysics
Contributions
  • 2 Author merit
  • 3 Reviewer merit
  • 5 Editor merit

Education

PhD, University of Bordeaux / Umea University, 2008

Lab information

Group leader: Frances Separovic

Publications

https://scholar.google.com.au/citations?hl=en&user=Er-RAsQAAAAJ&view_op=list_works&sortby=pubdate

2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 3446, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...
3 Protocols reviewed
Bioelectrospray Methodology for Dissection of the Host-pathogen Interaction in Human Tuberculosis
Authors:  Liku B Tezera, Magdalena K Bielecka and Paul T Elkington, date: 07/20/2017, view: 959, Q&A: 0
Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where ...
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Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-γATP
Authors:  Michael J. Munson and Ian G. Ganley, date: 08/20/2016, view: 2286, Q&A: 0
VPS34 is the only class III phosphatidylinositol-3-kinase (PI3K) in mammalian cells and produces the vast majority of cellular phosphatidylinositol-3-phosphate [PI(3)P]. PI(3)P is a key signalling lipid that plays many membrane trafficking roles in ...
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5 Protocols edited
Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Author:  Marina Verkhovskaya, date: 05/05/2017, view: 1026, Q&A: 0
The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by ...
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Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies
Authors:  Megan Sjodt and Robert T. Clubb, date: 04/05/2017, view: 1387, Q&A: 0
Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach ...
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