Miwa Ohnishi Department of Biology, Kobe University, Graduate School of Science, Japan
2 protocols

Yuko Kurita Ryukoku University
2 protocols

Satomi Kanno Department of Biology, Kobe University, Graduate School of Science, Japan
2 protocols

Tetsuro Mimura
  • Department of Biology, Kobe University, Graduate School of Science, Japan
  • 2 Author merit


Ph.D. in Botany, Department of Biology, Graduate School of Science, the University of Tokyo, 1984

Current position

Professor, Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan

Publications (since 2004)

  1. Mimura, T., Mimura, M., Kobayashi, D., Komiyama, C., Sekimoto, H., Miyamoto, M. and Kitamura, A. (2014). Radioactive pollution and accumulation of radionuclides in wild plants in Fukushima. J Plant Res 127(1): 5-10.
  2. Kurita, Y., Baba, K., Ohnishi, M., Anegawa, A., Shichijo, C., Kosuge, K., Fukaki, H. and Mimura, T. (2014). Establishment of a shortened annual cycle system; a tool for the analysis of annual re-translocation of phosphorus in the deciduous woody plant (Populus alba L.). J Plant Res 127(4): 545-551.
  3. Kosuge, K., Iida, S., Katou, K. and Mimura, T. (2013). Circumnutation on the water surface: female flowers of Vallisneria. Sci Rep 3: 1133.
  4. Kadohama, N., Goh, T., Ohnishi, M., Fukaki, H., Mimura, T. and Suzuki, Y. (2013). Sudden collapse of vacuoles in Saintpaulia sp. palisade cells induced by a rapid temperature decrease. PLoS One 8(2): e57259.
  5. Nagai, M., Ohnishi, M., Uehara, T., Yamagami, M., Miura, E., Kamakura, M., Kitamura, A., Sakaguchi, S., Sakamoto, W., Shimmen, T., Fukaki, H., Reid, R. J., Furukawa, A. and Mimura, T. (2013). Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. Plant Cell Environ 36(10): 1826-1837.
  6. Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Fujiwara, M., Song, C., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Hayashi, F., Fukaki, H., Maeshima, M. and Mimura, T. (2013). Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: local distribution of vacuolar membrane proteins. Plant Cell Physiol 54(10): 1571-1584.
  7. Goh, T., Joi, S., Mimura, T. and Fukaki, H. (2012). The establishment of asymmetry in Arabidopsis lateral root founder cells is regulated by LBD16/ASL18 and related LBD/ASL proteins. Development 139(5): 883-893.
  8. Oikawa, A., Matsuda, F., Kikuyama, M., Mimura, T. and Saito, K. (2011). Metabolomics of a single vacuole reveals metabolic dynamism in an alga Chara australis. Plant Physiol 157(2): 544-551.
  9. Kuwano, M., Mimura, T., Takaiwa, F. and Yoshida, K. T. (2009). Generation of stable 'low phytic acid' transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene (RINO1) using the 18-kDa oleosin promoter. Plant Biotechnol J 7(1): 96-105.
  10. Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, M. H., Morita, M. T., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H. and Mimura, T. (2009). Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. Plant Cell Physiol 50(12): 2023-2033.
  11. Mitsuhashi, N., Kondo, M., Nakaune, S., Ohnishi, M., Hayashi, M., Hara-Nishimura, I., Richardson, A., Fukaki, H., Nishimura, M. and Mimura, T. (2008). Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis. J Exp Bot 59(11): 3069-3076.
  12. Kawachi, M., Kobae, Y., Mimura, T. and Maeshima, M. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J Biol Chem 283(13): 8374-8383.
  13. Ohnishi, M., Mimura, T., Tsujimura, T., Mitsuhashi, N., Washitani-Nemoto, S., Maeshima, M. and Martinoia, E. (2007). Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status. Planta 225(3): 711-718.
  14. Mitsuhashi, N., Ohnishi, M., Sekiguchi, Y., Kwon, Y. U., Chang, Y. T., Chung, S. K., Inoue, Y., Reid, R. J., Yagisawa, H. and Mimura, T. (2005). Phytic acid synthesis and vacuolar accumulation in suspension-cultured cells of Catharanthus roseus induced by high concentration of inorganic phosphate and cations. Plant Physiol 138(3): 1607-1614.
  15. Sekiguchi, Y., Mitsuhashi, N., Kokaji, T., Miyakoda, H. and Mimura, T. (2005). Development of a comprehensive analytical method for phosphate metabolites in plants by ion chromatography coupled with tandem mass spectrometry. J Chromatogr A 1085(1): 131-136.
  16. Kubo, M., Udagawa, M., Nishikubo, N., Horiguchi, G., Yamaguchi, M., Ito, J., Mimura, T., Fukuda, H. and Demura, T. (2005). Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev 19(16): 1855-1860.
  17. Sekiguchi, Y., Mitsuhashi, N., Inoue, Y., Yagisawa, H. and Mimura, T. (2004). Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection. J Chromatogr A 1039(1-2): 71-76.
  18. Shimaoka, T., Ohnishi, M., Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K. and Mimura, T. (2004). Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Plant Cell Physiol 45(6): 672-683.
2 Protocols published
Autoradiography of Pi Distribution in Barley Seedlings
Authors:  Satomi Kanno, Yuko Kurita, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 3222, Q&A: 0
Phosphorus-32 and Phosphorus-33 are radioisotopes of phosphorus. These isotopes are used to trace ionic phosphorus and phosphorus compounds. This protocol is used to follow the movement of inorganic phosphate (PO43-) from a ...
Extraction of Ions from Leaf Sections
Authors:  Yuko Kurita, Satomi Kanno, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 2713, Q&A: 0
The concentration of ions in plant cells and tissues is an important factor to determine their functions and conditions. Here, we describe the method to extract ions from leaf sections for measurements with an ion chromatogram. This method is ...