Tetsuro Mimura Department of Biology, Kobe University, Graduate School of Science, Japan
2 protocols

Yuko Kurita Ryukoku University
2 protocols

Satomi Kanno Department of Biology, Kobe University, Graduate School of Science, Japan
2 protocols

Miwa Ohnishi
  • Department of Biology, Kobe University, Graduate School of Science, Japan
  • 2 Author merit


Ph .D. in Biology, Department of Biology, Graduate School of Science, Kobe University, 2007

Current position

Assistant Professor, Department of Biology, Graduate School of Science, Kobe University


  1. Kurita, Y., Baba, K., Ohnishi, M., Anegawa, A., Shichijo, C., Kosuge, K., Fukaki, H. and Mimura, T. (2014). Establishment of a shortened annual cycle system; a tool for the analysis of annual re-translocation of phosphorus in the deciduous woody plant (Populus alba L.). J Plant Res 127(4): 545-551.
  2. Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Fujiwara, M., Song, C., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Hayashi, F., Fukaki, H., Maeshima, M. and Mimura, T. (2013). Studies on vacuolar membrane microdomains isolated from Arabidopsis suspension-cultured cells: local distribution of vacuolar membrane proteins. Plant Cell Physiol 54(10): 1571-1584.
  3. Nagai, M., Ohnishi, M., Uehara, T., Yamagami, M., Miura, E., Kamakura, M., Kitamura, A., Sakaguchi, S., Sakamoto, W., Shimmen, T., Fukaki, H., Reid, R. J., Furukawa, A. and Mimura, T. (2013). Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. Plant Cell Environ 36(10): 1826-1837.
  4. Kadohama, N., Goh, T., Ohnishi, M., Fukaki, H., Mimura, T. and Suzuki, Y. (2013). Sudden collapse of vacuoles in Saintpaulia sp. palisade cells induced by a rapid temperature decrease. PLoS One 8(2): e57259.
  5. Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, M. H., Morita, M. T., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H. and Mimura, T. (2009). Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. Plant Cell Physiol 50(12): 2023-2033.
  6. Mitsuhashi, N., Kondo, M., Nakaune, S., Ohnishi, M., Hayashi, M., Hara-Nishimura, I., Richardson, A., Fukaki, H., Nishimura, M. and Mimura, T. (2008). Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis. J Exp Bot 59(11): 3069-3076.
  7. Ohnishi, M., Mimura, T., Tsujimura, T., Mitsuhashi, N., Washitani-Nemoto, S., Maeshima, M. and Martinoia, E. (2007). Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status. Planta 225(3): 711-718.
  8. Mitsuhashi, N., Ohnishi, M., Sekiguchi, Y., Kwon, Y. U., Chang, Y. T., Chung, S. K., Inoue, Y., Reid, R. J., Yagisawa, H. and Mimura, T. (2005). Phytic acid synthesis and vacuolar accumulation in suspension-cultured cells of Catharanthus roseus induced by high concentration of inorganic phosphate and cations. Plant Physiol 138(3): 1607-1614.
  9. Kobae, Y., Uemura, T., Sato, M. H., Ohnishi, M., Mimura, T., Nakagawa, T. and Maeshima, M. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol 45(12): 1749-1758.
  10. Kataoka, T., Watanabe-Takahashi, A., Hayashi, N., Ohnishi, M., Mimura, T., Buchner, P., Hawkesford, M. J., Yamaya, T. and Takahashi, H. (2004). Vacuolar sulfate transporters are essential determinants controlling internal distribution of sulfate in Arabidopsis. Plant Cell 16(10): 2693-2704.
  11. Shimaoka, T., Ohnishi, M., Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K. and Mimura, T. (2004). Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Plant Cell Physiol 45(6): 672-683.
  12. Mimura, T., Kura-Hotta, M., Tsujimura, T., Ohnishi, M., Miura, M., Okazaki, Y., Mimura, M., Maeshima, M. and Washitani-Nemoto, S. (2003). Rapid increase of vacuolar volume in response to salt stress. Planta 216(3): 397-402.
2 Protocols published
Autoradiography of Pi Distribution in Barley Seedlings
Authors:  Satomi Kanno, Yuko Kurita, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 3128, Q&A: 0
Phosphorus-32 and Phosphorus-33 are radioisotopes of phosphorus. These isotopes are used to trace ionic phosphorus and phosphorus compounds. This protocol is used to follow the movement of inorganic phosphate (PO43-) from a ...
Extraction of Ions from Leaf Sections
Authors:  Yuko Kurita, Satomi Kanno, Miwa Ohnishi and Tetsuro Mimura, date: 07/05/2014, view: 2623, Q&A: 0
The concentration of ions in plant cells and tissues is an important factor to determine their functions and conditions. Here, we describe the method to extract ions from leaf sections for measurements with an ion chromatogram. This method is ...