Owen Rowland Biology, Carleton University, Canada
3 protocols

Sollapura Vishwanath Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Canada
2 protocols

Camille Delude Laboratoire de Biogenèse Membranaire, UMR 5200, CNRS – Université de Bordeaux, INRA Bordeaux Aquitaine, France
1 protocol

Frédéric Domergue
  • Laboratoire de Biogenèse Membranaire, UMR 5200, CNRS - Université de Bordeaux, France
  • 2 Author merit


Ph.D. in Plant Biochemistry, Université de Bordeaux II, Bordeaux, France, 1997

Current position

Research Associate (CNRS - CR1)/Surface Lipids Team Coordinator, Laboratoire de Biogenèse Membranaire, CNRS - UMR 5200/Université de Bordeaux, France


  1. Petit, J., Bres, C., Just, D., Garcia, V., Mauxion, J. P., Marion, D., Bakan, B., Joubes, J., Domergue, F. and Rothan, C. (2014). Analyses of tomato fruit brightness mutants uncover both cutin-deficient and cutin-abundant mutants and a new hypomorphic allele of GDSL lipase. Plant Physiol 164(2): 888-906.
  2. Pulsifer, I. P., Lowe, C., Narayaran, S. A., Busuttil, A. S., Vishwanath, S. J., Domergue, F. and Rowland, O. (2014). Acyl-lipid thioesterase1-4 from Arabidopsis thaliana form a novel family of fatty acyl-acyl carrier protein thioesterases with divergent expression patterns and substrate specificities. Plant Mol Biol 84(4-5): 549-563.
  3. Vishwanath, S. J., Kosma, D. K., Pulsifer, I. P., Scandola, S., Pascal, S., Joubès, J., Dittrich-Domergue, F., Lessire, R., Rowland, O. and Domergue, F. (2013). Suberin-Associated Fatty Alcohols in Arabidopsis: Distributions in Roots and Contributions to Seed Coat Barrier Properties. Plant physiology 163(3): 1118-1132.
  4. Chacon, M. G., Fournier, A. E., Tran, F., Dittrich-Domergue, F., Pulsifer, I. P., Domergue, F. and Rowland, O. (2013). Identification of amino acids conferring chain length substrate specificities on fatty alcohol-forming reductases FAR5 and FAR8 from Arabidopsis thaliana. J Biol Chem 288(42): 30345-30355.
  5. Le Provost, G.*, Domergue, F.*, Lalanne, C., Ramos Campos, P., Grosbois, A., Bert, D., Meredieu, C., Danjon, F., Plomion, C. and Gion, J. M. (2013). Soil water stress affects both cuticular wax content and cuticle-related gene expression in young saplings of maritime pine (Pinus pinaster Ait). BMC Plant Biol 13: 95 (*Co-first author)
  6. Paquet, V. E., Lessire, R., Domergue, F., Fouillen, L., Filion, G., Sedighi, A. and Charette, S. J. (2013). Lipid composition of multilamellar bodies secreted by Dictyostelium discoideum reveals their amoebal origin. Eukaryot Cell 12(10): 1326-1334.
  7. Pascal, S., Bernard, A., Sorel, M., Pervent, M., Vile, D., Haslam, R. P., Napier, J. A., Lessire, R., Domergue, F. and Joubes, J. (2013). The Arabidopsis cer26 mutant, like the cer2 mutant, is specifically affected in the very long chain fatty acid elongation process. Plant J 73(5): 733-746.
  8. Bernard, A., Domergue, F., Pascal, S., Jetter, R., Renne, C., Faure, J. D., Haslam, R. P., Napier, J. A., Lessire, R. and Joubes, J. (2012). Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex. Plant Cell 24(7): 3106-3118.
  9. Rowland, O. and Domergue, F. (2012). Plant fatty acyl reductases: enzymes generating fatty alcohols for protective layers with potential for industrial applications. Plant Sci 193-194: 28-38.
  10. Cacas, J. L., Melser, S., Domergue, F., Joubes, J., Bourdenx, B., Schmitter, J. M. and Mongrand, S. (2012). Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS. Anal Bioanal Chem 403(9): 2745-2755.
  11. Doan, T. T., Domergue, F., Fournier, A. E., Vishwanath, S. J., Rowland, O., Moreau, P., Wood, C. C., Carlsson, A. S., Hamberg, M. and Hofvander, P. (2012). Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana. Biochim Biophys Acta 1821(9): 1244-1255.
  12. Bourdenx, B., Bernard, A., Domergue, F., Pascal, S., Leger, A., Roby, D., Pervent, M., Vile, D., Haslam, R. P., Napier, J. A., Lessire, R. and Joubes, J. (2011). Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiol 156(1): 29-45.
  13. Bure, C., Cacas, J. L., Wang, F., Gaudin, K., Domergue, F., Mongrand, S. and Schmitter, J. M. (2011). Fast screening of highly glycosylated plant sphingolipids by tandem mass spectrometry. Rapid Commun Mass Spectrom 25(20): 3131-3145.
  14. Javelle, M., Vernoud, V., Depege-Fargeix, N., Arnould, C., Oursel, D., Domergue, F., Sarda, X. and Rogowsky, P. M. (2010). Overexpression of the epidermis-specific homeodomain-leucine zipper IV transcription factor Outer Cell Layer1 in maize identifies target genes involved in lipid metabolism and cuticle biosynthesis. Plant Physiol 154(1): 273-286.
  15. Domergue, F., Vishwanath, S. J., Joubes, J., Ono, J., Lee, J. A., Bourdon, M., Alhattab, R., Lowe, C., Pascal, S., Lessire, R. and Rowland, O. (2010). Three Arabidopsis fatty acyl-coenzyme A reductases, FAR1, FAR4, and FAR5, generate primary fatty alcohols associated with suberin deposition. Plant Physiol 153(4): 1539-1554.
  16. LiBach, L., Michaelson, L. V., Haslam, R., Bellec, Y., Gissot, L., Marion, J., Da Costa, M., Boutin, J. P., Miquel, M., Tellier, F., Domergue, F., Markham, J. E., Beaudoin, F., Napier, J. A. and Faure, J. D. (2008). The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development. Proc Natl Acad Sci U S A 105(38): 14727-14731.
  17. Raffaele, S., Vailleau, F., Leger, A., Joubes, J., Miersch, O., Huard, C., Blee, E., Mongrand, S., Domergue, F. and Roby, D. (2008). A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis. Plant Cell 20(3): 752-767.
  18. Joubes, J., Raffaele, S., Bourdenx, B., Garcia, C., Laroche-Traineau, J., Moreau, P., Domergue, F. and Lessire, R. (2008). The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling. Plant Mol Biol 67(5): 547-566.
  19. Domergue, F., Abbadi, A., Zahringer, U., Moreau, H. and Heinz, E. (2005). In vivo characterization of the first acyl-CoA Delta6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem J 389(Pt 2): 483-490.
  20. Domergue, F., Abbadi, A. and Heinz, E. (2005). Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trends Plant Sci 10(3): 112-116.
  21. Abbadi, A., Domergue, F., Bauer, J., Napier, J. A., Welti, R., Zahringer, U., Cirpus, P. and Heinz, E. (2004). Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16(10): 2734-2748.
  22. Meyer, A., Kirsch, H., Domergue, F., Abbadi, A., Sperling, P., Bauer, J., Cirpus, P., Zank, T. K., Moreau, H., Roscoe, T. J., Zahringer, U. and Heinz, E. (2004). Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J Lipid Res 45(10): 1899-1909.
  23. Domergue, F., Abbadi, A., Ott, C., Zank, T. K., Zahringer, U. and Heinz, E. (2003). Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. J Biol Chem 278(37): 35115-35126.
  24. Domergue, F., Spiekermann, P., Lerchl, J., Beckmann, C., Kilian, O., Kroth, P. G., Boland, W., Zahringer, U. and Heinz, E. (2003). New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial and microsomal delta12-fatty acid desaturases. Plant Physiol 131(4): 1648-1660.
  25. Drexler, H., Spiekermann, P., Meyer, A., Domergue, F., Zank, T., Sperling, P., Abbadi, A. and Heinz, E. (2003). Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. J Plant Physiol 160(7): 779-802.
  26. Domergue, F., Lerchl, J., Zahringer, U. and Heinz, E. (2002). Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis. Eur J Biochem 269(16): 4105-4113.
  27. Abbadi, A., Domergue, F., Meyer, A., Riedel, K., Sperling, P., Zank, T. K. and Heinz, E. (2001). Transgenic oilseeds as sustainable source of nutritionally relevant C20 and C22 polyunsaturated fatty acids? European journal of lipid science and technology 103(2): 106-113.
  28. Metz, J. G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K., Domergue, F., Yamada, A., Yazawa, K., Knauf, V. and Browse, J. (2001). Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293(5528): 290-293.
  29. Domergue, F., Helms, G. L., Prusky, D. and Browse, J. (2000). Antifungal compounds from idioblast cells isolated from avocado fruits. Phytochemistry 54(2): 183-189.
  30. Domergue, F., Chevalier, S., Creach, A., Cassagne, C. and Lessire, R. (2000). Purification of the acyl-CoA elongase complex from developing rapeseed and characterization of the 3-ketoacyl-CoA synthase and the 3-hydroxyacyl-CoA dehydratase. Lipids 35(5): 487-494.
  31. Domergue, F., Chevalier, S., Santarelli, X., Cassagne, C. and Lessire, R. (1999). Evidence that oleoyl-CoA and ATP-dependent elongations coexist in rapeseed (Brassica napus L.). Eur J Biochem 263(2): 464-470.
  32. Lessire, R., Domergue, F., Spinner, C., Lucet-Levannier, K., Lellouche, J.-P., Mioskowski, C. and Cassagne, C. (1998). Dehydration of 3-hydroxy icosanoyl-CoA and reduction of (E) 2, 3 icosenoyl-CoA are required for elongation by leek microsomal elongase (s). Plant Physiology and Biochemistry 36(3): 205-211.
  33. Barret, P., Delourme, R., Renard, M., Domergue, F., Lessire, R., Delseny, M. and Roscoe, T. (1998). A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid. Theoretical and Applied Genetics 96(2): 177-186.
  34. Santarelli, X., Domergue, F., Clofent-Sanchez, G., Dabadie, M., Grissely, R. and Cassagne, C. (1998). Characterization and application of new macroporous membrane ion exchangers. J Chromatogr B Biomed Sci Appl 706(1): 13-22.
2 Protocols published
Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
Authors:  Camille Delude, Sollapura J. Vishwanath, Owen Rowland and Frédéric Domergue, date: 06/20/2017, view: 624, Q&A: 0
Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are ...
Seed Coat Permeability Test: Tetrazolium Penetration Assay
Authors:  Sollapura J. Vishwanath, Frédéric Domergue and Owen Rowland, date: 07/05/2014, view: 4381, Q&A: 1
Seed coat permeability is important to study as it plays significant roles in seed dormancy, germination, and protection from pathogens. Here we describe a commonly used seed coat permeability test known as the tetrazolium penetration assay with a ...