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Coauthors
Tobias Spielmann Parasitology Section, Bernhard Nocht Institute for Tropical Medicine, Germany
2 protocols

Silke Retzlaff Parasitology Section, Bernhard Nocht Institute for Tropical Medicine, Germany
1 protocol

Arlett Heiber
  • Parasitology Section, Bernhard Nocht Institute for Tropical Medicine, Germany
Contributions
  • 2 Author merit
2 Protocols published
Immuno-EM Analysis of PF13_0191-GFP Expressing Parasites
Authors:  Arlett Heiber, Silke Retzlaff and Tobias Spielmann, date: 06/05/2014, view: 3170, Q&A: 0
This protocol was used to prepare pre-embedding samples of Plasmodium falciparum blood stage parasites that overexpressed the parasite protein PF13_0191 tagged with GFP. Using GFP-specific antibodies and Protein A-Gold the localisation of ...
Preparation of Parasite Protein Extracts and Western Blot Analysis
Authors:  Arlett Heiber and Tobias Spielmann, date: 06/05/2014, view: 8672, Q&A: 0
In order to prepare protein extracts of Plasmodium falciparum blood stages for western blot analysis, infected red blood cells (iRBC) need to be separated from uninfected red blood cells (uRBC) which make up the bulk of the parasite ...