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Coauthors
Guang-Yuh Jauh Institute of Plant and Microbial Biology, Academia Sinica, Taiwan
3 protocols

Christopher Makaroff Department of Chemistry and Biochemistry, Miami University, USA
1 protocol

Pablo Bolanos-Villegas University of Costa Rica
1 protocol

Xiaohui Yang
  • Department of Chemistry and Biochemistry, Miami University, USA
  • 1 Author merit

Education

Ph.D. in Plant Molecular Biology, National University of Singapore, 1998

Current position

Adjunct Associate Professor, Department Chemistry and Biochemistry, Miami University, Oxford, OH 45056

Publications

  1. Bolaños-Villegas, P., Yang, X., Wang, H. J., Juan, C. T., Chuang, M. H., Makaroff, C. A. and Jauh, G. Y. (2013). Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis. Plant J 75(6): 927-940.
  2. Yang, X., Yuan, L. and Makaroff, C. A. (2013). Immunolocalization protocols for visualizing meiotic proteins in Arabidopsis thaliana: method 3. Methods Mol Biol 990: 109-118.
  3. Yuan, L., Yang, X., Ellis, J. L., Fisher, N. M. and Makaroff, C. A. (2012). The Arabidopsis SYN3 cohesin protein is important for early meiotic events. Plant J 71(1): 147-160.
  4. Yang, X., Boateng, K. A., Yuan, L., Wu, S., Baskin, T. I. and Makaroff, C. A. (2011). The radially swollen 4 separase mutation of Arabidopsis thaliana blocks chromosome disjunction and disrupts the radial microtubule system in meiocytes. PLoS One 6(4): e19459.
  5. Yuan, L., Yang, X. and Makaroff, C. A. (2011). Plant cohesins, common themes and unique roles. Curr Protein Pept Sci 12(2): 93-104.
  6. Boateng, K. A., Yang, X., Dong, F., Owen, H. A. and Makaroff, C. A.* (2008). SWI1 is required for meiotic chromosome remodeling events. Mol Plant 1(4): 620-633. (*Co-first author)
  7. Yang, X., Makaroff, C. A. and Ma, H. (2003). The Arabidopsis MALE MEIOCYTE DEATH1 gene encodes a PHD-finger protein that is required for male meiosis. Plant Cell 15(6): 1281-1295.
  8. Yang, X., Timofejeva, L., Ma, H. and Makaroff, C. A. (2006). The Arabidopsis SKP1 homolog ASK1 controls meiotic chromosome remodeling and release of chromatin from the nuclear membrane and nucleolus. J Cell Sci 119(Pt 18): 3754-3763.
  9. Yang, X., Ma, H. and Makaroff, C. A. (2004). Characterization of an unusual Ds transposable element in Arabidopsis thaliana: insertion of an abortive circular transposition intermediate. Plant Mol Biol 55(6): 905-917.
  10. Lam, W. S., Yang, X. and Makaroff, C. A. (2005). Characterization of Arabidopsis thaliana SMC1 and SMC3: evidence that AtSMC3 may function beyond chromosome cohesion. J Cell Sci 118(Pt 14): 3037-3048.
  11. Zhao, D., Yang, X., Quan, L., Timofejeva, L., Rigel, N. W., Ma, H. and Makaroff, C. A. (2006). ASK1, a SKP1 homolog, is required for nuclear reorganization, presynaptic homolog juxtaposition and the proper distribution of cohesin during meiosis in Arabidopsis. Plant Mol Biol 62(1-2): 99-110.
  12. Li, W., Yang, X., Lin, Z., Timofejeva, L., Xiao, R., Makaroff, C. A. and Ma, H. (2005). The AtRAD51C gene is required for normal meiotic chromosome synapsis and double-stranded break repair in Arabidopsis. Plant Physiol 138(2): 965-976.
  13. Li, W., Yang, X., Pua, E. and Goh, C. (1998). Isolation and characterization of cDNA clone encoding 1-aminocyclopropane-1-carboxylate oxidase from orchid (SW. Dendrobium crumenatum ). Plant Physiol. 119:805.
  14. Li, W., Yang, X., Pua, E.  and Goh, C. (1996). Isolation and characterization of cDNA clone encoding 1-aminocyclopropane-1-carboxylate synthase from orchid (SW. Dendrobium crumenatum ). Plant Physiol. 112:863.
  15. Li, W., Yang, X., Li, H. and Hu, D. (1993). Enhancing wheat protoplast isolation by negative pressure treatment. Wheat, Barley and Triticale Abstracts. International Maize and Wheat Improvement Center Vol. 10, No. 3: 249.
  16. Li, W., Yang, X. and Hu, D. (1991). Study on factors affecting pollen callus formation in barley. Acta Agriculturae Boreali-Sinica 6(1): 87-95.
  17. Li, W., Yang, X. and Hu, D. (1991). Enhancement of protoplast isolation efficiency by the treatment of negative pressure in wheat. Acta Agriculturae Boreali-Sinica 6(1): 57-61.
  18. Li, H., Li, W., Yang, X., Hu, D. and Liu, J. (1991). Protoplast culture and plantlet regeneration in winter wheat. Acta Botanica Sinica 33(9): 699-704.
1 Protocol published
Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Authors:  Pablo Bolaños-Villegas, Xiaohui Yang, Christopher A. Makaroff and Guang-Yuh Jauh, date: 04/20/2014, view: 6243, Q&A: 0
This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the ...