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Coauthors
Marisa Rosa University of California, Berkeley
2 protocols

Ortrun Mittelsten Scheid
  • Gregor Mendel Institute of Molecular Plant Biology, Austria
  • 2 Author merit
2 Protocols published
Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 4800, Q&A: 0
Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are ...
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 4117, Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a ...