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Coauthors
Deyin Guo College of Life Sciences, Wuhan University, China
1 protocol
Coreviewers
Andrea Puhar MIMS - Umea University
18 protocols

Chang Ho Lee Dankook University
4 protocols

HongLok Lung University of Hong Kong
56 protocols

Reviewer
Yu Chen
  • Professor, Wuhan University
Research focus
  • Molecular biology
  • virology; innate immunity
  • 1 Author merit
1 Protocol published
Biochemical Assays for MTase Activity
Authors:  Yu Chen and Deyin Guo, date: 01/20/2014, view: 3006, Q&A: 0
Methyltransferase (MTase) transfers a methyl group (-CH3) from the donor S-adenosyl-L-methionine (AdoMet or SAM) to biologically active molecules such as hormones, neurotransmitters, lipids, proteins and nucleic acids. The addition of a methyl group ...
4 Protocols reviewed
Ubiquitination Assay for Mammalian Cells
Authors:  Yang Peng, Edward Wang, Guang Peng and Shiaw-Yih Lin, date: 07/20/2016, view: 3556, Q&A: 0
Ubiquitin is an 8.5 kDa protein that can be activated and conjugated by ubiquitin-activating enzyme E1 and ubiquitin-conjugating enzyme E2, respectively. Ubiquitin E3 ligases then recognize protein substrates, and then transfer the ubiquitin from E2 ...
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Primer Extension Reactions for the PCR- based α- complementation Assay
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 2886, Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
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