Search
Coauthors
Deyin Guo College of Life Sciences, Wuhan University, China
1 protocol
Coreviewers
Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umea University, Sweden
13 protocols

Chang Ho Lee Molecular Biology Department, Dankook University, Republic of Korea
4 protocols

HongLok Lung Department of Clinical Oncology and Center for Cancer Research, The University of Hong Kong, Hong Kong SAR
56 protocols

Yu Chen
  • Professor, College of Life Sciences, Wuhan University, China
Research focus
  • Molecular biology
  • virology; innate immunity
Contributions
  • 1 Author merit
  • 4 Reviewer merit
1 Protocol published
Biochemical Assays for MTase Activity
Authors:  Yu Chen and Deyin Guo, date: 01/20/2014, view: 2769, Q&A: 0
Methyltransferase (MTase) transfers a methyl group (-CH3) from the donor S-adenosyl-L-methionine (AdoMet or SAM) to biologically active molecules such as hormones, neurotransmitters, lipids, proteins and nucleic acids. The addition of a methyl group ...
4 Protocols reviewed
Ubiquitination Assay for Mammalian Cells
Authors:  Yang Peng, Edward Wang, Guang Peng and Shiaw-Yih Lin, date: 07/20/2016, view: 2798, Q&A: 0
Ubiquitin is an 8.5 kDa protein that can be activated and conjugated by ubiquitin-activating enzyme E1 and ubiquitin-conjugating enzyme E2, respectively. Ubiquitin E3 ligases then recognize protein substrates, and then transfer the ubiquitin from E2 ...
More >
Primer Extension Reactions for the PCR- based α- complementation Assay
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 2507, Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
More >