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Alka Mehra Institute of Genomics and Integrative Biology
14 protocols

Cody Kime Center of Developmental Biology, RIKEN
1 protocol

Cristina Rohena University of California, San Francisco
1 protocol

Diana Búzová Global Change Research Institute CAS
1 protocol

Editor
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 4719, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
4 Protocols reviewed
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Authors:  Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 1698, Q&A: 0
Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...
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20 Protocols edited
Monitoring the Targeting of Cathepsin D to the Lysosome by Metabolic Labeling and Pulse-chase Analysis
Authors:  Lucas A. Tavares and Luis L. P. daSilva, date: 11/05/2017, view: 408, Q&A: 0
Mannose 6-phosphate receptors function can be studied in living cells by investigating alterations in processing and secretion of their ligand Cathepsin D. The assay described here is well established in the literature and comprises the metabolic ...
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In vitro NLK Kinase Assay
Authors:  Sungho Moon, Jiyoung Kim and Eek‐hoon Jho, date: 11/05/2017, view: 376, Q&A: 0
This protocol provides step by step instructions to perform an in vitro kinase assay for nemo-like kinase. In addition, this protocol also describes an efficient method using mild lysis buffer for expression and purification of Glutathione ...
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