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Alka Mehra Department of Allergy and Infectious Diseases, Institute of Genomics and Integrative Biology, Mall Road, Delhi, India
10 protocols

Cody Kime Lab of Retinal Regeneration, CDB/RIKEN
1 protocol

Cristina Rohena UCSD
1 protocol

Diana Búzová Global Change Research Institute CAS, Czech republic
1 protocol

Ralph Thomas Boettcher
  • Molecular Medicine Division, Max Planck Institute for Biochemistry, Germany
Research focus
  • Cell biology
Contributions
  • 1 Author merit
  • 4 Reviewer merit
  • 18 Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 4369, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
4 Protocols reviewed
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Authors:  Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 1310, Q&A: 0
Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...
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18 Protocols edited
Semi-quantitative Analysis of H4K20me1 Levels in Living Cells Using Mintbody
Authors:  Yuko Sato and Hiroshi Kimura, date: 05/20/2017, view: 965, Q&A: 0
Eukaryotic nuclear DNA wraps around histone proteins to form a nucleosome, a basic unit of chromatin. Posttranslational modification of histones play an important role in gene regulation and chromosome duplication. Some modifications are quite ...
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Relative Stiffness Measurements of Tumour Tissues by Shear Rheology
Authors:  Chris D. Madsen and Thomas R. Cox, date: 05/05/2017, view: 1130, Q&A: 0
The microenvironment of solid tumours is a critical contributor to the progression of tumours and offers a promising target for therapeutic intervention (Cox and Erler, 2011; Barker et al., 2012; Cox et al., 2016; Cox and Erler, ...
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