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Alizée Malnoë UC Berkeley, Dept. of Plant & Microbial Biology
2 protocols

Annis Richardson UC Berkeley
3 protocols

Arsalan Daudi Department of Plant Pathology, University of California, Davis, CA, USA
137 protocols

Baohua Li UC Davis
4 protocols

Renate Weizbauer
  • Post-Doc, Carnegie Insitution for Science, Stanford, CA
Research focus
  • Plant science
Contributions
  • 15 Reviewer merit
  • 24 Editor merit

Education

Ph.D., Purdue University

15 Protocols reviewed
Plant Tissue Trypan Blue Staining During Phytopathogen Infection
In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and ...
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A Ribosome Footprinting Protocol for Plants
Authors:  Catharina Merchante, Qiwen Hu, Steffen Heber, Jose Alonso and Anna N. Stepanova, date: 11/05/2016, view: 2161, Q&A: 0
Ribosome footprinting, or Ribo-seq, has revolutionized the studies of translation. It was originally developed for yeast and mammalian cells in culture (Ingolia et al., 2009). Herein, we describe a plant-optimized hands-on ribosome ...
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24 Protocols edited
CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae
Authors:  Yu Sasano and Satoshi Harashima, date: 05/20/2017, view: 1071, Q&A: 0
Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering ...
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Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi
Authors:  Noelia Lander, Miguel A. Chiurillo, Aníbal E. Vercesi and Roberto Docampo, date: 05/20/2017, view: 1577, Q&A: 0
To achieve the C-terminal tagging of endogenous proteins in T. cruzi we use the Cas9/pTREX-n vector (Lander et al., 2015) to insert a specific tag sequence (3xHA or 3xc-Myc) at the 3’ end of a specific gene of interest (GOI). ...
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