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William Plaxton Department of Biology, Queen’s University, Canada
3 protocols

Vicki Knowles
  • Department of Biology, Queen's University, Canada
  • 2 Author merit
2 Protocols published
Quantification of Total and Soluble Inorganic Phosphate
Authors:  Vicki Knowles and William Plaxton, date: 09/05/2013, view: 5692, Q&A: 0
A simple, rapid, and sensitive colorimetric microassay for inorganic phosphate (Pi) relies upon the absorption at 660 nm of a molybdenum blue complex that forms upon reduction of an ammonium molybdate-Pi complex in acid. The method for determination ...
Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration
Authors:  Vicki Knowles and William Plaxton, date: 09/05/2013, view: 8468, Q&A: 0
Acid phosphatases (APases) catalyze the hydrolysis of inorganic phosphate (Pi) from a broad range of Pi-monoesters with an acidic pH optimum. The liberated Pi is reassimilated into cellular metabolism via mitochondrial or chloroplastic ATP synthases ...