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Coauthors
Kouichi Funato Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
1 protocol

Kentaro Kajiwara Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan
1 protocol

Takefumi Karashima
  • Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
Contributions
  • 1 Author merit

Education

Master Degree, Graduate School of Biosphere Sciences, Hiroshima University

Current position

Master Student, Graduate School of Biosphere Sciences, Hiroshima University

Publications

  1. Kajiwara, K., Muneoka, T., Watanabe, Y., Karashima, T., Kitagaki, H. and Funato, K. (2012). Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast. Mol Microbiol 86(5): 1246-1261.
1 Protocol published
Metabolic Labeling of Yeast Sphingolipids with Radioactive D-erythro-[4,5-3H]dihydrosphingosine
Authors:  Takefumi Karashima, Kentaro Kajiwara and Kouichi Funato, date: 08/20/2013, view: 4262, Q&A: 0
Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of Saccharomyces cerevisiae with a radioactive ceramide precursor, ...