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Coauthors
Felipe Cruz-García Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Florencia García-Campusano Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Lilia Bernal Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Edgar Nájera
  • Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
Contributions
  • 1 Author merit
1 Protocol published
Reverse Zymmogram Analysis for the Detection of Protease Inhibitor Activity
Authors:  Lilia Bernal, Florencia García-Campusano, Edgar Nájera and Felipe Cruz-García, date: 08/20/2013, view: 4947, Q&A: 0
This protocol describes a gel-based procedure to detect protease inhibitor activity. In this method gelatin is used as a substrate for proteolysis and is copolymerized within the polyacrylamide matrix. Protein extracts are fractioned by SDS-PAGE and ...