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Coauthors
Sébastien Gélis-Jeanvoine Micalis INRA, Domaine de Vilvert, France
1 protocol

Tanneke den Blaauwen Bacterial Cell Biology Department, University of Amsterdam, The Netherlands
1 protocol

Mirjam Aarsman (Retired) Bacterial Cell Biology Department, University of Amsterdam, The Netherlands
1 protocol

Nienke Buddelmeijer
  • Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall Unit, INSERM Group Avenir, France
  • 2 Author merit
2 Protocols published
Substituted Cysteine Accessibility Method for Topology and Activity Studies of Membrane Enzymes Forming Thioester Acyl Intermediates in Bacteria
Authors:  Sébastien Gélis-Jeanvoine and Nienke Buddelmeijer, date: 11/05/2015, view: 2445, Q&A: 0
The topology of membrane proteins and enzymes can be determined using various methods including reporter protein fusions and accessibility of cysteine residues to alkylating agents. Here we describe a variation of the substituted cysteine ...
Immunolabeling of Proteins in situ in Escherichia coli K12 Strains
Authors:  Nienke Buddelmeijer, Mirjam Aarsman and Tanneke den Blaauwen, date: 08/05/2013, view: 4225, Q&A: 0
This protocol was developed to label proteins in bacterial cells with antibodies conjugated to a fluorophore for fluorescence microscopy imaging. The procedure is optimized to minimize morphological changes and also to minimize the amount of ...