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Coauthors
Luis Enjuanes Molecular and Cell Biology, CNB-CSIC, Spain
1 protocol

Pedro Mateos-Gomez Molecular and Cell Biology, CNB-CSIC, Spain
1 protocol

Lucia Morales Molecular and Cell Biology, CNB-CSIC, Spain
1 protocol

Isabel Sola
  • Molecular and Cell Biology, CNB-CSIC, Spain
  • 1 Author merit

Education

Ph.D in Science, Department of Molecular Biology, University Autonoma of Madrid, Spain, 1997

Current position

Scientist of the Spanish Research Council (CSIC) at the National Centre of Biotechnology (CNB-CSIC), Madrid, Spain

Publications (since 2008)

  1. Cruz, J.L., Becares, M., Sola, I., Oliveros, J.C., Enjuanes, L., and Zuniga, S. (2013). Alphacoronavirus protein 7 modulates host innate immune response. J Virol. In press
  2. Mateos-Gomez, P. A., Morales, L., Zuniga, S., Enjuanes, L. and Sola, I. (2013). Long-distance RNA-RNA interactions in the coronavirus genome form high-order structures promoting discontinuous RNA synthesis during transcription. J Virol 87(1): 177-186.
  3. Mateos-Gomez, P. A., Zuniga, S., Palacio, L., Enjuanes, L. and Sola, I. (2011). Gene N proximal and distal RNA motifs regulate coronavirus nucleocapsid mRNA transcription. J Virol 85(17): 8968-8980.
  4. Cruz, J. L., Sola, I., Becares, M., Alberca, B., Plana, J., Enjuanes, L. and Zuniga, S. (2011). Coronavirus gene 7 counteracts host defenses and modulates virus virulence. PLoS Pathog 7(6): e1002090.
  5. Sola, I., Galan, C., Mateos-Gomez, P. A., Palacio, L., Zuniga, S., Cruz, J. L., Almazan, F. and Enjuanes, L. (2011). The polypyrimidine tract-binding protein affects coronavirus RNA accumulation levels and relocalizes viral RNAs to novel cytoplasmic domains different from replication-transcription sites. J Virol 85(10): 5136-5149.
  6. Dufour, D., Mateos-Gomez, P. A., Enjuanes, L., Gallego, J. and Sola, I. (2011). Structure and functional relevance of a transcription-regulating sequence involved in coronavirus discontinuous RNA synthesis. J Virol 85(10): 4963-4973.
  7. Sola, I., Mateos-Gomez, P. A., Almazan, F., Zuniga, S. and Enjuanes, L. (2011). RNA-RNA and RNA-protein interactions in coronavirus replication and transcription. RNA Biol 8(2): 237-248.
  8. Cruz, J. L., Zuniga, S., Becares, M., Sola, I., Ceriani, J. E., Juanola, S., Plana, J. and Enjuanes, L. (2010). Vectored vaccines to protect against PRRSV. Virus Res 154(1-2): 150-160.
  9. Zuniga, S., Cruz, J. L., Sola, I., Mateos-Gomez, P. A., Palacio, L. and Enjuanes, L. (2010). Coronavirus nucleocapsid protein facilitates template switching and is required for efficient transcription. J Virol 84(4): 2169-2175.
  10. Galan, C., Sola, I., Nogales, A., Thomas, B., Akoulitchev, A., Enjuanes, L. and Almazan, F. (2009). Host cell proteins interacting with the 3' end of TGEV coronavirus genome influence virus replication. Virology 391(2): 304-314.
  11. Zuniga, S., Sola, I., Cruz, J. L. and Enjuanes, L. (2009). Role of RNA chaperones in virus replication. Virus Res 139(2): 253-266.
  12. Moreno, J. L., Zuniga, S., Enjuanes, L. and Sola, I. (2008). Identification of a coronavirus transcription enhancer. J Virol 82(8): 3882-3893.
1 Protocol published
RNA-Affinity Chromatography
Authors:  Lucia Morales , Pedro A. Mateos-Gomez, Luis Enjuanes and Isabel Sola, date: 07/05/2013, view: 4660, Q&A: 0
RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins ...