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Coauthors
Mário Ramirez Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Portugal
1 protocol

José Melo-Cristino Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Portugal
1 protocol

Reviewer
Maria João Frias
  • Universidade de Lisboa
Research focus
  • Microbiology
Contributions
  • 1 Author merit

Education

Ph.D. in Health Sciences and Technology, specialty of Microbiology, Faculty of Medicine, University of Lisbon, Portugal, 2011

Current position

Postdoctoral Researcher, Institute of Molecular Medicine (IMM), Faculty of Medicine, University of Lisbon, Portugal

Teaching assistant 30% in Microbiology (BSc in Health Sciences), Institute of Microbiology, Faculty of Medicine, University of Lisbon, Portugal

Publications

  1. Frias, M. J., Melo-Cristino, J. and Ramirez, M. (2013). Export of the pneumococcal phage SV1 lysin requires choline-containing teichoic acids and is holin-independent. Mol Microbiol 87(2): 430-445.
  2. Carrolo, M.*, Frias, M. J.*, Pinto, F. R., Melo-Cristino, J. and Ramirez, M. (2010). Prophage spontaneous activation promotes DNA release enhancing biofilm formation in Streptococcus pneumoniae. PLoS One 5(12): e15678.
    *These authors contributed equally to this work.
  3. Frias, M. J., Melo-Cristino, J. and Ramirez, M. (2009). The autolysin LytA contributes to efficient bacteriophage progeny release in Streptococcus pneumoniae. J Bacteriol 191(17): 5428-5440.
  4. Aguiar, S. I., Frias, M. J., Santos, L., Melo-Cristino, J., Ramirez, M. and Portuguese Surveillance Group For Study Of Respiratory, P. (2006). Emergence of optochin resistance among Streptococcus pneumoniae in Portugal. Microb Drug Resist 12(4): 239-245.
  5. Marques, A. R., Espadinha, C., Frias, M. J., Roque, L., Catarino, A. L., Sobrinho, L. G. and Leite, V. (2004). Underexpression of peroxisome proliferator-activated receptor (PPAR)gamma in PAX8/PPARgamma-negative thyroid tumours. Br J Cancer 91(4): 732-738.
1 Protocol published
Preparation of Pneumococcal Proteins for Western Blot Analysis
Authors:  Maria João Frias, José Melo-Cristino and Mário Ramirez, date: 07/05/2013, view: 3783, Q&A: 0
This protocol was developed in a study aimed to determine the cellular localization of the lysin of pneumococcal phage SV1 (Frias et al., 2013). We obtained proteins from the surface of Streptococcus pneumoniae by elution with ...