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Coauthors
Patrick Sexton Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
2 protocols

Cassandra Koole Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
2 protocols

Denise Wootten
  • Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
Contributions
  • 2 Author merit
2 Protocols published
pERK Detection Assays Using the Surefire AlphaScreen® Kit (TGR Biosciences and PerkinElmer)
Authors:  Cassandra Koole, Denise Wootten and Patrick Sexton, date: 07/05/2013, view: 4247, Q&A: 0
Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are serine/threonine protein kinases that are phosphorylated on Thr202/Tyr204 (ERK1) and Thr185/Tyr187 (ERK2). Phosphorylation of ERK1/2 (pERK1/2) arises from multiple stimuli, resulting in ...
cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
Authors:  Cassandra Koole, Denise Wootten and Patrick Sexton, date: 07/05/2013, view: 5618, Q&A: 0
Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi ...