Maureen Wirschell Department of Biochemistry, University of Mississippi Medical Center, USA
1 protocol

Mary E. Porter
  • Department of Genetics, Cell Biology, and Development, University of Minnesota, USA
  • 1 Author merit


Ph.D. in Biology, University of Pennsylvania, Philadelphia, PA, 1982

Current position

Professor, Dept of Genetics, Cell Biology & Development, UMN, Minneapolis, MN


  1. Wirschell, M., Olbrich, H., Werner, C., Tritschler, D., Bower, R., Sale, W. S., Loges, N. T., Pennekamp, P., Lindberg, S., Stenram, U., Carlen, B., Horak, E., Kohler, G., Nurnberg, P., Nurnberg, G., Porter, M. E. and Omran, H. (2013). The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans. Nat Genet 45(3): 262-268.
  2. Bower, R., Tritschler, D., Vanderwaal, K., Perrone, C. A., Mueller, J., Fox, L., Sale, W. S. and Porter, M. E. (2013). The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24(8): 1134-1152.
  3. O'Toole, E. T., Giddings, T. H., Jr., Porter, M. E. and Ostrowski, L. E. (2012). Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure. Cytoskeleton (Hoboken) 69(8): 577-590.
  4. Heuser, T., Barber, C. F., Lin, J., Krell, J., Rebesco, M., Porter, M. E. and Nicastro, D. (2012). Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein. Proc Natl Acad Sci U S A 109(30): E2067-2076.
  5. Toba, S., Fox, L. A., Sakakibara, H., Porter, M. E., Oiwa, K. and Sale, W. S. (2011). Distinct roles of 1alpha and 1beta heavy chains of the inner arm dynein I1 of Chlamydomonas flagella. Mol Biol Cell 22(3): 342-353.
  6. VanderWaal, K. E., Yamamoto, R., Wakabayashi, K., Fox, L., Kamiya, R., Dutcher, S. K., Bayly, P. V., Sale, W. S. and Porter, M. E. (2011). bop5 Mutations reveal new roles for the IC138 phosphoprotein in the regulation of flagellar motility and asymmetric waveforms. Mol Biol Cell 22(16): 2862-2874.
  7. Lin, J., Tritschler, D., Song, K., Barber, C. F., Cobb, J. S., Porter, M. E. and Nicastro, D. (2011). Building blocks of the nexin-dynein regulatory complex in Chlamydomonas flagella. J Biol Chem 286(33): 29175-29191.
  8. Nicastro, D., Fu, X., Heuser, T., Tso, A., Porter, M. E. and Linck, R. W. (2011). Cryo-electron tomography reveals conserved features of doublet microtubules in flagella. Proc Natl Acad Sci U S A 108(42): E845-853.
  9. Hom, E. F., Witman, G. B., Harris, E. H., Dutcher, S. K., Kamiya, R., Mitchell, D. R., Pazour, G. J., Porter, M. E., Sale, W. S., Wirschell, M., Yagi, T. and King, S. M. (2011). A unified taxonomy for ciliary dyneins. Cytoskeleton (Hoboken) 68(10): 555-565.
  10. Wirschell, M., Yang, C., Yang, P., Fox, L., Yanagisawa, H. A., Kamiya, R., Witman, G. B., Porter, M. E. and Sale, W. S. (2009). IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding. Mol Biol Cell 20(13): 3044-3054.
  11. Bower, R., VanderWaal, K., O'Toole, E., Fox, L., Perrone, C., Mueller, J., Wirschell, M., Kamiya, R., Sale, W. S. and Porter, M. E. (2009). IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20(13): 3055-3063.
  12. Heuser, T., Raytchev, M., Krell, J., Porter, M. E. and Nicastro, D. (2009). The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella. J Cell Biol 187(6): 921-933.
  13. Nicastro, D., Schwartz, C., Pierson, J., Gaudette, R., Porter, M. E. and McIntosh, J. R. (2006). The molecular architecture of axonemes revealed by cryoelectron tomography. Science 313(5789): 944-948.
  14. Mueller, J., Perrone, C. A., Bower, R., Cole, D. G. and Porter, M. E. (2005). The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16(3): 1341-1354.
  15. Hendrickson, T. W., Perrone, C. A., Griffin, P., Wuichet, K., Mueller, J., Yang, P., Porter, M. E. and Sale, W. S. (2004). IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending. Mol Biol Cell 15(12): 5431-5442.
  16. Perrone, C. A., Tritschler, D., Taulman, P., Bower, R., Yoder, B. K. and Porter, M. E. (2003). A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and Mammalian cells. Mol Biol Cell 14(5): 2041-2056.
  17. Rupp, G. and Porter, M. E. (2003). A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162(1): 47-57.
  18. Rupp, G., O'Toole, E. and Porter, M. E. (2001). The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility. Mol Biol Cell 12(3): 739-751.
  19. Perrone, C. A., Myster, S. H., Bower, R., O'Toole, E. T. and Porter, M. E. (2000). Insights into the structural organization of the I1 inner arm dynein from a domain analysis of the 1beta dynein heavy chain. Mol Biol Cell 11(7): 2297-2313.
  20. Myster, S. H., Knott, J. A., Wysocki, K. M., O'Toole, E. and Porter, M. E. (1999). Domains in the 1alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas. J Cell Biol 146(4): 801-818.
  21. Porter, M. E., Bower, R., Knott, J. A., Byrd, P. and Dentler, W. (1999). Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas. Mol Biol Cell 10(3): 693-712.
  22. Perrone, C. A., Yang, P., O'Toole, E., Sale, W. S. and Porter, M. E. (1998). The Chlamydomonas IDA7 locus encodes a 140-kDa dynein intermediate chain required to assemble the I1 inner arm complex. Mol Biol Cell 9(12): 3351-3365.
  23. Myster, S. H., Knott, J. A., O'Toole, E. and Porter, M. E. (1997). The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex. Mol Biol Cell 8(4): 607-620.
1 Protocol published
Generation of Polyclonal Specific Antibodies
Authors:  Maureen Wirschell and Mary E. Porter, date: 06/05/2013, view: 4195, Q&A: 0
Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The ...