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Coauthors
Joerg Nickelsen Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
2 protocols

Alexandra Bohne Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
1 protocol

Christian Schwarz
  • Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
Contributions
  • 2 Author merit

Education

Ph.D. in Plant Molecular Biology, Ludwig-Maximilians-University Munich

Current position

Postdoctoral researcher in Molecular Plant Science at the Ludwig-Maximilians-University, Munich

Publications (since 2005)

  1. Bohne, A.-V., Schwarz, C., Schottkowski, M., Lidschreiber, M., Piotrowski, M., Zerges, W. and Nickelsen, J. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLoS biology 11(2): e1001482.
  2. Schwarz, C., Bohne, A. V., Wang, F., Cejudo, F. J. and Nickelsen, J. (2012). An intermolecular disulfide‐based light switch for chloroplast psbD gene expression in Chlamydomonas reinhardtii. The Plant Journal 72(3): 378-389.
  3. Schwarz, C. and Nickelsen, J. (2010). Enrichment of native, high molecular weight ribonucleoprotein complexes from chloroplasts by consecutive gel filtration steps. Analysis of factors involved in plastid gene expression in the unicellular green alga Chlamydomonas reinhardtii 89: 160.    
  4. Dorn, K. V., Willmund, F., Schwarz, C., Henselmann, C., Pohl, T., HeB, B., Veyel, D., Usadel, B., Friedrich, T. and Nickelsen, J. (2010). Chloroplast DnaJ-like proteins 3 and 4 (CDJ3/4) from Chlamydomonas reinhardtii contain redox-active Fe-S clusters and interact with stromal HSP70B. Biochemical Journal 427(2): 205-215.    
  5. Johnson, X., Wostrikoff, K., Finazzi, G., Kuras, R., Schwarz, C., Bujaldon, S., Nickelsen, J., Stern, D. B., Wollman, F.-A. and Vallon, O. (2010). MRL1, a conserved pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis. The Plant Cell Online 22(1): 234-248.
  6. Wobbe, L., Blifernez, O., Schwarz, C., Mussgnug, J. H., Nickelsen, J. and Kruse, O. (2009). Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proceedings of the National Academy of Sciences 106(32): 13290-13295.
  7. Bohne, A.-V., Schwarz, C., Jalal, A., Ossenbühl, F. and Nickelsen, J. (2009) Control of organellar gene expression in Chlamydomonas – future perspectives -. Endocyt. Cell Res. 19, 70-80.
  8. Wobbe, L., Schwarz, C., Nickelsen, J. and Kruse, O. (2008). Translational control of photosynthetic gene expression in phototrophic eukaryotes. Physiol Plant 133(3): 507-515.
  9. Schwarz, C., Elles, I., Kortmann, J., Piotrowski, M. and Nickelsen, J. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell 19(11): 3627-3639.
  10. Balczun, C., Bunse, A., Schwarz, C., Piotrowski, M. and Kück, U. (2006). Chloroplast heat shock protein Cpn60 from Chlamydomonas reinhardtii exhibits a novel function as a group II intron-specific RNA-binding protein. FEBS Lett 580(18): 4527-4532.    
  11. Klinkert, B., Schwarz, C., Pohlmann, S., Pierre, Y., Girard-Bascou, J. and Nickelsen, J. (2005). Relationship between mRNA levels and protein accumulation in a chloroplast promoter-mutant of Chlamydomonas reinhardtii. Molecular Genetics and Genomics 274(6): 637-643.
2 Protocols published
35S pulse Labelling of Chlamydomonas Chloroplast Proteins
Authors:  Alexandra-Viola Bohne, Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 4391, Q&A: 0
35S pulse labelling of proteins is used to attach a radioactive label to newly synthesized proteins, as sulfur is an element that is mainly present in proteins (Fleischmann and Rochaix 1999). Depending on your organism’s uptake mechanisms ...
2D Diagonal Redox SDS-PAGE of Proteins
Authors:  Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 6160, Q&A: 0
2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a ...